RESUMO
PURPOSE: The purpose of this investigation was to determine the expression and significance of tumor associated macrophages (TAMs) and CXCR4 in non-small cell lung cancer (NSCLC). METHODS: Immunohistochemical staining was used to analyze the expression of CD68 (TAM surface marker) and CXCR4 in 68 cases of NSCLC and 17 cases of normal lung tissue. RESULTS AND CONCLUSION: The positive rate of CD68 was 66.2% (45/68) and CXCR4 was 61.8% (42/68) in the lung cancer tissues, while the rates in normal tissues were statistically significantly lower at 27.3% (3/17) and 11.8% (2/17), respectively. The tissue expressions of CD68 and CXCR4 in NSCLC tissues were significantly correlated with a higher TNM staging and lymph node metastasis (p<0.05); and the expression of CD68 was positively correlated with the expression of CXCR4 (r=0.461, p=0.000). The TAMs infiltration and the expression of CXCR4 were closely correlated with the cellular carcinogenesis and development of NSCLC and their combined detection could provide a potential target for clinical diagnosis and adjuvant therapy for patient with NSCLC.
Assuntos
Antígenos CD/genética , Antígenos de Diferenciação Mielomonocítica/genética , Carcinoma Pulmonar de Células não Pequenas/genética , Macrófagos/metabolismo , Receptores CXCR4/genética , Idoso , Biomarcadores Tumorais/genética , Carcinoma Pulmonar de Células não Pequenas/patologia , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Estimativa de Kaplan-Meier , Metástase Linfática , Macrófagos/patologia , Masculino , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Prognóstico , Transdução de SinaisRESUMO
According to the results of analyzing the microwave-induced thermoacoustic signals, the amplitude and frequency were estimated and the designing parameters of preprocessor were decided. Based on the parameters, the signals preprocessor was designed with the band pass frequency ranging from 50 KHz to 3MHz, the gain ranging from 55 dB to 105 dB and the output noise being 1.32 V when the input was zero and the gain was 105 dB. The de-noising method of thermoacoustic signals was also discussed. The signals can be picked up by the preprocessor combined with the digital multipoint average method. The amplitude of signals is only 5 microV or even less. The results indicated that the preprocessor can meet the needs of thermoacoustic signals acquisition in bandwidth, gain and noise control.
Assuntos
Acústica , Algoritmos , Fenômenos Biofísicos , Desenho Assistido por Computador , Desenho de Equipamento , Micro-Ondas , Termografia , Métodos , Tomografia , MétodosRESUMO
In order to improve the noise ratio and stability of the amplified small-signal in microwave-induced thermoacoustic tomography,an amplifying circuit with wide band and high voltage gain is designed in this paper.The results show that the voltage gain is up to 80dB and the bandwidth is about 7MHz.
