RESUMO
The COVID-19 pandemic has accounted for more than five million infections and hundreds of thousand deaths worldwide in the past six months. The patients demonstrate a great diversity in clinical and laboratory manifestations and disease severity. Nonetheless, little is known about the host genetic contribution to the observed inter-individual phenotypic variability. Here, we report the first host genetic study in China by deeply sequencing and analyzing 332 COVID-19 patients categorized by varying levels of severity from the Shenzhen Third Peoples Hospital. Upon a total of 22.2 million genetic variants, we conducted both single-variant and gene-based association tests among five severity groups including asymptomatic, mild, moderate, severe and critical ill patients after the correction of potential confounding factors. The most significant gene locus associated with severity is located in TMEM189-UBE2V1 involved in the IL-1 signaling pathway. The p.Val197Met missense variant that affects the stability of the TMPRSS2 protein displays a decreasing allele frequency among the severe patients compared to the mild and the general population. We also identified that the HLA-A*11:01, B*51:01 and C*14:02 alleles significantly predispose the worst outcome of the patients. This initial study of Chinese patients provides a comprehensive view of the genetic difference among the COVID-19 patient groups and highlighted genes and variants that may help guide targeted efforts in containing the outbreak. Limitations and advantages of the study were also reviewed to guide future international efforts on elucidating the genetic architecture of host-pathogen interaction for COVID-19 and other infectious and complex diseases.
RESUMO
To study the effect of physical fitness exercise prescription on the cardiac function of collegians, three different exercise prescriptions on the collegians extra-curriculum exercises to develop their strength, cardiac function and overall physical fitness separately and one control were applied. The results show that after 8 weeks training, the rest HR of all exercise groups decreased while SV(stroke volumn), SI (stroke index), CO (cardiac output)and CI (cardiac index) increased significantly. Furthermore, the results also show that VPE (ventricular pumping effectiveness) and VPEI (ventricular pumping effectiveness index) of exercise groups increased while MSP(mean systolic pressure), MDP(mean diastolic pressure) and MAP (mean arterial pressure) decreased at variant degree. From the study it is not difficult to see that doing exercise prescription can develop the collegians' cardiac-function significantly. Comparing these three exercise prescriptions show that doing endurance exercise prescription can get the best effect on developing the cardiac-function, but doing the comprehensive-developing exercise prescription can get more comprehensive effect on cardiac and vascular function. Another result from comparing the control group shows that the females are easier to get the physiological function descent than the males if they do not do exercises actively. In conclusion, it is worth to extend physical fitness exercise prescription in collegians' extra-curriculum exercise because of the simple condition, scientific method and significant effect.