RESUMO
Stress-induced premature senescence (SIPS) model is in vitro model of cellular aging. In this study, apoptosis was evaluated in SIPS model and in replicative senescent fibroblasts. We also compared the activity of senescence-associated beta-galactosidase (SA-beta gal) as a biomarker of cellular aging. Our results suggested that SIPS model and senescent fibroblasts might share similar mechanism of aging and apoptosis pathway.
Assuntos
Envelhecimento/fisiologia , Apoptose/efeitos dos fármacos , Senescência Celular/efeitos dos fármacos , Fibroblastos/efeitos dos fármacos , Fenômenos Fisiológicos da Pele/efeitos dos fármacos , Pele/efeitos dos fármacos , beta-Galactosidase/biossíntese , Envelhecimento/efeitos dos fármacos , Anexinas , Biomarcadores , Circuncisão Masculina , Prepúcio do Pênis/fisiologia , Humanos , Peróxido de Hidrogênio , Técnicas In Vitro , Masculino , Modelos Teóricos , beta-Galactosidase/efeitos dos fármacosRESUMO
Validation of housekeeping gene is important for accurate quantitation of RNA in real time RT-PCR technique. The purpose of this study was to determine the validity of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a housekeeping gene for quantitative real time RT-PCR assessment in human skin fibroblast senescent model. The cells were divided into different treatment groups; young (passage 4), senescent (passage 30), treatment with H2O2 and treatment with A-tocotrienol prior to H2O2 treatment. Our results showed that the expression level of GAPDH was constant with different treatment groups. Therefore, we concluded that GAPDH was suitable to be used as housekeeping gene in human skin fibroblast senescent model.
