Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 8 de 8
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Cesk Epidemiol Mikrobiol Imunol ; 39(6): 327-34, 1990 Nov.
Artigo em Tcheco | MEDLINE | ID: mdl-2150622

RESUMO

Between reactions assessing one class of immunoglobulins and reactions detecting all serum immunoglobulins comprehensively such as CFT, there is, as might be expected, a very poor quantitative correlation and thus in individual sera the result of one reaction cannot be reliably added to the result of another (CFT:ELISA/IgG). Even the correlation between reactions focused on the same Ig class (ELISA/IgG: IFT/IgG) is no better. Among reactions assessing specific IgM we consider ELISA/IgM better than IFT/IgM because there is not the risk of false negativity caused by concurrence of IgG. A combination of ELISA/IgG and ELISA/IgM gives good results as a statistical group: the distribution of results revealed agglomerations of sera corresponding to the assumed age of the infection derived from the generally accepted pattern of antibody formation. The applicability of the combination of these two reactions alone for evaluation of individual sera is a promising procedure but awaits further confirmation. Long-term investigations revealed within two years after infection a marked decline of CFT antibodies in the majority of cases but it was not sufficiently clear in ELISA/IgG. Despite the technical advantages of ELISA reactions, elimination af the CFT reaction is not foreseen in the near future. As the minimal combination of methods which provides adequate information we may consider at the present time CFT for assessment of total antibodies and ELISA/IgM for more marked differentiation of the acute stage. Evaluation of the lowest CFR titres considered hitherto as "practically negative" must be obviously revised in subjects with immunosuppression and organ donors for transplantation.(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Anticorpos Antiprotozoários/análise , Toxoplasma/imunologia , Animais , Testes de Fixação de Complemento , Ensaio de Imunoadsorção Enzimática , Humanos , Imunoglobulina A/análise , Imunoglobulina M/análise , Toxoplasmose/diagnóstico , Toxoplasmose/imunologia
2.
Vet Med Nauki ; 14(4): 31-9, 1977.
Artigo em Búlgaro | MEDLINE | ID: mdl-412294

RESUMO

A total of 698 blood serum samples from sheep in various regions of the People's Republic of Bulgaria were examined throught the indirect hemaglutination micromethod. Two-hundred and seventeen proved positive for toxoplasmosis. Those of the samples that were taken from ewes that had miscarried and reacted positively were considerably more--43.3 per cent--than those that were taken from ewes with normal lambing and reacted positively--21 percent. Besides, toxoplasmosis was shown to be more widely spread among sheep in the mountainous regions (40.3 per cent) than in sheep from the plain regions (18.3 per cen). Four cystogenic strains of Toxoplasma were isolated from fetuses of nine ewes that had miscarried. Out of 493 sheep investigated in Czechoslovakia 33.3 per cent were positive by the indirect hemagglutination reaction, 29.7 per cent were positive by the indirect fluorescence reaction (RIF), and 11.6 per cent were positive by thr microprecipitation agar gel reaction (MPA). States is the epizootiologic and economic importance of the problem.


Assuntos
Doenças dos Ovinos/epidemiologia , Toxoplasmose Animal/epidemiologia , Aborto Animal/etiologia , Aborto Animal/microbiologia , Animais , Bulgária , Bovinos , Tchecoslováquia , Feminino , Testes de Hemaglutinação , Imunodifusão , Masculino , Gravidez , Complicações Infecciosas na Gravidez/veterinária , Ovinos , Doenças dos Ovinos/diagnóstico , Toxoplasma/isolamento & purificação , Toxoplasmose Animal/complicações , Toxoplasmose Animal/congênito , Toxoplasmose Animal/diagnóstico
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...