Bisphenols-A Threat to the Natural Environment.

Negative public sentiment built up around bisphenol A (BPA) follows growing awareness of the frequency of this chemical compound in the environment. The increase in air, water, and soil contamination by BPA has also generated the need to replace it with less toxic analogs, such as Bisphenol F (BPF) and Bisphenol S (BPS). However, due to the structural similarity of BPF and BPS to BPA, questions arise about the safety of their usage. The toxicity of BPA, BPF, and BPS towards humans and animals has been fairly well understood. The biodegradability potential of microorganisms towards each of these bisphenols is also widely recognized. However, the scale of their inhibitory pressure on soil microbiomes and soil enzyme activity has not been estimated. These parameters are extremely important in determining soil health, which in turn also influences plant growth and development. Therefore, in this manuscript, knowledge has been expanded and systematized regarding the differences in toxicity between BPA and its two analogs. In the context of the synthetic characterization of the effects of bisphenol permeation into the environment, the toxic impact of BPA, BPF, and BPS on the microbiological and biochemical parameters of soils was traced. The response of cultivated plants to their influence was also analyzed.

The influence of combined treatment of municipal wastewater and landfill leachate on the spread of antibiotic resistance in the environment - A preliminary case study.

Environmentally-friendly management of landfill leachate (LL) poses a challenge, and LL is usually co-treated with municipal wastewater in wastewater treatment plants (WWTPs). The extent to which the co-treatment of LL and municipal wastewater influences the spread of antibiotic resistance (AR) in the environment has not been examined to date. Two WWTPs with similar wastewater composition and technology were studied. Landfill leachate was co-treated with wastewater in one of the studied WWTPs. Landfill leachate, untreated and treated wastewater from both WWTPs, and river water sampled upstream and downstream from the wastewater discharge point were analyzed. Physicochemical parameters, microbial diversity, and antibiotic resistance genes (ARGs) abundance were investigated to determine the impact of LL co-treatment on chemical and microbiological contamination in the environment. Landfill leachate increased pollutant concentrations in untreated wastewater and river water. Cotreatment of LL and wastewater could affect the abundance and diversity of microbial communities and the interactions between microbial species. Co-treatment also decreased the stability of microbial co-occurrence networks in the examined samples. The mexF gene was identified as a potential marker of environmental pollution with LL. This is the first study to explore the impact of LL on the occurrence of AR determinants in wastewater and rivers receiving effluents.

Microbial Diversity and Enzyme Activity as Indicators of Permethrin-Exposed Soil Health.

Owing to their wide range of applications in the control of ticks and insects in horticulture, forestry, agriculture and food production, pyrethroids pose a significant threat to the environment, including a risk to human health. Hence, it is extremely important to gain a sound understanding of the response of plants and changes in the soil microbiome induced by permethrin. The purpose of this study has been to show the diversity of microorganisms, activity of soil enzymes and growth of Zea mays following the application of permethrin. This article presents the results of the identification of microorganisms with the NGS sequencing method, and of isolated colonies of microorganisms on selective microbiological substrates. Furthermore, the activity of several soil enzymes, such as dehydrogenases (Deh), urease (Ure), catalase (Cat), acid phosphatase (Pac), alkaline phosphatase (Pal), ß-glucosidase (Glu) and arylsulfatase (Aryl), as well as the growth of Zea mays and its greenness indicators (SPAD), after 60 days of growth following the application of permethrin, were presented. The research results indicate that permethrin does not have a negative effect on the growth of plants. The metagenomic studies showed that the application of permethrin increases the abundance of Proteobacteria, but decreases the counts of Actinobacteria and Ascomycota. The application of permethrin raised to the highest degree the abundance of bacteria of the genera Cellulomonas, Kaistobacter, Pseudomonas, Rhodanobacter and fungi of the genera Penicillium, Humicola, Iodophanus, Meyerozyma. It has been determined that permethrin stimulates the multiplication of organotrophic bacteria and actinomycetes, decreases the counts of fungi and depresses the activity of all soil enzymes in unseeded soil. Zea mays is able to mitigate the effect of permethrin and can therefore be used as an effective phytoremediation plant.

Assessment of biodegradability of cellulose and poly(butylene succinate)-based bioplastics under mesophilic and thermophilic anaerobic digestion with a view towards biorecycling.

Despite the increasing interest in bioplastics, there are still contradictory results on their actual biodegradability, which cause difficulties in choosing and developing appropriate sustainable treatment methods. Two biofoils (based on poly(butylene succinate) (PBS37) and cellulose (Cel37)) were anaerobically degraded during 100-day mesophilic (37 °C) and thermophilic (55 °C) tests (PBS55, Cel55). To overcome low degradation rates in mesophilic conditions, alkaline pre-treatment was also used (Pre-PBS37, Pre-Cel37). For comprehensive understanding of biodegradability, not only methane production (MP), but also the structure (topography, microscopic analysis), tensile properties, and FTIR spectra of the materials undergoing anaerobic degradation (AD) analysed. PBS37 and Pre-PBS37 were visible in 100-day degradation, and the cumulative MP reached 25.5 and 29.3 L/kg VS, respectively (4.3-4.9% of theoretical MP (TMP)). The biofoils started to show damage, losing their mechanical properties over 35 days. In contrast, PBS55 was visible for 14 days (cracks and fissures appeared), cumulative MP was 180.2 L/kg VS (30.2% of the TMP). Pieces of Cel were visible only during 2 days of degradation, and the MP was 311.4-315.0 L/kg VS (77.3-78.2% of the TMP) at 37 °C and 319.5 L/kg VS (79.3% of the TMP) at 55 °C. The FTIR spectra of Cel and PBS did not show shifts and formation of peaks. These findings showed differences in terms of the actual biodegradability of the bioplastics and provided a deeper understanding of their behaviour in AD, thus indicating limitations of AD as the final treatment of some materials, and also may support the establishment of guidelines for bioplastic management.

The Usability of Sorbents in Restoring Enzymatic Activity in Soils Polluted with Petroleum-Derived Products.

Due to their ability to adsorb or absorb chemical pollutants, including organic compounds, sorbents are increasingly used in the reclamation of soils subjected to their pressure, which results from their high potential in eliminating xenobiotics. The precise optimization of the reclamation process is required, focused primarily on restoring the condition of the soil. This research are essential for seeking materials sufficiently potent to accelerate the remediation process and for expanding knowledge related to biochemical transformations that lead to the neutralization of these pollutants. The goal of this study was to determine and compare the sensitivity of soil enzymes to petroleum-derived products in soil sown with Zea mays, remediated using four sorbents. The study was conducted in a pot experiment, with loamy sand (LS) and sandy loam (SL) polluted with VERVA diesel oil (DO) and VERVA 98 petrol (P). Soil samples were collected from arable lands, and the effects of the tested pollutants were compared with those used as control uncontaminated soil samples in terms of Zea mays biomass and the activity of seven enzymes in the soil. The following sorbents were applied to mitigate DO and P effects on the test plants and enzymatic activity: molecular sieve (M), expanded clay (E), sepiolite (S), and Ikasorb (I). Both DO and P exerted a toxic effect on Zea mays, with DO more strongly disturbing its growth and development and the activities of soil enzymes than P. In sandy clay (SL), P was found to be a significant inhibitor of dehydrogenases (Deh), catalase (Cat), urease (Ure), alkaline phosphatase (Pal), and arylsulfatase (Aryl) activities, while DO stimulated the activity of all enzymes in this soil. The study results suggest that the sorbents tested, mainlya molecular sieve, may be useful in remediating DO-polluted soils, especially when alleviating the effects of these pollutants in soils of lower agronomic value.

A non-canonical function of Arabidopsis ERECTA proteins and a role of the SWI3B subunit of the SWI/SNF chromatin remodeling complex in gibberellin signaling.

The Arabidopsis ERECTA family (ERf) of leucine-rich repeat receptor-like kinases (LRR-RLKs) comprising ERECTA (ER), ERECTA-LIKE 1 (ERL1), and ERECTA-LIKE 2 (ERL2) controls epidermal patterning, inflorescence architecture, and stomata development and patterning. These proteins are reported to be plasma membrane associated. Here we show that the er/erl1/erl2 mutant exhibits impaired gibberellin (GA) biosynthesis and perception alongside broad transcriptional changes. The ERf kinase domains were found to localize to the nucleus where they interact with the SWI3B subunit of the SWI/SNF chromatin remodeling complex (CRCs). The er/erl1/erl2 mutant exhibits reduced SWI3B protein level and affected nucleosomal chromatin structure. Similar to swi3c and brm plants with inactivated subunits of SWI/SNF CRCs, it also does not accumulate DELLA RGA and GAI proteins. The ER kinase phosphorylates SWI3B in vitro, and the inactivation of all ERf proteins leads to the decreased phosphorylation of SWI3B protein in vivo. The identified correlation between DELLA overaccumulation and SWI3B proteasomal degradation, and the physical interaction of SWI3B with DELLA proteins indicate an important role of SWI3B-containing SWI/SNF CRCs in gibberellin signaling. Co-localization of ER and SWI3B on GID1 (GIBBERELLIN INSENSITIVE DWARF 1) DELLA target gene promoter regions and abolished SWI3B binding to GID1 promoters in er/erl1/erl2 plants supports the conclusion that ERf-SWI/SNF CRC interaction is important for transcriptional control of GA receptors. Thus, the involvement of ERf proteins in the transcriptional control of gene expression, and observed similar features for human HER2 (epidermal growth family receptor member), indicate an exciting target for further studies of evolutionarily conserved non-canonical functions of eukaryotic membrane receptors.

Molecular Sieve, Halloysite, Sepiolite and Expanded Clay as a Tool in Reducing the Content of Trace Elements in Helianthus annuus L. on Copper-Contaminated Soil.

The aim of this study was to determine the effect of copper soil contamination on the trace element content of sunflower aerial parts and in roots. Another aim was to assess whether the introduction of selected neutralizing substances (molecular sieve, halloysite, sepiolite and expanded clay) into the soil could reduce the impact of copper on the chemical composition of sunflower plants. Copper soil contamination with 150 mg Cu2+ kg-1 of soil and 10 g of each adsorbent per kg of soil were used. Soil contamination with copper caused a significant increase in the content of this element in the aerial parts (by 37%) and roots (by 144%) of sunflower. Enriching the soil with the mineral substances reduced the amount of copper in the aerial parts of sunflower. Halloysite had the greatest effect (35%), while expanded clay had the smallest effect (10%). An opposite relationship was found in the roots of this plant. In copper-contaminated objects, a decrease in the content of cadmium and iron and an increase in the concentrations of nickel, lead and cobalt in the aerial parts and roots of sunflower were observed. The applied materials reduced the content of the remaining trace elements more strongly in the aerial organs than in the roots of sunflower. Molecular sieve had the greatest reducing effect on the content of trace elements in sunflower aerial organs, followed by sepiolite, while expanded clay had the least impact. The molecular sieve also reduced the content of iron, nickel, cadmium, chromium, zinc and, especially, manganese, whereas sepiolite reduced the content of zinc, iron, cobalt, manganese and chromium in sunflower aerial parts. Molecular sieve contributed to a slight increase in the content of cobalt, while sepiolite had the same effect on the content of nickel, lead and cadmium in the aerial parts of sunflower. All materials decreased the content of chromium in sunflower roots, molecular sieve-zinc, halloysite-manganese, and sepiolite-manganese and nickel. The materials used in the experiment, especially the molecular sieve and to a lesser extent sepiolite, can be used effectively to reduce the content of copper and some other trace elements, particularly in the aerial parts of sunflower.

The Impact of Permethrin and Cypermethrin on Plants, Soil Enzyme Activity, and Microbial Communities.

Pyrethroids are insecticides most commonly used for insect control to boost agricultural production. The aim of the present research was to determine the effect of permethrin and cypermethrin on cultured and non-cultivated bacteria and fungi and on the activity of soil enzymes, as well as to determine the usefulness of Zea mays in mitigating the adverse effects of the tested pyrethroids on the soil microbiome. The analyses were carried out in the samples of both soil not sown with any plant and soil sown with Zea mays. Permethrin and cypermethrin were found to stimulate the multiplication of cultured organotrophic bacteria (on average by 38.3%) and actinomycetes (on average by 80.2%), and to inhibit fungi growth (on average by 31.7%) and the enzymatic activity of the soil, reducing the soil biochemical fertility index (BA) by 27.7%. They also modified the number of operational taxonomic units (OTUs) of the Actinobacteria and Proteobacteria phyla and the Ascomycota and Basidiomycota phyla. The pressure of permethrin and cypermethrin was tolerated well by the bacteria Sphingomonas (clone 3214512, 1052559, 237613, 1048605) and Bacillus (clone New.ReferenceOTU111, 593219, 578257), and by the fungi Penicillium (SH1533734.08FU, SH1692798.08FU) and Trichocladium (SH1615601.08FU). Both insecticides disturbed the growth and yielding of Zea mays, as a result of which its yield and leaf greenness index decreased. The cultivation of Zea mays had a positive effect on both soil enzymes and soil microorganisms and mitigated the anomalies caused by the tested insecticides in the microbiome and activity of soil enzymes. Permethrin decreased the yield of its aerial parts by 37.9% and its roots by 33.9%, whereas respective decreases caused by cypermethrin reached 16.8% and 4.3%.

The development of recycling methods for bio-based materials - A challenge in the implementation of a circular economy: A review.

This review focuses on the characteristics of the most widely used biopolymers that contain starch, polylactic acid, cellulose and/or polybutylene succinate. Because worldwide production of bio-based materials has grown dynamically, their waste is increasingly found in the existing waste treatment plants. The development of recycling methods for bio-based materials remains a challenge in the implementation of a circular economy. This article summarizes the recycling methods for bio-based materials, which, in the hierarchy of waste management, is much more desirable than landfilling. Several methods of recycling are available for the end-of-life management of bio-based products, which include mechanical (reuse of waste as a valuable raw material for further processing), chemical (feedstock recycling) and organic (anaerobic digestion or composting) ones. The use of chemical or mechanical recycling is less favourable, more costly and requires the improvement of systems for separation of bio-based materials from the rest of the waste stream. Organic recycling can be a sustainable alternative to those two methods. In organic recycling, bio-based materials can be biologically treated under aerobic or anaerobic conditions, depending on the characteristics of the materials. The choice of the recycling method to be implemented depends on the economic situation and on the properties of the bio-based products and their susceptibility to degradation. Thus, it is necessary to label the products to indicate which method of recycling is most appropriate.

Multi-faceted analysis of thermophilic anaerobic biodegradation of poly(lactic acid)-based material.

Currently, the production of bio-based polymeric materials, of which poly(lactic acid) (PLA) is the most popular, has been increasing, causing the growth of PLA waste in municipal waste. Thus, it is necessary to develop sustainable methods for treating it. Methane production, resulting from anaerobic digestion (AD), is a potential end-of-life scenario for PLA waste that needs to be investigated. To obtain high efficiency of AD, thermophilic fermentation was applied, and to overcome low rates of biodegradation, hydrothermal (HT) and alkaline (A) pretreatments were used. For a deep insight into the process, differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FTIR), and microscopic and microbial analyses (based on 16S rDNA) were applied. For both untreated (PLA) and pretreated (PLAHT, PLAA) samples a high maximal methane production (MP) of 453 L/kg volatile solids (VS) was obtained, almost 100 % of the theoretical methane yield from PLA. The use of pretreatment allowed shortening of the time for obtaining maximal MP, especially the hydrothermal pretreatment, which shortened the overall time of MP 1.3-fold, and methane was produced at an almost 10 % higher rate (8.35 vs 7.79 L/(kg VS·d)). However, DSC and microscopic analyses revealed that, in all cases, methane was intensively produced i) after the reduction of the molecular mass of the PLA material and ii) also when PLA pieces were not visible. This should be considered when designing the operational time for the AD process. Parallel to the gradual biodegradation of PLA, the abundances of Firmicutes, Thermotogae, and Euryarcheota increased. With PLAHT, Syntrophobacteraceae, Thermoanaerobacteraceae, and methanogens were identified as potential key thermophilic PLA biodegraders.

Versatile Polypropylene Composite Containing Post-Printing Waste.

The paper presents the results of the research on the possibility of using waste after the printing process as a filler for polymeric materials. Remains of the label backing were used, consisting mainly of cellulose with glue and polymer label residue. The properly prepared filler (washed, dried, pressed and cut) was added to the polypropylene in a volume ratio of 2:1; 1:1; 1:2; and 1:3 which corresponded to approximately 10, 5, 2.5 and 2 wt % filler. The selected processing properties (mass flow rate), mechanical properties (tensile strength, impact strength, dynamic mechanical analysis) and thermal properties (thermogravimetric analysis, differential scanning calorimetry) were determined. The use of even the largest amount of filler did not cause disqualifying changes in the determined properties. The characteristics of the obtained materials allow them to be used in various applications while reducing costs due to the high content of cheap filler.

Plastic Waste Degradation in Landfill Conditions: The Problem with Microplastics, and Their Direct and Indirect Environmental Effects.

As landfilling is a common method for utilizing plastic waste at its end-of-life, it is important to present knowledge about the environmental and technical complications encountered during plastic disposal, and the formation and spread of microplastics (MPs) from landfills, to better understand the direct and indirect effects of MPs on pollution. Plastic waste around active and former landfills remains a source of MPs. The landfill output consists of leachate and gases created by combined biological, chemical, and physical processes. Thus, small particles and/or fibers, including MPs, are transported to the surroundings by air and by leachate. In this study, a special focus was given to the potential for the migration and release of toxic substances as the aging of plastic debris leads to the release of harmful volatile organic compounds via oxidative photodegradation. MPs are generally seen as the key vehicles and accumulators of non-biodegradable pollutants. Because of their small size, MPs are quickly transported over long distances throughout their surroundings. With large specific surface areas, they have the ability to absorb pollutants, and plastic monomers and additives can be leached out of MPs; thus, they can act as both vectors and carriers of pollutants in the environment.

Evaluation of the Usefulness of Sorbents in the Remediation of Soil Exposed to the Pressure of Cadmium and Cobalt.

An undesirable side effect of economic progress is increasingly severe pollution with heavy metals, responsible for the degradation of ecosystems, including soil resources. Hence, this research focused on examining six adsorbents in order to distinguish a reactive mineral with the highest capacity to remediate soils contaminated with heavy metals. To this end, the soil was polluted with Co2+ and Cd2+ by applying the metals in concentrations of 100 mg kg-1 d.m. The extent of soil equilibrium disturbances was assessed by evaluating the response of the soil microbiome, activity of seven soil enzymes, and the yields of Helianthus annuus L. Six sorbents were evaluated: a molecular sieve, expanded clay (ExClay), halloysite, zeolite, sepiolite and biochar. Co2+ and Cd2+ proved to be significant inhibitors of the soil's microbiological and biochemical parameters. Organotrophic bacteria among the analysed groups of microorganisms and dehydrogenases among the soil enzymes were most sensitive to the effects of the metals. Both metals significantly distorted the growth and development of sunflower, with Co2+ having a stronger adverse impact on the synthesis of chlorophyll. The molecular sieve and biochar were the sorbents that stimulated the multiplication of microorganisms and enzymatic activity in the contaminated soil. The activity of enzymes was also stimulated significantly by zeolite and sepiolite, while the growth of Helianthus annuus L. biomass was stimulated by the molecular sieve, which can all be considered the most useful reactive materials in the remediation of soils exposed to Co2+ and Cd2+.

Mitigation of the Adverse Impact of Copper, Nickel, and Zinc on Soil Microorganisms and Enzymes by Mineral Sorbents.

Despite numerous studies on the influence of heavy metals on soil health, the search for effective, eco-friendly, and economically viable remediation substances is far from over. This encouraged us to carry out a study under strictly controlled conditions to test the effects of Cu2+, Ni2+, and Zn2+ added to soil in amounts of 150 mg·kg-1 d.m. of soil on the soil microbiome, on the activity of two oxidoreductases and five hydrolases, and on the growth and development of the sunflower Helianthus annunus L. The remediation substances were a molecular sieve, halloysite, sepiolite, expanded clay, zeolite, and biochar. It has been demonstrated that the most severe turbulences in the soil microbiome, its activity, and the growth of Helianthus annunus L. were caused by Ni2+, followed by Cu2+, and the mildest negative effect was produced by Zn2+. The adverse impact of heavy metals on the soil microbiome and its activity was alleviated by the applied sorbents. Their application also contributed to the increased biomass of plants, which is significant for the successful phytoextraction of these metals from soil. Irrespective of which property was analysed, sepiolite can be recommended for the remediation of soil polluted with Ni2+ and zeolite-for soil polluted with Cu2+ and Zn2+. Both sorbents mitigated to the highest degree disturbances caused by the tested metals in the soil environment.

Effect of Separate and Combined Toxicity of Bisphenol A and Zinc on the Soil Microbiome.

The research objective was established by taking into account common sources of soil contamination with bisphenol A (B) and zinc (Zn2+), as well as the scarcity of data on the effect of metabolic pathways involved in the degradation of organic compounds on the complexation of zinc in soil. Therefore, the aim of this study was to determine the spectrum of soil homeostasis disorders arising under the pressure of both the separate and combined toxicity of bisphenol A and Zn2+. With a broad pool of indicators, such as indices of the effect of xenobiotics (IFX), humic acid (IFH), plants (IFP), colony development (CD), ecophysiological diversity (EP), the Shannon-Weaver and the Simpson indices, as well as the index of soil biological fertility (BA21), the extent of disturbances was verified on the basis of enzymatic activity, microbiological activity, and structural diversity of the soil microbiome. A holistic character of the study was achieved, having determined the indicators of tolerance (IT) of Sorghum Moench (S) and Panicum virgatum (P), the ratio of the mass of their aerial parts to roots (PR), and the SPAD leaf greenness index. Bisphenol A not only failed to perform a complexing role towards Zn2+, but in combination with this heavy metal, had a particularly negative effect on the soil microbiome and enzymatic activity. The NGS analysis distinguished certain unique genera of bacteria in all objects, representing the phyla Actinobacteriota and Proteobacteria, as well as fungi classified as members of the phyla Ascomycota and Basidiomycota. Sorghum Moench (S) proved to be more sensitive to the xenobiotics than Panicum virgatum (P).

Strategies of Recovery and Organic Recycling Used in Textile Waste Management.

Post-consumer bio-based textile wastes are any type of garment or household article made from manufactured bio-based textiles that the owner no longer needs and decides to discard. According to the hierarchy of waste management, post-consumer textile waste should be organically recycled. However, there is still a problem with the implementation of selective collection of textile waste followed by sorting, which would prepare the waste for organic recycling. A technically achievable strategy for sorted textile waste materials consisting of only one type of fiber material, multi-material textiles are a problem for recycling purposes. Waste textiles are composed of different materials, including natural as well as synthetic non-cellulosic fibers, making bioprocessing difficult. Various strategies for recovery of valuable polymers or monomers from textile waste, including concentrated and dilute acid hydrolysis, ionic liquids as well as enzymatic hydrolysis, have been discussed. One possible process for fiber recycling is fiber recovery. Fiber reclamation is extraction of fibers from textile waste and their reuse. To ensure that organic recycling is effective and that the degradation products of textile waste do not limit the quality and quantity of organic recycling products, bio-based textile waste should be biodegradable and compostable. Although waste textiles comprising a synthetic polymers fractions are considered a threat to the environment. However, their biodegradable part has great potential for production of biological products (e.g., ethanol and biogas, enzyme synthesis). A bio-based textile waste management system should promote the development and application of novel recycling techniques, such as further development of biochemical recycling processes and the textile waste should be preceded by recovery of non-biodegradable polymers to avoid contaminating the bioproducts with nano and microplastics.

Sensitivity of Zea mays and Soil Microorganisms to the Toxic Effect of Chromium (VI).

Chromium is used in many settings, and hence, it can easily enter the natural environment. It exists in several oxidation states. In soil, depending on its oxidation-reduction potential, it can occur in bivalent, trivalent or hexavalent forms. Hexavalent chromium compounds are cancerogenic to humans. The aim of this study was to determine the effect of Cr(VI) on the structure of bacteria and fungi in soil, to find out how this effect is modified by humic acids and to determine the response of Zea mays to this form of chromium. A pot experiment was conducted to answer the above questions. Zea mays was sown in natural soil and soil polluted with Cr(VI) in an amount of 60 mg kg-1 d.m. Both soils were treated with humic acids in the form of HumiAgra preparation. The ecophysiological and genetic diversity of bacteria and fungi was assayed in soil under maize (not sown with Zea mays). In addition, the following were determined: yield of maize, greenness index, index of tolerance to chromium, translocation index and accumulation of chromium in the plant. It has been determined that Cr(VI) significantly distorts the growth and development of Zea mays, while humic acids completely neutralize its toxic effect on the plant. This element had an adverse effect on the development of bacteria of the genera Cellulosimicrobium, Kaistobacter, Rhodanobacter, Rhodoplanes and Nocardioides and fungi of the genera Chaetomium and Humicola. Soil contamination with Cr(VI) significantly diminished the genetic diversity and richness of bacteria and the ecophysiological diversity of fungi. The negative impact of Cr(VI) on the diversity of bacteria and fungi was mollified by Zea mays and the application of humic acids.

Bisphenol A-A Dangerous Pollutant Distorting the Biological Properties of Soil.

Bisphenol A (BPA), with its wide array of products and applications, is currently one of the most commonly produced chemicals in the world. A narrow pool of data on BPA-microorganism-plant interaction mechanisms has stimulated the following research, the aim of which has been to determine the response of the soil microbiome and crop plants, as well as the activity of soil enzymes exposed to BPA pressure. A range of disturbances was assessed, based on the activity of seven soil enzymes, an abundance of five groups of microorganisms, and the structural diversity of the soil microbiome. The condition of the soil was verified by determining the values of the indices: colony development (CD), ecophysiological diversity (EP), the Shannon-Weaver index, and the Simpson index, tolerance of soil enzymes, microorganisms and plants (TIBPA), biochemical soil fertility (BA21), the ratio of the mass of aerial parts to the mass of plant roots (PR), and the leaf greenness index: Soil and Plant Analysis Development (SPAD). The data brought into sharp focus the adverse effects of BPA on the abundance and ecophysiological diversity of fungi. A change in the structural composition of bacteria was noted. Bisphenol A had a more beneficial effect on the Proteobacteria than on bacteria from the phyla Actinobacteria or Bacteroidetes. The microbiome of the soil exposed to BPA was numerously represented by bacteria from the genus Sphingomonas. In this object pool, the highest fungal OTU richness was achieved by the genus Penicillium, a representative of the phylum Ascomycota. A dose of 1000 mg BPA kg-1 d.m. of soil depressed the activity of dehydrogenases, urease, acid phosphatase and ß-glucosidase, while increasing that of alkaline phosphatase and arylsulfatase. Spring oilseed rape and maize responded significantly negatively to the soil contamination with BPA.

Perna canaliculus as an Ecological Material in the Removal of o-Cresol Pollutants from Soil.

Soil contamination with cresol is a problem of the 21st century and poses a threat to soil microorganisms, humans, animals, and plants. The lack of precise data on the potential toxicity of o-cresol in soil microbiome and biochemical activity, as well as the search for effective remediation methods, inspired the aim of this study. Soil is subjected to four levels of contamination with o-cresol: 0, 0.1, 1, 10, and 50 mg o-cresol kg-1 dry matter (DM) of soil and the following are determined: the count of eight groups of microorganisms, colony development index (CD) and ecophysiological diversity index (EP) for organotrophic bacteria, actinobacteria and fungi, and the bacterial genetic diversity. Moreover, the responses of seven soil enzymes are investigated. Perna canaliculus is a recognized biosorbent of organic pollutants. Therefore, microbial biostimulation with Perna canaliculus shells is used to eliminate the negative effect of the phenolic compound on the soil microbiome. Fungi appears to be the microorganisms most sensitive to o-cresol, while Pseudomonas sp. is the least sensitive. In o-cresol-contaminated soils, the microbiome is represented mainly by the bacteria of the Proteobacteria and Firmicutes phyla. Acid phosphatase, alkaline phosphatase and urease can be regarded as sensitive indicators of soil disturbance. Perna canaliculus shells prove to be an effective biostimulator of soil under pressure with o-cresol.

Immunomodulatory effects exerted by extracellular vesicles from Staphylococcus epidermidis and Staphylococcus aureus isolated from bone-anchored prostheses.

Staphylococcus aureus and Staphylococcus epidermidis are the bacteria that most frequently cause osteomyelitis. This study aimed to determine whether staphylococci isolated from osteomyelitis associated with septic loosening of orthopedic prostheses release extracellular vesicles (EVs) and, if so, to determine tentative immunomodulatory effects on the human monocytic cell line THP-1. EVs were isolated from bacterial cultures using filtration and ultracentrifugation and characterized by scanning electron microscopy, nanoparticle tracking analysis and Western Blot. The cytotoxic effect of EVs was analyzed by NucleoCounter and lactate dehydrogenase (LDH) analyses. Confocal laser scanning microscopy was employed to visualize the uptake of EVs by THP-1 cells. Activation of the transcription factor nuclear factor-κB (NF-κB) was determined in THP1-Blue™ NF-κB cells, and the gene expression and secretion of cytokines were determined by quantitative polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. All investigated strains, irrespective of their biofilm formation ability, were able to secrete EVs in vitro. The S. aureus strains produced significantly more EVs than the S. epidermidis strains. Both S. aureus-derived EVs and S. epidermidis-derived EVs were internalized by THP-1 cells, upregulated Toll-like receptor 3 (TLR3) gene expression, activated NF-κB, and promoted the gene expression and secretion of interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, matrix metallopeptidase (MMP)-9 and IL-10. Whereas EVs from both staphylococcal species upregulated the proapoptotic DNA damage-inducible transcript 4 (DDIT4) gene and downregulated the antiapoptotic B-cell lymphoma 2 (Bcl-2) gene, cytolysis was preferentially induced in S. aureus EV-stimulated cells, possibly related to the expression of cytolytic proteins predominantly in S. aureus EVs. In conclusion, staphylococcal EVs possess potent cytolytic and immunomodulatory properties.