RESUMO
During production of radiopharmaceuticals, the radiation situation in cyclotron pit is an important parameter, which is being monitored to ensure fulfilment of the limits and conditions of safe operation. The neutron flux in the structural components of the accelerator is also an important parameter, because the secondary neutrons are responsible for activation of cyclotron structural components and may even affect structural changes in it. This paper aims to characterize the neutron field in inner positions of medical accelerator IBA 18/9 by activation detectors and by means of scintillation spectrometry. The backward angle measurement was realized also in special liquid water target (H218O) at U120M cyclotron to confirm the data obtained in IBA 18/9 cyclotron.
RESUMO
Clustered DNA damage induced by 10, 20 and 30 MeV protons in pBR322 plasmid DNA was investigated. Besides determination of strand breaks, additional lesions were detected using base excision repair enzymes. The plasmid was irradiated in dry form, where indirect radiation effects were almost fully suppressed, and in water solution containing only minimal residual radical scavenger. Simultaneous irradiation of the plasmid DNA in the dry form and in the solution demonstrated the contribution of the indirect effect as prevalent. The damage composition slightly differed when comparing the results for liquid and dry samples. The obtained data were also subjected to analysis concerning different methodological approaches, particularly the influence of irradiation geometry, models used for calculation of strand break yields and interpretation of the strand breaks detected with the enzymes. It was shown that these parameters strongly affect the results.
