Assuntos
Dor Abdominal/etiologia , Adolescente , Adulto , Criança , Pré-Escolar , Humanos , RecidivaRESUMO
In 1984-1985 72 specimens of human milk were examined for hexachlorobenzene, DDE and polychlorinated biphenyls and 69 specimens for lead and cadmium. The residual levels (expressed in mg.kg-1 milk fat) were as follows: hexachlorobenzene 1.97 (0.24-5.58), DDE 1.56 (0.17-5.80) and polychlorinated biphenyls 3.18 (1.28-9.08). All specimens were positive. The lead levels were 1.68 (less than 0.10-6.75), cadmium levels 0.31 (less than 0.05-1.08) ng.ml-1 milk. Lead was present in 94.2%, cadmium in 98.6% specimens. The results were compared with other areas of the CSSR, data from the literature and hygienic standards. The authors discuss limited or discontinued breastfeeding in high occupational exposure. Finally they emphasize advantages associated with breastfeeding and the justification of a prolactation programme.
Assuntos
Poluentes Ambientais/análise , Leite Humano/análise , Cádmio/análise , Humanos , Chumbo/análise , Resíduos de Praguicidas/análiseRESUMO
We describe a method for determining serum alkaline phosphatase activity with use of N-methyl-D-glucamine buffer, Na+ is a definite activator, whereas NH4+ and Li+ inhibit enzyme activity. Optimum reaction conditions are: methylglucamine buffer, 0.35 mol/L, pH 10.2 +/- 0.1 (30 degrees C); NaCl, 70 mmol/L; MgCl2, 0.5 mmol/L; disodium 4-nitrophenyl phosphate, 15 mmol/L; reaction temperature, 30 degrees C; reaction time, 2 min. The assay conditions are optimum for all human serum isoenzymes.