[Use of PCR for detection of maize seeds infected with the fungus Fusarium moniliforme Sheldon var.lactis]. / Ispol'zovanie polimeraznoi tsepnoi reaktsii dlia detektsii zagriazneniia zerna kukuruzy gribom Fusarium moniliforme Sheldon.

The procedure of the PCR for the detection of Fusarium moniliforme Sheldon var. lactis on the maize seeds has been elaborated. The based on a highly sensitive molecular genetics technology method of fungal pathogen detection allows to reveal its presence before the visual symptoms of seed damage.

[Increased selectivity of directed RNA hydrolysis mediated by RNAse H from E. coli using mixed oligo(deoxyribo-ribo)nucleotides]. / Povyshenie izbiratel'nosti napravlennogo gidroliza RNK RNKazoi H iz Ecoli s pomoshch'iu smeshannykh oligo(dezoksiribo-ribo)-nukleotidov.

Chimeric oligo(deoxyribo-ribo)nucleotides appeared to be a valuable tool to achieve the high selectivity of RNA cleavage as shown by RNA-ase H-mediated hydrolysis of TMV RNA directed by d(TGTGTATGCC), d(TGTGTAT), d(TGTGTAT)GCCAU and d(TGTGTAT)ppGCCAU.

[Chemico-enzymatic method of synthesis of mixed oligonucleotides]. / Khimiko-fermentativnyi metod sinteza smeshannykh oligonukleotidov.

Oligo (ribodeoxyribo)nucleotide d(TGTGTAT)r(GCCAU) was synthesized from oligonucleotide blocks by chemical ligation, the oligoribonucleotide and oligodeoxynucleotide blocks being prepared by enzymatic and chemical synthesis, resp. A general procedure of the hybride duplex strand separation is described.