[Control of fascioliasis in heifers in Czechoslovakia 1970-1985]. / Tlumení fasciolózy u skotu v CSR v letech 1970 az 1985.

A programme for the control of fascioliasis caused by F. hepatica in cattle was launched in 1970 and continued until 1985. The animals were subjected to helminth-control treatment and preventive measures were taken in the herds. The anti-fasciolics Bilevon M and to some extent also Bromsalan pulv. were administered by the veterinary service on the basis of state subsidies in 1973 to 1979. The preparation Helmisan (Spofa) has been in use since 1980; its production is subsidized by the agricultural enterprises. In the period from 1972 ot 1979, up to 213 thousand helminth-control treatments (both therapeutic and preventive) were performed every year in the cattle. All treatments reduced the occurrence of Fasciola hepatica and pathological changes in the liver of the killed cattle (from 5.8% recorded in 1971 to 0.9 in 1980, on an national average). As a result, 29 districts are free from fascioliasis although the disease originally occurred in all districts of the Czech Socialist Republic. After ten years of purposeful use of antifasciolics, the findings were free from living flukes and the cattle only showed pathological changes after the action of F. hepatica. Since 1980 the extensity of the findings of F. hepatica in slaughtered cattle has ranged within one percent, though living flukes have recently occurred again in the liver of certain animals. In the enzootically affected regions and districts of the Czech Socialist Republic the fascioliasis control programme succeeded in reducing the number of invaded cattle herds.

[The incidence of coccidia of the genus Cryptosporidium (Cryptosporidiidae) in diarrheal diseases in calves]. / Výskyt kokcidií rodu Cryptosporidium (Cryptosporidiidae) pri prujmových onemocneních telat.

In the period from March 1983 to June 1984, the occurrence of Cryptosporidium sp. coccidia in smears of gut contents and samples of excrements stained after Heine (1982) was investigated in calves at the age of 30 days, coming from 16 farms of central Bohemia. The oocysts of Cryptosporidium sp. were diagnosed in 68 calves (occurrence extensity 37.15%) out of the 183 dissected calves with the indication of digestion disorders and in 177 samples of excrements (extensity 33.71%) out of the 525 samples examined. The maximum occurrence of cryptosporidia was recorded in ten- and fourteen-day-old calves (13.9-11.3%); the first findings were reported on the third day of the age of the calves. The intensity of infections induced by cryptosporidia, evaluated as medium-strong, was reported in 33.82% of the dissected calves, in 50% of the samples of excrements of the rearing halls of large cow-houses, and in 36.52% of the samples from large calf-houses. The largest findings of cryptosporidia were associated with the diagnosis of acute catarrhal enteritis (12.02%), catarrhal haemorrhagic enteritis or catarrhal haemorrhagic ileitis (5.46%); however, they were also found when the finding in the digestive tract was negative. Simultaneous bacteriological finding of Escherichia coli and Cryptosporidium sp. occurred at acute catarrhal enteritis (19.12%) and catarrhal haemorrhagic enteritis (11.47%); a simultaneous finding of Salmonella sp. was reported in calves suffering from acute catarrhal enteritis (3.27%). In the calves the occurrence of cryptosporidia was not accompanied by parallel bacteriological findings (4.37%).

[Developmental stages of Taenia krabbei Moniez, 1879 (Cysticercus tarandi) in deer (Capreolus capreolus L.) in Czechoslovakia]. / Vývpkpvá stadia Taenia krabbei Moniez, 1879 (Cysticercus tarandi) u srncí zvére (Capreolus capreolus L.) v CSR.

The breast and shoulder-blade muscles of a roe (Caprelous capreolus L.), shot in a hunting district in western Bohemia, were found to contain cysticerci with morphological characters and size corresponding to those of Cysticercus tarandi, which is a developmental stage of the tapeworm Taenia krabbei Moniez, 1879. The surface as well as deeper layers of muscle contained one to two cysticerci per 100 cm2 of area. The authors describe the larvocysts and histological changes in the site where the micro-organisms were found. The cysticerci are discussed as to the species to which they belong.

[Efficacy of mebendazole in sheep experimentally invaded with larvae of Haemonchus contortus and Trichostrongylus colubriformis in relation to the results of clinical examination]. / Ucinnost mebendazolu u ovcí experimentálne invadovaných larvami Haemonchus contortus a Trichostrongylus colubriformis ve vztahu k vysledkúm klinického vysetrení.

A group of six lambs at the age of two months and of the average weight of 13 kg were invaded with the larvae Haemonchus contortus and Trichostrongylus colubriformis; the course of invasion was compared with that in a group of four control lambs. In regular ten-day intervals, some hematological indices, proteinemia. SGOT and SGPT activities, glucose level, sodium and potassium were studied in blood samples; at the same time egg elimination was observed in excrement samples. The invasion intensity was checked by helminthological dissection of an experimentally invaded lamb 35th day p.i.; 576 H. contortus adults and 398 T. colubriformis adults were determined. Egg number recalculated per 1 g excrements (E.P.G.) amounted to 3072. At that stage, both groups were administered mebendazole at a dose of 10 mg active substance per 1 kg live weight on three successive days. The efficacy of the treatment (IE) was 97.01 to 98.05%. According to the clinical examination the hematocrit level dropped statistically significantly on the ninth day p.i., the other indices under study showed no changes. The drug administration increased temporarily the erythrocyte count and decreased MCV and MCH. Although mebendazole mainly inhibited glucose metabolism in the nematodes, the drug administration did not influence the glucose level of the host's blood serum.

[The effect of dehelminthizations performed during the year on the seasonal dynamics of natural nematode infections in sheep]. / Vliv dehelmintizací uskutechených v prubehu roku na sezónní dynamiki prirozených invazí nematodu u ovcí

In four sheep flocks of two age categories dynamics of natural infections by pulmonary and gastrointestinal nematodes was studied; sheep were kept on a farm in Western Bohemia. Dehelminthizations were performed in different intervals during the grazing period on the basis of the results of quantitative coprologic examinations. Total effectiveness of 80--100% intenseffectiveness (IE) was obtained as a result of single peroral or intraruminal dehelminthization with the following preparations: pyrantel hydrochloricum (Spofa), helmatac (SKF) and nilverm (ICI); the effectiveness concerned gastrointestinal nematodes of the genus Haemonchus, Cooperia, Ostertagia, Trichostrongylus, Bunostomum, Chabertia, Nematodirus, Strongyloides, Oesophagostomum and Trichocephalus. The effectiveness of nilverm on lungworms of D. filaria and P. kochi reached 100%; the preparation was less effective and ineffective on M. capillaris. Dehelminthization practices during three years were more successful as to lowering of incidence of lungworm infections of D. filaria and P. kochi than in gastrointestinal nematodes. If sping dehelminthizations had been postponed till the second half of May or June, the climax of the elimination of ova from summer re-infection was put off till November, with an initial significant increase in September. The third dehelminthization, applied in August, did not result in the increased elimination of ova in autumn, while there was no usual autumnal climax following September dehelminthization. Effective dehelminthization performed at the end of November in all three years maintained low levels of infections during winter housing and significantly influenced the health conditions of ewes before lambing. Dynamics of the elimination of ova after dehelminthization was affected by nematodes with the migration phase in organs and tissues -- S. papillosus, Oesophagostomum sp. and Ostertagia sp.; the same effect was observed, during pasture, in nematodes with relatively short exogenous development of the genus Haemonchus, Ostertagia, Trichostrongylus and Nematodirus. In spite of these dehelminthization practices, the elimination of ova and larvae corresponded to the course of temperatures and rainfall in spring and autumn climaxes every year.

[Clinical examination of the blood of red deer (Cervus elaphus L.) naturally infested with parasites]. / Klinick e vysetrení krve jelení zvere (Cervus elaphus L.) Prirozene invadované cizopasníky

In six animals hunted and four immobilized animals of red deer (Cervus elaphus L.) of both sexes and of different age, kept at three game preserves in Bohemia, the psychological values were ascertained: the number of erythrocytes and leucocytes, the hemoglobin content, the hematocrit, MCV, MCH, MCHC, the white blood count, the total serum protein, the fractions: albumin, alpha-, beta- and gamma-globulin, the activity of SGOT, and SGPT, the alkaline phosphatase and the metabolism of calcium, phosphorus, and magnesium in the blood serum. The blood from the heart shortly after killing of the animal was examined, and in immobilized animals the blood was taken from the vena jugularis. In the red deer, a variable intensity of the polyvalent infection of parasites of the species Dictyocaulus viviparus, Bicalulus sagittatus, Fascioloides magna, Paramphistomum spec., Haemonchus contortus, Ostertagia ostertagi, O. circumcincta, Trichostrongylus axei, Nematodirus filicollis, Chabertia ovina, and Trichocephalus globulosa was found. In the immobilized deer no marked deviations were found in the examined values of blood that had been taken within 10 minutes after the calming of the animals.

[Clinical examination of the blood of wild boars (Sus scrofa L.) naturally infested with parasites following administration of anthelminthics]. / Klinické vysetrení drve cerné zvere (Sus scrofa l.) priprozene invadované parazity po aplikaci anthelmintik

With 24 head of wild boars (Sus scrofa L.) of both sexes of the age of one to two years, kept in a game preserve and naturally infected with parasites of the species Mestastrongylus pudendotectus, M. elongatus, Ascarops strongylina, Physocephalus sexalatus, Ascaris suum, Globocephalus urosubulatus, Oesophagostomum dentatum, Trichocephalus suis, Eimeria debliecki, and E. perminuta, three experiments were performed with feeds premedicated with anthelmintics: pyrantel tartrate + diethylcarbamazine tartrate (a dose of 25 + 50 mg kg-1 of live weight), Mebendazole 5 p. c. premix and Mebendazole 50 p. c. premix (doses of 10 mg kg-1 and 40 mg kg-1 of live weight) administered for three consecutive days. According to the results of the helminthological dissections the effectiveness of pyrantel tartrate + diethylcarbamazine tartrate reached from 58.1 to 100 p. c. compared with the different species of nematodes, Mebendazole 5 p.c. premix from 47.8 to 100 p. c., and Mebendazole 50 p. c. premix reached the effectiveness of 85.4 to 100 p.c with the mentioned therapeutic doses. The results obtained from a coprological investigation showed the effectiveness, in the case of pyrantel tartrate + diethylcarbamazine tartrate, of 99.2 and 70.65 p. c. on the sixth and fifteeenth day after application, in the case of Mebendazole 5 p. c. premix 94.4 and 79.41 p. c. on the tenth and twentieth day after application, and in the case of of Mebendazole 50 p. c. premix it amounted to 96.0 and 100 p. c. on the tenth and twentieth day after application. In 22 head of wild boars of the total number of examined animals the minimum, maximum, and average values of the number of erythrocytes, leucocytes, of the hemoglobin content, of the hematocrit, MCV, MCH, MCHC, of the white blood count, of the protein total, of the fractions of albumin, alpha-, beta-, and gamma-globulin, of the activity of SGOT and SGPT, and of the metabolism of calcium, phosphorus, and magnesium were determined. The investigation was performed in the shortest possible time after the killing of the animals. In the examined values no marked deviations in 48 hours were found after the application had finished compared with the values determined in non-treated animals.

[Clinical examination of the blood of roe deer (Capreolus capreolus L.) and fallow deer (Dama dama L.) naturally invaded by parasites]. / Klinické vysetrení krve srnci zvere (Capreolus capreolus L.) a dancí zvere (Dama dama L.) prirozene invadované parazity

Roe-deer (Capreolus capreolus L. -- five animals) and fallow deer (Dama dama L. -- eleven animals) of both sexes and at different age were subject to blood examination. The deer were killed in several preserves in Bohemia during autumn and winter shootings in two years. The following blood values were determined: erythrocyte count, leucocyte count, hemoglobin content, hematocrit values, MCV, MCH, MCHC, white blood picture, total protein, its fractions (albumin, alpha-, beta-, and gamma-globulin) SGOT, SGPT, and alkaline phosphatase activities, and calcium, phoshporus, and magnesium levels. Examinations were performed in the blood collected from heart soon after the killing of the animals and the results were evaluated in relation to natural polyvalent invasions by parasites of the following species: Bicaulus sagittatus, Dictyocaulus viviparus, Paramphistomum sp., Haemonchus contortus, Ostertagia circumcincta, O. ostertagi, Trichostrongylus colubriformis, Bunostomum trigonocephalum, Nematodirus filicollis, Chabertia ovina, Oesophagostomum columbianum, Trichocephalus ovis, Eimeria auburnensis, E. faurei, and E. ninaekohlyakimovae, occurring in different intensities and species composition in individual animals of the deer tested.