[Pathogenesis of enteral insufficiency in patients with postoperative peritonitis].

Enteral insufficiency is considered to trigger the syndrome of endogenous intoxication and, further, multiple organ failure. Enteral insufficiency often accompanies postoperative septic complications, such as peritonitis. Morphologic changes of the intestinal wall by septic peritonitis consisted of edema, fibrinous degradation of muscular layer and dystrophy of local nervous pathways. Microbiologic analysis of intestinal and gastric contents, peritoneal fluid and blood samples demonstrated a broad spectrum of pathogenic flora. Besides, enterotoxin allocated from blood and intestinal contents coincided in all cases. Adequate algorithm of detoxication, including extracorporal methods, had been worked out.

[Chemiluminescence of whole saliva in antioxidant treatment of prosthetic bed tissues].

Inflammatory reaction is always accompanied by increased intensity of free-radical oxidation, especially when the phenomena of hypoxia and microcirculatory disorders that occur during the development of side-effects of acrylic removable dentures. This study determined the effectiveness of adaptogens, antioxidants in the complex treatment of diseases of tissues prosthetic field and their influence on the processes of LPO in whole mixed unstimulated saliva. Formed in the reaction to initiate the process of oxygen radicals (OH, RO, O(2)), initiate the formation of lipid peroxide radicals RO(2) biological substrate, the recombination of which leads to the emergence of unsustainable tetroxids, which decays with the release of light quanta. This luminescence is recorded as an amplified current of the photomultiplier, the registration systems. The results suggest the intensive formation of free radicals and peroxides in diseased tissue prosthetic field. Probably the main reason for increasing free-radical oxidation is the release of peroxidase from the crumbling inflammation, phagocytes (mainly neutrophils). The process of peroxidation contributes to an increase in blood supply to inflamed tissues, leading to local enrichment of oxygen, as well as toxic effects of acrylic bases of partial and complete removable dentures in the prosthetic field of tissue. Effect of antioxidants in combination with traditional treatment in 70 patients with periodontal disease and prosthetic bed was assessed by chemiluminescence analysis of whole mixed unstimulated saliva. The level of lipid peroxidation and chemiluminescence activity exceeded the normal values in the 1,5-2 - twice before the treatment. After treatment with antioxidants, these parameters decreased and increased during remission. Thus, studies to determine the status of saliva chemiluminescence method to treat and monitor the dynamics after treatment of periodontitis tissues supporting teeth prosthetic field in the control group and the main observation, revealed the following pattern: the approach of all the indices to normal in patients with a core group, which corresponded to the clinical dynamic index parameters of periodontal tissues supporting teeth prosthetic field, and a similar core group of the positive dynamics of the intensity values of chemiluminescence-indicators in the control group up to 3 months of observation, with a significant deterioration of the same indicators at a later time dynamic monitoring.

[Monovalent ion permeability of model bilayer membranes based on lipids from tissues of various vertebrates]. / Ionnaia pronitsaemost' model'nykh bisloinykh membran iz lipidov tkanei razlichnykh pozvonochnykh dlia odnovalentnykh ionov.

The ionic permeability of model bilayer membranes prepared from total lipids of the brain, heart, liver, and muscles of four species of higher vertebrates was studies. The electrical conductivity and potential of membranes breakdown were measured in KCl, NaCl, and LiCl solutions. It was found that the permeability for potassium ions of bilayer lipid membranes from the lipids of nervous tissue decreases in the row of poikilothermal vertebrates and increases in mammals. A reverse regularity was observed for membrane stability, which manifested itself as an increase in membrane breakdown in the order fish-amphibian-reptile and a decrease in membrane breakdown in mammalian membranes. It was shown that, in most cases, the the permeability of bilayer lipid membranes for K+ ions is higher than for Na+ and Li+.

Evidence of oxidative stress in erythrocyte phospholipid composition in the pathogenesis of familial Mediterranean fever (periodical disease).

BACKGROUND: Familial Mediterranean fever (FMF) is a genetically linked disorder common amongst races of the Eastern Mediterranean region. Typical symptoms include episodic pain syndrome extending throughout the chest or abdomen associated with histopathological signs of amyloidosis of the kidney. AIM: To investigate possible connections between the aseptic inflammation that occurs during pain crises and cell membrane structural and functional integrity in patients with FMF. METHODS: Oxidative stress parameters in 42 patients in remission and during a pain crisis were compared with 21 normal subjects. RESULTS: The patient group had significantly greater concentrations of chemiluminescent and thiobarbituric acid-reactive substances in the blood plasma and lower concentrations of alpha-tocopherol than the control group while in remission; these changes were exacerbated during pain crises. Analyses of the phospholipid composition of erythrocyte membranes showed significant increases in amounts of acidic phospholipids (phosphatidic acid, monophosphatidylinositol and cardiolipin) and lysophosphatidylcholine compared with healthy subjects. CONCLUSIONS: The pattern of differences in membrane phospholipid composition was consistent with increased oxidative stress in patients with FMF.

[Kinetics of lipid peroxidation induced by Fe2+ ions in a suspension of mitochondrial and nuclear membranes]. / Kinetika perekisnogo okisleniia lipidov, indutsirovannogo ionami Fe2+ v suspenzii mitokhondrial'nykh i iadernykh membran.

Kinetics of TBARS accumulation, Fe2+ oxidation, and chemiluminescence were measured in nuclear and mitochondrial membrane suspensions during Fe(2+)-induced lipid peroxidation development. Above certain critical Fe2+ concentrations ([Fe2+].) the latent period (t) of lipid peroxidation development were observed. By using the dependence of t on [Fe2+] the [Fe2+]. values were measured in nuclear and mitochondrial membrane suspensions. At the same concentrations of nuclear and mitochondrial membranes (1 mg/ml) the [Fe2+] value changed from 10 to 30 mu kM. In the case of nuclear membrane suspension the [Fe2+] value changed from 20 to 30 mu kM with the change of membrane concentrations from 0.5 to 1.5 mg/ml. For mitochondrial membrane suspension [Fe2+]. value increased from 10 to 40 mu kM with increasing membrane concentrations from 1 to 4 mg/ml. Apparently, [Fe2+]. value is the main parameter determining the kinetics of Fe(2+)-induced lipid peroxidation in native membrane preparations; the [Fe2+]. value in different membrane systems, in turn, is dependent on the ability of the membrane to bind Fe2+ ions.