[The ultrastructure and function of lymphocytes in children with first detected untreated type 1 diabetes mellitus].

Flow cytometry (FACS-analysis), light and electron microscopy, and cytotoxic test were used to study the leukocytic composition, content, ultrastructure, and function of different lymphocytic populations (CD3+, CD4+, CD8+, CD20+, and CD56+ cells) in the blood of 90 children aged 8 to 15 years who had untreated new-onset type 1 diabetes mellitus. A control group consisted of 45 apparently healthy normoglycemic children. The sick children were observed to have a slight, but statistically significant reduction in the relative and absolute blood content of CD3+, CD4+, and CD56+ cells, CD4/CD8 index and particularly large granule-containing lymphocytes (the morpho-logical homologue of natural Idller (NK) cells). Ultrastructurally, CD20+4 cells (lymphocytes containing Gall's bodies) showed the signs of a high functional activity while NK cells (large granule-containing lymphocytes) exhibited a low one, which was confirmed by the decreased cytotoxic activity of these cells determined in vitro. Three-month insulin therapy leading to the restoration of the blood content of glucose and glycosylated hemoglobin fails to normalize the detected changes in the parameters of T- and NK-cell immunity.

[Application of immunomodulators for correction of changes in the immune system in conditions of experimental hypothyroidism]. / Vykorystannia imunomoduliatoriv dlia korektsiï zmin v imunnii systemi v umovakh eksperymental'noho hipotyreozu.

Changes of the immune system state were investigated in mice after performance of thyroidectomy under the influence of hormonal therapy using thyroxin and immunotropic preparations thymogen and polyoxydonium. In hypothyrosis there was observed the trustworthy lowering of the thymus endocrine function, interleukine-2 activity, cytotoxic activity in the physiological cell-killers, the leukocytes proliferating ability and increase of the blood content of circulating immune complexes as well. The restorational therapy conduction using thyroxin, injections of thymogen or polyoxydonium removed the above mentioned changes only in part. The immunomodulators application, under condition of the hypothyrosis compensation occurrence, have had promoted the essential raising of their therapeutic action and secured the immune state restoration after thyroidectomy performance.

[Immune state of patients of various age with the thyroid gland tumor]. / Immunnyi status bol'nykh razlichnogo vozrasta s novoobrazovaniem shchitovidnoi zhelezy.

The comparative analysis of immune state of the various age patients with benign and malignant thyroid gland (TG) tumors is presented. In the TG cancer the immunogenesis insufficiency is more pronounced. The data obtained trusts the perspectiveness of the immunocorrecting therapy application in patients with the TG cancer.