RESUMO
MicroRNA-21 (miR-21) expression was quantified by real-time qRT-PCR in peripheral blood and saliva samples obtained from patients diagnosed with colorectal cancer (CRC) of varying degrees of malignancy and healthy volunteers. All patients had adenocarcinoma located in the distal colon at different stages. Significant differences were detected between the control group and the total experimental group of CRC patients (plasma, P = 0.0001; saliva, P = 5e-12). MiR-21 expression was also significantly different in certain subgroups of patients with CRC disease stages II-IV as compared to the control group. No correlation of miR-21 expression was found with regard to gender and age of patents. Also, there were no significant individual correlations and linear regression of miR-21 expression in the plasma and saliva. The estimated diagnostic sensitivity and specificity of miR-21 expression were respectively 65 and 85% in the plasma, and 97 and 91% in the saliva. Our data suggest that miR-21 in both the saliva and plasma could be a proper biomarker for CRC screening, although the saliva miR-21 expression test looks preferable due to its higher sensitivity, specificity, and technical simplicity.
Assuntos
Neoplasias Colorretais/genética , MicroRNAs/genética , Saliva/química , Idoso , Idoso de 80 Anos ou mais , Biomarcadores Tumorais/análise , Biomarcadores Tumorais/sangue , Biomarcadores Tumorais/genética , Estudos de Casos e Controles , Feminino , Humanos , Masculino , MicroRNAs/análise , MicroRNAs/sangue , Pessoa de Meia-Idade , Estadiamento de Neoplasias , Sensibilidade e EspecificidadeRESUMO
The increasing popularity of the concept of liquid biopsy, which involves the definition of circulating tumor cells and circulating nucleic acids in the blood can significantly improve long-term results of treatment for gastric cancer due to the high sensitivity of techniques. It needs further study in relation to gastric cancer, the search for universal markers and methods.
Assuntos
Biomarcadores Tumorais/sangue , DNA Tumoral Circulante/sangue , Biópsia Líquida , Neoplasias Gástricas/sangue , Humanos , MicroRNAs/sangue , Células Neoplásicas Circulantes/metabolismo , Células Neoplásicas Circulantes/patologia , Neoplasias Gástricas/patologiaRESUMO
The authors developed and described an algorithm and technical aspects of lymphadenectomy performance in radical surgery based on structural variations of visceral vessels in 91 patients with stomach cancer. Preoperative helical computer tomography in regimen of angiography was carried out on 91 patients. Variant anatomy of visceral vessels was detected in 31 patients and that required change of surgical technique in order to minimize intraoperative risks of great vessel damage and allowed perforrming lymphadenectomy successfully.
Assuntos
Algoritmos , Angiografia/métodos , Artéria Celíaca/diagnóstico por imagem , Gastrectomia/métodos , Excisão de Linfonodo/métodos , Neoplasias Gástricas/secundário , Neoplasias Gástricas/cirurgia , Tomografia Computadorizada Espiral , Humanos , Metástase Linfática , Estudos Retrospectivos , Neoplasias Gástricas/irrigação sanguíneaRESUMO
Standard methods of diagnostics haven't got a proper sensitivity and specificity concerning assessment of regional nodal involvement in case of rectal cancer. Therefore it is necessary to look for new method of diagnostics, detect risk factors and unfavorable prognosis in relation to lateral lymph node metastasis. At the same time, there should be a differentiated approach to the choice of therapeutic management in rectal cancer. Investigation of variability of blood supply of the rectum could be an additional method of diagnostics in rectal cancer.
Assuntos
Linfonodos/patologia , Neoplasias Retais/secundário , Medição de Risco/métodos , Humanos , Metástase Linfática/diagnóstico , Pelve , Prognóstico , Neoplasias Retais/diagnóstico , Fatores de RiscoAssuntos
Neoplasias do Colo/cirurgia , Procedimentos Cirúrgicos do Sistema Digestório/métodos , Neoplasias Primárias Múltiplas/cirurgia , Neoplasias Gástricas/cirurgia , Malformações Vasculares/complicações , Vísceras/irrigação sanguínea , Neoplasias do Colo/diagnóstico , Diagnóstico Diferencial , Endoscopia do Sistema Digestório , Humanos , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada Multidetectores , Neoplasias Primárias Múltiplas/diagnóstico , Neoplasias Gástricas/diagnóstico , Malformações Vasculares/diagnóstico , Gravação em VídeoAssuntos
Adenocarcinoma/patologia , Colectomia/métodos , Descompressão Cirúrgica/métodos , Colo Sigmoide/diagnóstico por imagem , Colo Sigmoide/patologia , Colo Sigmoide/cirurgia , Humanos , Obstrução Intestinal/etiologia , Masculino , Pessoa de Meia-Idade , Tomografia Computadorizada Multidetectores , Invasividade Neoplásica , Estadiamento de Neoplasias , Neoplasias do Colo Sigmoide/complicações , Neoplasias do Colo Sigmoide/patologia , Resultado do TratamentoRESUMO
In order to improve radicalism of emergency surgical interventions an appropriate method of total mesocolonectomy and D3-lymph node dissection in the medial-lateral direction and the principle of "no-touch technique" were used in 14 patients. This technique is safe, effective, does not prolong the duration of operation and postoperative period, is not followed by increased number of complications and lethality.
