Evaluation of a 4% gelatin solution in the treatment of canine haemorrhagic shock.

The effects of volume replacement with 4% gelatin containing 1% glycine at pH 5.8 and 6.4 on the changes in mean arterial blood pressure (MABP) and blood levels of glucose, lactate and urea following acute haemorrhage in dogs were studied. Comparison was made with dogs given homologous blood. Dogs bled 30% of blood volume, maintained in shock for 30 min, and infused with gelatin solutions, showed values of MABP, blood glucose, lactate and urea nitrogen insignificantly different from dogs similarly bled and infused with homologous blood. The responses obtained in dogs infused with gelatin at pH 5.8 were better than at pH 6.4.

Effects of metaraminol, insulin and trasylol on the responses to haemorrhage in dogs.

The effect of addition of metaraminol, insulin and trasylol to fluid replacement with glucose-saline, on the responses to haemorrhage (30% of blood volume) in dogs, was investigated. Addition of metaraminol significantly increased the blood sugar and lactate responses to haemorrhage; the lactate-pyruvate (L/P) ratio, plasma phosphate and free fatty acid (FFA) levels were also significantly increased. A delayed hypotension was observed. Responses to haemorrhage during infusion of glucos-saline + insulin showed reduced blood sugar, blood lactate, pyruvate, and L/P ratios, plasma inorganic phosphate and FFA were diminished while, mean arterial blood pressure (MABP) changes were significantly higher than the glucose-saline group. Infusion of trasylol in glucose-saline did not significantly affect the blood sugar, lactate, and pyruvate resonses and the L/P ratio. Plasma phosphate response was also like the control group. The responses of plasma FFA and of MABP in this group were significantly higher than glucose-saline group. The mechanisms of these effects and the significance of thse results are discussed.