RESUMO
The effect of antisense oligonucleotides specific to mRNA of the proapoptotic gene harakiri (Hrk) on the development of mouse SAMP1 (senescence-accelerated mouse prone) and (C57BL/6J x DBA/2J)F1 preimplantation embryos cultured in vitro was investigated. The SAMP1 mice are characterized by genetically determined decrease of fertility along with the highly frequent perturbations of embryonic development. Reproduction indices of the (C57BL/6J x DBA/2J) hybrids lie within the normal range. Because of this, preimplantation abnormalities in this line were induced by the action of proapoptotic agent bleomycine. It was demonstrated that antisense inhibition of the Hrk expression had no effect on the frequency of genetically determined abnormalities of early embryonic development in SAMP1 mice. In case of induced abnormalities, addition of oligonucleotides specific to mRNA of proapoptotic Hrk gene influenced the number of abnormalities, and at the same time, improved the quality of survived embryos via increasing the blastocyst hatching.
Assuntos
Proteínas Reguladoras de Apoptose/antagonistas & inibidores , Blastocisto/metabolismo , Embrião de Mamíferos/embriologia , Desenvolvimento Embrionário/efeitos dos fármacos , Neuropeptídeos/antagonistas & inibidores , Oligodesoxirribonucleotídeos Antissenso/farmacologia , RNA Mensageiro/antagonistas & inibidores , Animais , Proteínas Reguladoras de Apoptose/biossíntese , Proteínas Reguladoras de Apoptose/genética , Embrião de Mamíferos/anormalidades , Desenvolvimento Embrionário/genética , Feminino , Masculino , Camundongos , Neuropeptídeos/biossíntese , Neuropeptídeos/genética , Oligodesoxirribonucleotídeos Antissenso/genética , RNA Mensageiro/biossíntese , RNA Mensageiro/genéticaRESUMO
Colloid-osmotic blood pressure (COP) was measured for the first time in 56 neurosurgical patients by means of an oncometer made by the "Knauer" Firm. The examination was conducted in dynamics: prior to operation, on the 1st, 5th and 10th postoperative days. The control group was formed of 25 donors. The study showed that before the operation the COP in neurosurgical patients depends on the character of the disease. In neuro-oncological patients and in patients with diseases of the cerebral vessels of COP was increased prior to the operation. It decreased immediately after the operation, remained low on the 5th postoperative day and gradually returned to normal level on the 10th day of treatment. In the decompensation phase, the COP remained drastically low till the patient's death. The reduction of COP was mainly caused by a fall in the total protein concentration. No correlation was found between COP and osmolality, its components, the value of pH, hematocrit and albumin concentration. The value of COP is a highly informative test for appraising protein and water metabolism. It provides for the purposeful application of substitution therapy and parenteral and enteral feeding in neurosurgical patients.
