Alpine grazing management, breed and diet effects on coagulation properties, composition, and microbiota of dairy cow milk by commercial mountain based herds.

Cow milk microbiota has received increased attention in recent years, not only because of its importance for human health but also because of its effect on the quality and technological properties of milk. Several studies, therefore, have investigated the effect of various production factors on the microbial composition of milk. However, most of the previous studies considered a limited number of animals from experimental or single farm, which could have biased the results. Therefore, this study aimed to understand the effect of different alpine production systems on the compositional and microbiological quality of milk, considering commercial herds with different feeding intensities and cattle breeds. The results obtained in this work indicated that the month/season of sampling (July for summer or February for winter) more than farm, breed and cow diet exerted significant effects on cow milk parameters and microbiota. In particular, significant differences were observed for urea content in milk between sampling seasons. Differences in milk fat were mainly related to breed specific effects. From a microbiological point of view, statistically significant differences were found in presumptive lactic acid bacteria counts. Based on a culture-independent method, milk obtained in February harbored the highest number of Firmicutes (e.g., Lactobacillus) and the lowest number of Actinobacteria (e.g., Corynebacterium). Moreover, bacterial richness and diversity were higher in July/summer during alpine pasture season indicating a significant effect of pasture feed on the growth of bacterial communities. The results of this study highlighted the effect of month/season mainly related to differences in feeding management (e.g., access to pasture during vegetation period, concentrates supplementation) on composition and microbiota in milk.

Assessing the impact of biosecurity practices and animal welfare in small-scale mountain dairy farming.

This study estimates the association between the level of biosecurity, animal welfare, milk quality, and economic performance on 2291 mountain dairy farms which largely differs in management and structure from large dairy operations in the lowlands mainly due to climatic and topographic constraints in mountain areas. The dairy industry's increasing emphasis on biosecurity is crucial for ensuring animal health, productivity, and disease mitigation. Therefore, in the present study the biosecurity and animal welfare status of mountain dairy farms were assessed considering the official welfare protocol for dairy cows of the Italian ClassyFarm system. Our findings reveal a suboptimal adoption of biosecurity measures, attributed to structural limitations in mountain farms and farmers' awareness gaps. Despite these challenges, the economic significance of biosecurity adoption is evident, emphasizing potential benefits for farm viability and animal health. Conversely, the study indicates a moderate to good welfare status in assessed farms, reflecting farmers' awareness of the importance of high welfare standards for dairy cows. Improved welfare positively correlates with milk sales and productivity, highlighting the economic advantages of prioritizing animal well-being. Challenges persist, particularly regarding animal housing deficiencies and related consumer concerns about animal welfare in dairy production. Therefore, targeted interventions and educational initiatives are crucial to empower farmers and promote best practices in biosecurity and animal welfare management. However, addressing resultant production cost increases necessitates society's willingness to pay more for animal-based food, emphasizing the importance of aligning economic incentives with sustainable farming practices.

Exploring the heights: Impact of altitude on dairy milk composition.

The aim of the present study was to assess the impact of altitude on the quality of milk from dairy cows housed in small-scale farms in alpine areas. Therefore, a dataset comprising 5,680 bulk milk samples from 32 farms located at different geographical altitudes was considered. Milk traits such as fat, protein, lactose, free fatty acids, casein, milk urea nitrogen content, pH value, and somatic cell score were examined in the laboratories of the South Tyrolean Dairy Association using mid-infrared spectroscopy. The data were analyzed using a statistical model that considered altitude category, grazing practice, housing system, and season of milk analysis as fixed effects. The findings revealed a positive association between altitude and milk fat, free fatty acids, and somatic cell count. Conversely, lactose content, milk urea nitrogen, and pH value in milk from cows kept on farms at higher altitude (>1,200 m above sea level) showed a negative relationship with altitude. Farms located at altitudes above 1,200 m exhibited higher fat, protein, urea, and somatic cell content than farms located at lower levels. The results provide new insights into a production effect that has so far received little attention and should be considered in farm management (e.g., feeding management, breed decision) to ensure animal health and the associated animal welfare as well as the productivity of dairy cows reared in traditional small-scale mountain dairy farms.

Effect of slaughter age on environmental efficiency on beef cattle in marginal area including soil carbon sequestration: A case of study in Italian Alpine area.

The production of beef carries significant environmental repercussions on a worldwide level. Considering that the production of beef in Alpine mountainous regions, such as South Tyrol (Italy), constitutes a modest yet progressively growing segment within the local agricultural sector focus must be put on minimizing the environmental impact of producing one kilogram of meat, while also accounting for the carbon sequestered by Alpine pastures in such marginal areas. To this end 20 beef farms distributed in the South Tyrolean region (Italy) were divided based on the age at slaughter of the beef cattle: 10 farms with a slaughter age of 12 months (SA12) and 10 farms with a slaughter age of 24 months (SA24). Live cycle assessment (LCA) approach was used, and the impact was estimated using two functional units (FU): 1 kg of live weight (LW) and 1 kg of carcass weight (CW). Global warming potential (GWP100, kg CO2-eq), acidification potential (AP, g SO2-eq), and eutrophication potential (EP, g PO4-eq) were investigated. Furthermore, within the account, the carbon sequestered by pastures and permanent grassland has been included for estimated the overall carbon footprint. In terms of GWP100, the SA12 system proved to be significantly lower for both two functional units under studies, with reductions of 8.5 % and 7.4 % in terms of LW and CW, respectively, compared to the SA24 system, specifically, the SA12 system showed an environmental impact in terms of GWP100 of 19.5 ± 1.1 kg CO2-eq/kg LW, which was significantly lower than the SA24 system that exhibited a value of 22.9 ± 1.1 kg CO2-eq/kg LW (P < 0.05). When accounting for the carbon sequestered within the system, the observed values in terms of GWP100 are significantly lower for SA12 compared to SA24, 17.6 ± 1.5 vs. 20.9 ± 1.5 kg CO2-eq/Kg LW (P < 0.05), and 29.2 ± 2.5 vs. 38.7 ± 2.5 kg CO2-eq/Kg CW (P < 0.01). These differences are due to less purchase of concentrated feed and greater use of natural resources such as pastures and permanent grasslands. The research indicated that the production of beef in the Alpine region of South Tyrol predominantly occurs within extensive parameters, leading to a satisfactory environmental profile, also including the C sequestration.

Associated risk factors for skin alterations in dairy cattle kept on small scale mountain farms.

The objective of this study was to determine the risk factors for skin alterations at herd and cow level on dairy farms with different housing systems in South Tyrol (Northern Italy). A cross-sectional study was conducted on 204 farms (111 free stalls and 93 tie stalls) from March to October 2019 assessing the level of animal welfare using resource-based and animal-based indicators. A total number of 1,891 dairy cows were evaluated, of which 43.5% were reared in tie stalls and 56.5% in free stalls. A logistic regression model identified the herd and cow level risks factors for neck and leg skin alterations in the two different systems. There was a higher prevalence for skin lesions on the neck (Odd Ratio (OR) = 2.36) and hock (OR = 2.82) for tie stalls. Irrespective of the housing system the soft-based stall mattresses had a lower prevalence for knee and hock lesions of 0.48 and 0.54, respectively, compared to wood base stalls for both knee (OR = 2.19) and hock (OR = 2.47) consecutively. The prevalence of skin alterations on the knee (OR = 0.42) and hock (OR = 0.33) decreased by the presence of sawdust as bedding material. Similarly, straw (OR = 0.61) and lime-straw-water bedding (OR = 0.59) reduced the prevalence for skin alterations on the hock. Access to pasture reduced the prevalence of skin alterations on the neck (OR = 0.34), the knee (OR = 0.77), and on the hock (OR = 0.46) regardless of the housing system. In conclusion, the assessment of risk factors of different skin alterations in different housing systems can contribute to the improvement of overall animal welfare in traditional small scale mountain dairy systems.

Quantifying methane emissions under field conditions under 2 different dairy production scenarios: Low-input versus high-input milk production.

Livestock production systems with ruminants play a relevant role in the emission of the greenhouse gas CH4, which is known to significantly contribute to global warming. Consequently, it is a major societal concern to develop strategies in mitigating such emissions. In addition to breeding toward low-emitting cows, management strategies could also help in reducing greenhouse gas emissions from dairy farms. However, information is required for appropriate decision making. To the best of our knowledge, this is the first study that considers different, already available equations to estimate CH4 emissions of small-scale dairy farms in the mountain region, which largely differ from large dairy farms in the lowlands concerning management and production. For this study, 2 different production systems, both typical for small-scale dairy farming in mountain regions, were simultaneously run over 3 yr at an experimental farm as follows: (1) a high-input production system, characterized by intensive feeding with high amounts of external concentrates and maize silage, year-round housing, and high yielding Simmental cattle breed, and (2) a low-input production system, characterized by prevailing hay and pasture feeding and silage ban, thus covering most of the energy requirements by forage harvested on-farm and the use of the local Tyrolean Grey cattle breed. Results reveal that feeding management has a significant effect on the amount of CH4 emissions. The low-input production system produced less CH4 per cow and per day compared with the high-input production system. However, if calculated per kilogram of milk, the high-input scenario produced proportionally less CH4 than the low-input one. Findings of this study highlight the potential to assess in a fast and cost-effective way the CH4 emission in different dairy production systems. This information contributes to the debate about the future of sustainable milk production in mountain regions, where the production of feed resources is climatically constrained, and could be useful for breeding purposes toward lower CH4-emissions.

500 GHz mode-hop-free idler tuning range with a frequency-stabilized singly resonant optical parametric oscillator.

A 1064 nm pumped continuous-wave, mid-IR (3-4 µm), signal-wave resonant optical parametric oscillator is frequency stabilized at the kilohertz jitter level to the transmission peak of an external high-finesse Fabry-Perot cavity. Owing to the high stability of the resonator length against acoustical perturbation, fine pump tuning of the idler wave around 3.3 µm results in an unprecedented mode-hop-free continuous scan over 500 GHz (17 cm⁻¹).

Observation of strong cascaded Kerr-lens dynamics in an optimally-coupled cw intracavity frequency-doubled Nd:YLF ring laser.

Self-starting self-pulsing dynamics at the cavity free-spectral-range frequency were observed in intracavity second-harmonic generation of a diode end-pumped Nd:YLF ring laser containing a periodically-poled KTiOPO(4) (ppKTP) nonlinear crystal. Although the unidirectional laser was designed for continuous-wave (cw) single-frequency operation, with a resonator set at the middle of its stability range, partial Kerr-lens mode-locking (KLM) arose spontaneously once the ppKTP was inserted. This ultrafast dynamics along with a strong spectral gain broadening, not observed with any birefringent nonlinear doubler, is associated to the finite bandwidth of the quasi-phase-matched crystal with respect to the laser gain bandwidth, leading to giant cascaded Kerr-lensing effects when the ppKTP temperature is detuned from perfect quasi-phase-matching either in the self-focusing or defocusing sides. While under partial KLM operation the laser delivered only approximately 0.14W of broadband red output power, single-frequency operation could be only achieved by using an intracavity etalon with a suitable partial reflectivity (R>or=25%), leading to an optimally (approximately100% efficiency) out-coupled 1.4 W red power at 660.5 nm, as much as the fundamental 1321 nm power that could be extracted from the unidirectional laser using an optimal T = 2% output coupler.