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1.
J Thromb Haemost ; 14(6): 1268-84, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-26991240

RESUMO

UNLABELLED: Essentials Information about the formation of the demarcation membrane system (DMS) is still lacking. We investigated the role of the cytoskeleton in DMS structuration in megakaryocytes. Cdc42/Pak-dependent F-actin remodeling regulates DMS organization for proper megakaryopoiesis. These data highlight the mandatory role of F-actin in platelet biogenesis. SUMMARY: Background Blood platelet biogenesis results from the maturation of megakaryocytes (MKs), which involves the development of a complex demarcation membrane system (DMS). Therefore, MK differentiation is an attractive model for studying membrane remodeling. Objectives We sought to investigate the mechanism of DMS structuration in relationship to the cytoskeleton. Results Using three-dimensional (3D) confocal imaging, we have identified consecutive stages of DMS organization that rely on F-actin dynamics to polarize membranes and nuclei territories. Interestingly, microtubules are not involved in this process. We found that the mechanism underlying F-actin-dependent DMS formation required the activation of the guanosine triphosphate hydrolase Cdc42 and its p21-activated kinase effectors (Pak1/2/3). Förster resonance energy transfer demonstrated that active Cdc42 was associated with endomembrane dynamics throughout terminal maturation. Inhibition of Cdc42 or Pak1/2/3 severely destructured the DMS and blocked proplatelet formation. Even though this process does not require containment within the hematopoietic niche, because DMS structuration was observed upon thrombopoietin-treatment in suspension, integrin outside-in signaling was required for Pak activation and probably resulted from secretion of extracellular matrix by MKs. Conclusions These data indicate a functional link, mandatory for MK differentiation, between actin dynamics, regulated by Cdc42/Pak1/2/3, and DMS maturation.


Assuntos
Actinas/metabolismo , Megacariócitos/metabolismo , Proteína cdc42 de Ligação ao GTP/química , Proteína cdc42 de Ligação ao GTP/metabolismo , Animais , Plaquetas/metabolismo , Diferenciação Celular , Linhagem Celular Tumoral , Membrana Celular/metabolismo , Citoesqueleto/metabolismo , Transferência Ressonante de Energia de Fluorescência , Humanos , Imageamento Tridimensional , Lentivirus , Camundongos , Camundongos Endogâmicos C57BL , Microscopia Confocal , Microscopia Eletrônica de Transmissão , Transdução de Sinais , Trombopoese , Quinases Ativadas por p21/metabolismo
2.
Neurochirurgie ; 43(2): 76-84, 1997.
Artigo em Francês | MEDLINE | ID: mdl-9296049

RESUMO

Radiological follow-up of ethmoidal neoplasms is not clearly established in terms of imaging modalities (CT, MRI) or periodicity. This paper, based on 4 typical cases, tries to describe the main imaging features of this radiological follow-up. Initial CT 3 to 6 months after surgery is essential, being the reference examination. It can show postoperative bone changes. During follow-up, a CT scanner every 6 months seems sufficient. For the image interpretation, a comparison with the two previous CT is mandatory. Any bone destruction must be considered as suspicious, even if very small. MRI should be performed in case of sphenoidal opacity, in order to differentiate between tumor recurrence and retentional fluid. MRI is also necessary and useful when the tumor presents an intradural or intracranial extension.


Assuntos
Seio Etmoidal , Neoplasias dos Seios Paranasais/diagnóstico por imagem , Adenocarcinoma/diagnóstico por imagem , Adenocarcinoma/cirurgia , Carcinoma/diagnóstico por imagem , Carcinoma/cirurgia , Humanos , Imageamento por Ressonância Magnética , Masculino , Osteólise/diagnóstico , Osteólise/diagnóstico por imagem , Neoplasias dos Seios Paranasais/cirurgia , Período Pós-Operatório , Fatores de Tempo , Tomografia Computadorizada por Raios X
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