Circulating miR-378 in plasma: a reliable, haemolysis-independent biomarker for colorectal cancer.

BACKGROUND: Plasma circulating tumour-specific microRNAs (miRNAs) are promising biomarkers of tumour presence and recurrence, especially for diseases whose best chance of successful treatment requires early diagnosis and timely surgery of an already malignant but not yet invasive tumour, such as colorectal cancer (CRC). METHODS: Expression levels of miRNAs previously found to be differently expressed in tumour vs normal colon tissues were investigated by quantitative real-time PCR in plasma from CRC patients and from healthy donors and confirmed in independent case control series. The validated miRNAs were also measured after surgery. Analyses were repeated on the subsets of haemolysis-free samples. RESULTS: We identified four miRNAs differently expressed between the compared groups, two (miR-21 and miR-378) of which were validated. miR-378 expression decreased in non-relapsed patients 4-6 months after surgery and miR-378 ability to discriminate CRC patients from healthy individuals was not influenced by haemolysis levels of plasma samples. CONCLUSION: The miRNA analysis on plasma samples represents a useful non-invasive tool to assess CRC presence as well as tumour-free status at follow-up. Plasma levels of miR-378 could be used to discriminate CRC patients from healthy individuals, irrespective of the level of haemoglobin of plasma samples.

The development of biomedical engineering.

This paper discussed how the bioengineering and medical engineering started in Argentina.

Canine piroplasmosis in Italy: epidemiological aspects in vertebrate and invertebrate hosts.

In Italy, canine piroplasmosis is believed to be widespread, but few data are available on its presence in most areas. In 2005 and 2006, vertebrate and invertebrate hosts were investigated in Central and Northern Regions of the Country. Microscopy on blood smears, molecular tools and serological tests were applied to 420 blood samples collected from dogs, in order to evaluate the presence of these protozoa and to identify possible risk factors. Moreover, ticks were analyzed by molecular techniques. Microscopy identified as positive 2.8% of the animals, all from Central Italy, and PCR detected 'piroplasm' DNA in 6.0%. Serology evidenced a mean prevalence of 34.0% with a decreasing trend from Central to Northern areas. The 507 collected ticks were identified as belonging to 8 species, mostly represented by Rhipicephalus sanguineus (n=376) and Ixodes ricinus (n=58). Molecular analyses evidenced the presence of babesial parasites (Babesia canis canis, B. canis vogeli, B. microti-like) in 25 (4.9%) of them; in Rh. sanguineus there was also demonstration of the vertical transmission of B. canis canis. Statistical analysis identified 'kennel' as risk factor for Babesia infection. Our findings evidenced that different species of piroplasms potentially infectious for dogs are circulating in Italy, and that epidemiological aspects of these infections are more complex than expected. Vector importance of both Rh. sanguineus and I. ricinus is hypothesized, but further investigation is needed.

A neural network model of memory under stress.

A model that attempts to simulate animal memory under stress is presented. For this purpose a model of selectable multiple associative memories is given. We consider two underlying types of memories: stressed and unstressed, implemented on the same neural network. In our model, learning into one or the other type of memory is done according to the stress of the individual at the time of learning. Memory retrieval is obtained according to a continuous function of the stress of the individual at the time of retrieval, who for low stress retrieves unstressed associations and for high stress retrieves stressed associations. Several biological results supporting this model are presented. A mathematical proof on the behaviour of the basins of attraction of the network as a function of stress is presented. Also a generalization to selectable multiple coexisting memories is given, and engineering and other applications of the model are suggested.

Pitch perception in patients with a multi-channel cochlear implant using various pulses width.

Cochlear implants have been designed to partially restore hearing to those people who are totally deaf. Multi-channel cochlear implants offer the opportunity to evoke acoustic perceptions like loudness and pitch, elicited by a controllable pattern of electric stimulation by means of electrodes placed in different places along the cochlear length. In this study, two psychophysical experiments were conducted with 4 patients, 1 prelingually and 3 postlingually-deafened, implanted with the multi-channel cochlear prosthesis Nucleus 22. Experiments were carried out to study the effect of varying the width of the electric biphasic pulsatile stimuli on the discriminative abilities of the pitch perception. The tests involved place pitch ranking and pulse rate discrimination. Place pitch ranking was studied by determining the just noticeable difference in pitch pairs (jnd-pp), defined as the pair of nearest electrodes which elicit different pitch perception. Pulse rate discrimination was studied by determining the just noticeable difference in pulse rate (jnd-pr) defined as the minimal difference in stimulus repetition rate over a given electrode, which elicits different pitch perceptions. Both experiments were conducted using pulses of 400, 200, 100 and 50 microseconds/phase. The results indicated that in spite of the differences in pathologies and personal histories, both jnd-pp and jnd-pr decrease by diminishing the pulse width. Speech perceptual data, measured for various pulse widths, validates the usefulness of decreased pulse width which yields favorable results in the psychophysical tests.

Extracochlear electrical stimulation.

Twelve patients with sensorineural hearing loss were stimulated by a transtympanic electrode contacting the cochlear promontory. Nine of them were tested to study hearing perception associated with various features of electrical signals, using an auditory electrical stimulator. Biphasic pulse bursts and sine-wave bursts were used as stimuli. Different electrical characteristics such as amplitude, width and rate for pulses and amplitude and frequency for sine waves were used to investigate hearing perception. The patients' perception threshold, comfortable level, uncomfortable level, dynamic range, just-noticeable difference in frequency or pitch discrimination, just-noticeable difference in intensity or loudness discrimination, loudness perception associated with the electrical signal energy, and sounds associated with electrical stimulation was determined. Pertinent results assisted in the development of a speech electrical stimulator that was used to test three patients for vowel, word, and consonant identification and recognition of patterns of intonation. These results in turn are being used to design a prototype of a single-channel extracochlear prosthesis.