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5.
Curr Protoc Mol Biol ; Chapter 21: Unit 21.1, 2005 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18265356

RESUMO

This unit describes methodology for using micrococcal nuclease to investigate the presence of nucleosomes at a particular location in chromatin and to map the positions of nucleosomes at various levels of resolution. The approaches are readily adaptable to other probes of chromatin structure that cause DNA cleavage. Results obtained from such chromatin studies provide a structural view of the molecular environment of gene in their native context in cells.


Assuntos
Cromatina/química , Nuclease do Micrococo/metabolismo , Núcleo Celular/química , DNA/análise , Clivagem do DNA , Nucleossomos/química
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