[Experience in immunomodulators application for comprehensive dental caries prevention in children and teenagers].

Prophylaxis and treatment procedures in children with different dental status was done during 9 years. Children and teenagers of 6-12 and 15-years were given teeth treatment and professional hygiene. One group of patients was additionally treated by immunomodulators imudon. It was demonstrated that improving of oral cavity hygiene and local immunity factor balance was achieved. Positive result was better in the group with imudon.

[Role of nuclear factor-kappaB in providing interactions of human neutrophils and epithelial cells from oral cavity with Candida albicans].

Contribution of intracellular signal pathways associated with nuclear factor kappaB (NF-kappaB) in realization of interactions of human neutrophils and epithelial cells from oral cavity with Candida albicans was studied. Supression of NF-kappaB in epitheliocytes resulted in decreased adsorption of C. albicans to buccal cells (buccal mucosa). Inhibition of NF-kappaB led to decrease in receptor-dependent oxidative activity of neutrophils in systems with native and IgG-opsonized C. albicans as well as to increase of phagocytic activity during contact with C3b-/iC3b-opsonized C. albicans. It has been concluded that NF-kappaB participates in regulation of specific interactions of neurophils and epitheliocytes with C. albicans.

[Antiadhesive effect of the secretion of the oral cavity in the system "Candida albicans--buccal epitheliocytes"].

The influence of the oral cavity secretion on adhesive reactions in the system "C. albicans--buccal epitheliocytes" was studied. The treatment of C. albicans with natural saliva led to decrease of adhesive activity. The treatment of C. albicans with antibody absorbed saliva at different temperature conditions led to different changes of adhesion. This effect was determined by the action of temperature-dependent and temperature-independent factors, supposedly of enzymatic nature.

[Influence of staphylococcal metabolites on adhesive reactions in the system "Candida albicans-buccal epitheliocytes"]. / Vliianie metabolitov stafilokkov na adgezivnye reaktsii v sisteme "Candida albicans-bukkal'nye epiteliotsity".

The influence of S. aureus and S. epidermitidis metabolites on the adhesive reactions in the system "C. albicans-buccal epitheliocytes" was studied. The study revealed that the treatment of C. albicans with S. aureus supernatants inhibited the adhesion of C. albicans to epitheliocytes, the degree of the inhibiting action of S. aureus supernatants in the system depending on their strain specificity. S. epidermitidis supernatants produced no adhesive effect. The irreversible decrease of the adhesive activity of C. albicans under the action of bacterial metabolites was, seemingly, the consequence of transformation of the receptor apparatus of C. albicans. At the same time S. aureus supernatants produced no essential influence on the adhesive potential and viability of buccal epitheliocytes.

[Reactivity of Candida albicans in the system of the alternative way of complement activation]. / Reaktivnost' Candida albicans v sisteme al'ternativnogo puti aktivatsii komplementa.

The neutrophil-stimulating activity of C. albicans before and after opsonization in the system of the alternative way of complement activation (AWCA) was studied. The study revealed that, in comparison with zymosan, C. albicans exhibited a considerably lower index of AWCA-dependent opsonic effect in reaction with neutrophils. This did not correlate with the capacity of substrates to activate the alternative cascade and with the intensity of phagocytosis. The suggestion on the involvement of C. albicans thermolabile cell-wall components into the negative regulation AWCA-dependent opsonic effect was substantiated.

[A method for measuring the strength of adhesion of Candida albicans on epithelial cells]. / Sposob otsenki prochnosti adgezii Candida albicans na épiteliotsitakh.

A method on estimation of Candida albicans adhesion to epitheliocytes is proposed. The principle consists in the fact that while centrifuging epitheliocytes (1000 cycles/min, 10 min) with adhered Candida albicans through phycoll-verografin (1.077 g/cm3 density), Candida albicans cells having poor adhesion are separated from epitheliocytes trying to rise to the surface of phycoll-verografin. Candida albicans strongly adhered to epitheliocytes settle down to the bottom of test tube. In experiments with buccal epitheliocytes of healthy donors the number of strongly adhered Candida albicans ranged from 13% to 50% and they amounted to 37.0 +/- 7.8% on an average.

[Phenomenon of selective decrease of colonization (adherence) resistance in the system "Candida albicans-buccal epithelial cells"]. / Fenomen izbiratel'nogo oslableniia kolonizatsionnoi (adgezivnoi) rezistentnosti v sisteme "Candida albicans-bukkal'nye épiteliotsity".

In the period of the exacerbation of bronchial asthma an increased adhesiveness (in vitro) of buccal epithelial cells to C. albicans was noted in most children under study (94.7%). This phenomenon was not observed in children with the exacerbation of gastroduodenitis. The characteristics of natural bacterial colonization of buccal epithelium were equally decreased in both groups of patients. These results are regarded as the consequence of the reactive involvement of the epithelium of mucoid tract in the processes destabilizing homeostasis.

[IgG- and C3-dependent adhesion of neutrophils in systems with allogeneic and xenogeneic ligands]. / IgG- i C3-zavisimaia adgeziia neitrofilov v sistemakh s allogennymi i ksenogennymi ligandami.

Interactions of human and rat neutrophils with allogenic and xenogeneic ligands were investigated. Human blood neutrophils reacted equally with human and rat IgG; IgG-dependent adhesion of rat neutrophils (from aseptic peritoneal exudate or blood) was observed only in allogenic system. C3-dependent reactions of neutrophils were observed both in allogenic and xenogeneic systems. The advantage of allogenic combinations in this case was reliable only for rat neutrophils. The relation of obtained results to structural and functional heterogeneity of receptors and ligands is discussed.