[Evaluation of the the effectiveness of a newly developed tissue-inactivated vaccine against rabies using the NIH test]. / Hodnocení úcinnosti vyvíjené vakcíny proti vztekline tkánové inaktivované NIH testem.

NIH test was used to verify 12 operation batches of the newly developed inactivated tissue vaccine against rabies. The number of international units per 1 cm3 of vaccine was determined by the volumetric method. The number of international units (I.U.) per 1 cm3 of the newly developed vaccines ranged from 1.08 I.U. to 2.59 I.U. per cm3. The average value of all the 12 batches tested is 1.52 I.U. per cm3. The average value of international units in the vaccination dose for dogs is 4.57. All the tested operation batches of the newly developed vaccines met the activity standard recommended by the World Health Organization for veterinary vaccines of this type.

[Testing for immunogenic and protective properties of vaccine against tissue-inactivated rabies]. / Overování imunogenních a protekcních schopností vakcíny proti vztekline tkánové inaktivované.

The immunogenic and protective potency was tested of vaccine against inactivated tissue rabies developed from the Vnukovo-32 strain, and produced in the Bioveta state corporation at Ivanovice in Haná. In 21 days after an i. m. application of 3 cm3 of vaccine, the average titre of virus-neutralizing antibodies was found to be 1:47. In this period the animals were revaccinated in the same way. The average titre of virus-neutralizing antibodies was 1:99 three months after revaccination, 1:57 in six months and 1:24 in nine months. In this period a challenge test was performed in a dog using the dose of 10(5) MICLD50 of street rabies virus. This dose was implanted i. m. into masticatory muscles. Another dog was infected experimentally 18 months after immunization. The experiment has proved the good immunogenic potency of vaccine against inactivated tissue rabies and its ability to induce the protection of vaccinated dogs from the strees infection with street rabies virus. The control rabies vaccine of foreign make RABISIN was tested in a similar way.