RESUMO
Optical biosensing is being actively investigated for minimally-invasive monitoring of key biomarkers both in vitro and in vivo. However, typical benchtop instruments are not portable and are not well suited to high-throughput, real-time analysis. This paper presents a versatile multichannel instrument for measurement of emission intensity and lifetime values arising from luminescent biosensor materials. A detailed design description of the opto-electronic hardware as well as the control software is provided, elaborating a flexible, user-configurable system that may be customized or duplicated for a wide range of applications. This article presents experimental measurements that prove the in vitro and in vivo functionality of the system. Such tools may be adopted for many research and development purposes, including evaluation of new biosensor materials, and may also serve as prototypes for future miniaturized handheld or wearable devices.
RESUMO
This paper presents a real-time functional decomposition adaptive algorithm for the optimal sampling of the interferometric signal in Swept-Source Optical Coherence Tomography imaging systems, which completely eliminates the input signal dependent nonlinearities that are problematic in current state-of-the-art OCT realizations that use interpolation and resampling. The proposed adaptive calibration algorithm uses the Kalman approach to estimate the wavenumber index parameter k from the Mach-Zender Interferometer signal which is then applied to an adaptive level crossing sampler to generate a sampling clock that k-linearizes the data on real-time during the sampling process. Such a system implements an artifact-free realization of the technology removing the need for classical interpolation and resampling. The new real-time linearization scheme has the additional capability of increasing the imaging acquisition speed by 10X while providing robustness to noise, properties that are demonstrated through mathematical analysis and simulation results throughout the paper.
