Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 7 de 7
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Rocz Panstw Zakl Hig ; 65(3): 185-91, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25247797

RESUMO

BACKGROUND: The European Union prohibits the use of chloramphenicol (CAP) as a veterinary drug in food-producing animals. Nevertheless, CAP have been detected in milk products (liquid milk and milk powder). Therefore, it is necessary to develop sensitive methods for determining CAP residues in milk powder. OBJECTIVE: The aim of this study was to develop and validate a confirmatory method for determination of CAP in milk powder. MATERIAL AND METHODS: Chloramphenicol was determined in milk powder using LC-ESI-MS/MS in negative mode. After fat removing milk powder sample was extracted/cleaned-up with a Chem Elut extraction cartridge. Separation was achieved on a Phenomenex Luna C-18 column with acetonitrile-water as a mobile phase. The mass spectrometer was operated in multiple reaction monitoring mode (MRM). Four transitions were monitored m/z 321→152, 321→194, 321→257 (CAP) and 326→157 (IS CAP-d5). RESULTS: Linearity, accuracy, precision, decision limit (CCa), detection capability (CCb) and ruggedness were determined for m/z 321→152. The mean relative recoveries (inter standard-corrected) of CAP from whole milk powder spiked at levels 0.1, 0.2, 0.3 and 0.6 mg/kg were in the range 95 - 103%. Relative standard deviation (RSD%) of recoveries at all spiked levels were less than 14%. RSDs within-laboratory reproducibility calculated at fortification of 0.3 mg/kg was less than 16%. CCa and CCb were below 0.1 mg/kg. CONCLUSIONS: The developed LC-MS/MS method allows the determination of CAP in milk powder. The method was validated according to the Commission Decision No. 2002/657/EC requirements. This method can be applied to determination CAP in whole and skim milk powder.


Assuntos
Cloranfenicol/análise , Contaminação de Alimentos/análise , Leite/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Animais , Cromatografia Líquida/métodos , Humanos , Pós , Espectrometria de Massas em Tandem/métodos
2.
Rocz Panstw Zakl Hig ; 64(4): 285-91, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-24693713

RESUMO

BACKGROUND: The use of nitrofurans as veterinary drugs in food-producing animals is banned throughout the European Union. Nevertheless, nitrofuran metabolites have been detected not only in animal products, but also in bovine urine. At present there are no methods yet published for the simultaneous detection of nitrofuran metabolites in bovine urine. OBJECTIVES: To develop and validate a method for determination of four key nitrofuran metabolites in bovine urine. MATERIAL AND METHODS: The four nitrofuran metabolites (nitrofurantoin, furazolidone, nitrofurazone and furaltadone), were determined in bovine urine using LC-ESI-MS/MS. The procedure required an acid-catalysed release of protein-bound metabolites, followed by their in situ conversion into 2-nitrobenzaldehyde (NBA) derivatives. The sample clean-up was performed using a polymer extraction cartridge before hydrolysis. Nitrofuran metabolites were then determined using electrospray ionization in the positive mode, that had previously been separated on a Phenomenex Luna C-18 column. RESULTS: The method was validated in accordance with the procedure outlined in the Commission Decision No. 2002/657/ EC. Urine samples were spiked with nitrofuran metabolite solutions at levels of 0.5, 1.0, 1.5 and 2.0 microg/kg. Recoveries ranged between 90 - 108% (inter standard-corrected), with a repeatability precision (RSD) of less than 19% for all four analytes. The decision limit (CC) and detection capability (DC) were obtained from a calibration curve and lay respectively within the following ranges: 0.11 - 0.34 microg/kg and 0.13 - 0.43 microg/kg. CONCLUSIONS: The developed and validated LC-ESI-MS/MS method allows four nitrofuran metabolites to be identified and quantitated in bovine urine. This analytical procedure meets the criteria defined in the Commission Decision No. 2002/657/EC.


Assuntos
Bovinos/urina , Cromatografia Líquida de Alta Pressão/veterinária , Nitrofuranos/urina , Espectrometria de Massas em Tandem/veterinária , Drogas Veterinárias/urina , Animais , Calibragem , Bovinos/metabolismo , Cromatografia Líquida de Alta Pressão/métodos , Nitrofuranos/análise , Espectrometria de Massas em Tandem/métodos , Urina/química , Drogas Veterinárias/análise
3.
Rocz Panstw Zakl Hig ; 61(3): 249-52, 2010.
Artigo em Polonês | MEDLINE | ID: mdl-21365859

RESUMO

The method is presented to analyze chloramphenicol in milk powder. Sample was clean-up by used molecular imprinted polymers (MIP). The determination was performed by LC-ESI-MS/MS. The LC was equipped with column Luna C18 Phenomenex. CAP-d5 was used as internal standards. The method was validation according to the criteria of Decision Commission No 2002/657/EC. Recoveries for the level 0.3 microg/kg was in the range 104-111%. The limit of decision (CCalpha) and detection capability (CCbeta) was respectively 0.06 microg/kg and 0.09 microg/kg.


Assuntos
Antibacterianos/análise , Cloranfenicol/análise , Contaminação de Alimentos/análise , Leite/química , Animais , Cromatografia Gasosa/métodos , Cromatografia Líquida , Humanos , Impressão Molecular/métodos , Polônia
4.
Rocz Panstw Zakl Hig ; 60(1): 19-23, 2009.
Artigo em Polonês | MEDLINE | ID: mdl-19579764

RESUMO

The method is presented to analyze azaperone and carazolol in pigs kidney. Samples were extracted with acetonitryle. The determination was performed by LC-ESI-MS/MS. The LC was equipped with column Luna C18 Phenomenex. Haloperidol was used as internal standards. The method was validation according to the criteria of Decision Commission No 2002/657/EC. Recoveries for the level 100 microg/kg azaperone and 25 microg/kg carazolol were in the range 91.2-107.0% and 70.8-93.2%. The limit of decision (CCalpha) and detection capability (CCbeta) was respectively 125.9 microg/kg, 160.0 microg/kg for azaperone and 29.3 microg/kg, 33.3 microg/kg. LC-MS/MS technique fulfill the requirements of Decision Commission No 2002/657/EC.


Assuntos
Antipsicóticos/análise , Azaperona/análise , Resíduos de Drogas/análise , Rim/química , Carne/análise , Propanolaminas/análise , Animais , Contaminação de Alimentos/análise , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Espectrometria de Massas por Ionização por Electrospray , Suínos
5.
Rocz Panstw Zakl Hig ; 58(3): 503-8, 2007.
Artigo em Polonês | MEDLINE | ID: mdl-18246654

RESUMO

Chloropromazina (CP) is subjected to monitoring food animals products, with a minimum required performance limit (MPRL) set 5.0 microg/kg. Homogenized kidney and urine were extracted with acetonitrile. CP- d3 was used as internal standard. LC separation was done on Luna C18 150 x 2 mm, 5 microm column in mobile phase acetonitrile-acetic acid. CP was determination by LC-ESI-MS/MS negative mode. The method was validation according to the criteria of Decision Commission No 2002/657/EC. Recoveries for the level 5.0 ng/g were in the range 84-102%. The limit of decision (CCalpha) and detection capability (CCbeta) CP in kidney were 1.19; 2.87 ng/g and urine 1.08; 2.61 ng/g.


Assuntos
Antibacterianos/análise , Antibacterianos/urina , Cloranfenicol/análise , Cloranfenicol/urina , Resíduos de Drogas/análise , Rim/química , Carne/análise , Animais , Contaminação de Alimentos/análise , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Espectrometria de Massas por Ionização por Electrospray
6.
Rocz Panstw Zakl Hig ; 58(4): 625-32, 2007.
Artigo em Polonês | MEDLINE | ID: mdl-18578344

RESUMO

A LC-MS-MS method is presented to analyze simultaneously the metabolites of four nitrofuran veterinary drugs in animal muscle tissue e.g., furazolidone, furaltadone, nitofurantoina and nitrofurazone. The sample clean up were performed by a liquid-liquid extraction with ethyl acetate, after a hydrolysis and derivatization with 2-nitrobenzaldehyde. Nitrofurane metabolites were determined by LC-ESI-MS/MS in positive mode. The LC was equipped with column Luna C18 Phenomenex. A binary gradient mobile phase was used as methanol solvent B containing 0.5 mM ammonium acetate and methanol (80:20 v/v). The method was validated according to criteria of Decision Commission No 2002/657/EC. Samples were fortified with metabolites of nitrofuran between 0.5-2.0 microg/kg with AOZ-d4, and AMOZ-d5 as internal standard. The mean recoveries from meat spiked at 1.0 microg/kg were 84.5-109.7%. Limit of decision (CCalpha) was between 0.25-0.57 and capability of detection (CCbeta) 0.32-0.77 microg/kg.


Assuntos
Cromatografia Líquida/métodos , Análise de Alimentos/métodos , Leite/química , Nitrofuranos/análise , Espectrometria de Massas por Ionização por Electrospray/métodos , Espectrometria de Massas em Tandem/métodos , Drogas Veterinárias/análise , Animais , Controle de Qualidade , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
7.
Rocz Panstw Zakl Hig ; 57(1): 31-7, 2006.
Artigo em Polonês | MEDLINE | ID: mdl-16900861

RESUMO

A liquid chromatographic method with mass spectrometric determination and identification (LC-MS/MS) for the determination of chloramphenicol (CAP) in tissues of animal origin was presented. Homogenized samples were extracted with ethyl acetate and evaporated to dryness followed by a clean-up using the liquid-liquid distribution between acetonitryle and hexane. Chloramphenicol was determined by LC-MS-ESI-MS in negative mode. There was used Phenomenex column with the mixture of acetonitrile-water as a mobile phase. The method was validated according to the criteria of Decision Commission No 2002/657/EC. Samples were fortified at CAP levels between 0.1 and 0.45 ng/g with 5d-CAP as internal standard. Recoveries for the level 0.3 ng/g were in the range 76.3-100.3%. Limit of decision (CCalpha) was between 0.137-0.205 ng/g. Limit of quantification (LOQ)--0.1 ng/g.


Assuntos
Antibacterianos/análise , Cloranfenicol/análise , Carne/análise , Animais , Contaminação de Alimentos/análise , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Espectrometria de Massas por Ionização por Electrospray
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...