Bioremediation of quinoline-contaminated soil using bioaugmentation in slurry-phase reactor / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate the possibility of using bioaugmentation as a strategy for remediating quinoline-contaminated soil.</p><p><b>METHODS</b>Microorganisms were introduced to the soil to assess the feasibility of enhancing the removal of quinoline from quinoline-contaminated soil. Slurry-phase reactor was used to investigate the bioremediation of quinoline-contaminated soil. HPLC (Hewlett-Packard model 5050 with an UV detector) was used for analysis of quinoline concentration.</p><p><b>RESULTS</b>The biodegradation rate of quinoline was increased through the introduction of Burkholderia pickettii. Quinoline, at a concentration of 1 mg/g soil, could be removed completely within 6 and 8 hours with and without combined effect of indigenous microbes, respectively. Although the indigenous microbes alone had no quinoline-degrading ability, they cooperated with the introduced quinoline-degrader to remove quinoline more quickly than the introduced microbes alone. Bioaugmentaion process was accelerated by the increase of inoculum size and bio-stimulation. The ratio of water to soil in slurry had no significant impact on bioremediation results.</p><p><b>CONCLUSION</b>Bioaugmetation is an effective way for bioremediation of quinoline-contaminated soil.</p>

Microbial degradation of aniline by bacterial consortium / 生物医学与环境科学（英文）

<p><b>OBJECTIVE</b>To investigate the characteristics of microbial degradation of aniline by a stable bacterial consortium.</p><p><b>METHODS</b>The bacterial consortium was isolated from activated sludge treating chemical wastewater using aniline as the sole source of carbon and nitrogen by enrichment and isolation technique. The biomass was measured as optical density (OD) at 510 nm using a spectrophotometer. Aniline concentrations were determined by spectrophotometer. The intermediates of aniline degradation were identified by GC/MS method.</p><p><b>RESULTS</b>The bacterial consortium could grow at a range of aniline concentrations between 50 and 500 mg/L. The optimal pH and temperature for aniline degradation were determined to be 7.0 and 30, respectively. The presence of NH4NO3 as an additional nitrogen source (100-500 mg/L) had no adverse effect on bacterial growth and aniline degradation. The presence of heavy metal ions, such as Co2+, Zn2+, Ni2+, Mn2+ and Cu2+ had an inhibitory effect on aniline degradation.</p><p><b>CONCLUSIONS</b>The isolated bacterial consortium can degrade aniline up to 500 mg/L effectively and tolerate some heavy metal ions that commonly exist in chemical wastewater. It has a potential to be applied in the practical treatment of aniline-containing wastewater.</p>