Clinical evaluation of a novel commercial multiplex-based PCR diagnostic test for differential diagnosis of dermatomycoses.

Dermatomycoses are very common worldwide with increasing prevalence. An accurate and rapid detection of fungi is most important for the choice of antimycotics and the success of treatment. The aim of this study was to evaluate a new commercial multiplex-based PCR which allows the detection and differentiation of the most relevant human pathogen fungi causing dermatomycoses in Europe. The accuracy and reproducibility of this application were verified in a clinical performance assessment in comparison to direct microscopy and culture using DNA isolates from 253 clinical samples. Sensitivity, specificity, positive predictive value and negative predictive value of 87.3%, 94.3%, 87.3% and 94.3%, respectively, were calculated for dermatophytes when confirmed by direct microscopy, culture or both. The corresponding values for Candida spp. were 62.7%, 93.5%, 77.8%, and 87.4%, respectively. Furthermore, in comparison to culture, the multiplex PCR was able to detect additional 38 Trichophytum rubrum and 12 Trichophytum interdigitale infections. These results were confirmed by independent PCR analysis. From DNA isolation to diagnosis the multiparameter diagnostic kit gives rise to a 1-day workflow, enables fast clarification of disease aetiology and, thus, contributes to specific therapy selection. The latter is particularly important in light of growing resistance to antimycotics.

Formation of electron holes and particle energization during magnetic reconnection.

Three-dimensional particle simulations of magnetic reconnection reveal the development of turbulence driven by intense electron beams that form near the magnetic x-line and separatrices. The turbulence collapses into localized three-dimensional nonlinear structures in which the electron density is depleted. The predicted structure of these electron holes compares favorably with satellite observations at Earth's magnetopause. The birth and death of these electron holes and their associated intense electric fields lead to strong electron scattering and energization, whose understanding is critical to explaining why magnetic explosions in space release energy so quickly and produce such a large number of energetic electrons.

Magnetic reconnection in toroidal eta(i) mode turbulence

Based on three-dimensional simulations of the Braginskii equations we show that for typical plasma-edge parameters the saturation of electromagnetic toroidal eta(i) mode turbulence is controlled by the self-generation and subsequent annihilation of radial magnetic field perturbations. This should be contrasted with the electrostatic limit, where the growth of the linear eta(i) mode is terminated by the onset of sheared flow modes driven by the radial plasma streams. The impact of the saturation amplitude on the transport level is substantial and is not in accord with simple mixing length arguments, suggesting that electromagnetic effects should generally be included in simulations of eta(i) mode turbulence.