RESUMO
Glucosylthiazolidine-4-carbonic acid, an intermediate of the Maillard reaction between D-glucose and L-cysteine, was given in doses of 0, 25, 50 or 100 mg/kg b.w. by oral intubation to male and female rats for 21 days. General appearance, growth, food consumption, haematology, urine analysis and serum chemistry including determinations of enzyme activities, organ weights and macroscopic and microscopic pathology were used as criteria for adverse effects. Effects on the kidneys were indicated by oliguria and decreased specific gravity of the urine in males and histopathological changes of the proximal tubules in females. The no-effect dose established from this study is 25 mg/kg b.w.
Assuntos
Glucosídeos/toxicidade , Rim/efeitos dos fármacos , Reação de Maillard , Tiazóis/toxicidade , Animais , Contagem de Células Sanguíneas/efeitos dos fármacos , Análise Química do Sangue , Relação Dose-Resposta a Droga , Ingestão de Alimentos/efeitos dos fármacos , Feminino , Masculino , Tamanho do Órgão/efeitos dos fármacos , Ratos , Urina , Aumento de Peso/efeitos dos fármacosRESUMO
ESR spectroscopic investigations on the mechanism of Maillard-reactions using the model D-glucose/glycine show radical formation in some of the reaction steps. These are possibly due to competitive reactions against ionic mechanism. The formation of radicals depends on the concentration of reactants and reaches a maximum in thermically induced browning reactions. The addition of L-cysteine leads to a decrease in the radical concentration to about 60%. It can be indirectly proven that the radicals are involved in the formation of browning products. The hyperfine structures of ESR spectra are supposed to be similar to pyrazine cation radicals.