Breast size in lactating women and the content of macronutrients in human milk.

INTRODUCTION: Although the primary function of a woman's breast is milk synthesis, only a few studies have evaluated the relationship between breast size and human milk composition, showing equivocal results. This study aims to test if breast size during fully established lactation is related to energy density and content of macronutrients in human milk. MATERIALS AND METHODS: Mothers of healthy, born-on-term infants at stage III of lactogenesis (N = 137) provided breast milk samples. Milk composition was analyzed using midinfrared transmission spectroscopy. The breast size index was calculated by dividing the breast by the circumference below the breast. RESULTS: Stepwise forward linear regression showed a negative association between breast size index and lactose concentration in breast milk (ß = -.242, p = .003). The final model, which includes breast size index, feeding frequency, and maternal energy intake together explained around 13% of the variance in breast milk lactose content (R2 adj = .126, p < .001). No statistically significant relationship was found between breast size index and milk's energy density, protein, and fat content. CONCLUSION: This is the first study that shows a negative relationship between breast size during fully established lactation and lactose concentration in milk in a large sample size. No relationship between other macronutrients and breast size indicates that large breast size is not necessary for adequate milk production; however, it may contribute to an altered lactose concentration.

Birth size and the serum level of biological age markers in men.

Previous studies showed that intrauterine growth restrictions, resulting in smaller body size at birth, are associated with altered development and the risk of age-related diseases in adult life. Thus, prenatal development may predict aging trajectories in humans. The study aimed to verify if body size at birth is related to biological age in adult men. The study sample consisted of 159 healthy, non-smoking men with a mean age of 35.24 (SD 3.44) years. Birth weight and length were taken from medical records. The ponderal index at birth was calculated. Biological age was evaluated based on serum levels of s-Klotho, hsCRP, DHEA/S, and oxidative stress markers. Pregnancy age at birth, lifestyle, weight, cortisol, and testosterone levels were controlled. The results showed no relationship between birth size and s-Klotho, DHEA/S level, inflammation, or oxidative stress. Also, men born as small-for-gestational-age (N = 49) and men born as appropriate-for-gestational-age (N = 110) did not differ in terms of biological age markers levels. The results were similar when controlled for pregnancy week at birth, chronological age, BMI, testosterone, or cortisol level. The results suggest that there is no relationship between intrauterine growth and biomarkers of aging in men aged 30-45 years from the affluent population.

Effects of Dominance and Sprint Interval Exercise on Testosterone and Cortisol Levels in Strength-, Endurance-, and Non-Training Men.

The aim of the study was to investigate the response of testosterone and cortisol to sprint interval exercises (SIEs) and to determine the role of dominance. The experiment was conducted in a group of 96 men, divided into endurance-training, strength-training, and non-training groups. Participants performed SIEs consisting of 5 × 10-s all-out bouts with a 50-s active recovery. Using the passive drool method, testosterone and cortisol concentrations were measured in saliva samples at rest at 10 min pre and 12 min post exercise. Participants' heart rate (HR) was measured during the whole exercise. Dominance was assessed by the participants before the study; the rating of perceived exertion (RPE) was measured immediately after each bout. The study showed that those who trained in endurance and strength sports had significantly lower mean HRs after five acute 10-s interval bouts than those in the non-training group (p = 0.006 and p = 0.041, respectively). Dominance has an inverse relation to changes in HR; however, it has no relation to hormone response. No significant differences were observed in testosterone and cortisol changes in the endurance-training, strength-training, and non-training groups after SIE (p > 0.05), which may indicate that the exercise volume was too low.

Perceived facial age and biochemical indicators of glycemia in adult men and women.

Glycemia is linked with one of the key mechanisms underlying the aging process and inter-individual differences in biological age. Previous research showed that glucose level is linked with perceived age in elder individuals. This study aimed to verify if glycemia is related to perceived facial age in healthy adult individuals as interventions in younger and healthy cohorts are crucial for preventing the onset of age-related diseases. The study sample consisted of 116 healthy men of mean age 35.53 ± 3.54 years (29.95-44.29) and 163 healthy women of mean age 28.38 ± 2.40 (24.25-34.17) years. Glycemia was evaluated by fasting glucose, insulin, HOMA-IR, and glycated hemoglobin level. BMI, facial sexual dimorphism, estradiol, testosterone, and hsCRP levels were controlled. Perceived age was evaluated based on standardized facial photos in an online survey. Additionally perceived facial aging was calculated as a difference between perceived age and chronological age. No relationship between the levels of biochemical indicators of glycemia and perceived facial age or aging was found both in men and women, also when controlled for possible confounders. This study shows that perceived facial age in adult individuals is rather linked with body adiposity of sexual dimorphism but not with glycemic markers.

S-Klotho level and physiological markers of cardiometabolic risk in healthy adult men.

S-Klotho is perceived as a biomarker of healthy aging that has been shown to be inversely associated with cardiometabolic risk in elderly individuals. The aim of this study was to test if s-Klotho level is associated with cardiometabolic risk markers in younger healthy men in order to verify the possible role of s-Klotho level as an early marker of cardiometabolic risk. A cross-sectional study was conducted among 186 healthy men (Mage=35.33, SDage=3.47) from a Western urban population. Serum basal levels of s-Klotho, lipid profile, homocysteine, glycemia markers, C-reactive protein, liver transaminases and creatinine were evaluated. Also, blood pressure was measured and cardiometabolic risk score and homeostatic model assessment for insulin resistance (HOMA-IR) were calculated. Testosterone and cortisol levels, self-reported psychological stress, physical activity, smoking in the past, alcohol use and body adiposity were controlled for. We found no relationship between levels of s-Klotho and physiological markers of cardiometabolic risk in the studied population. The results were similar when controlled for adiposity, testosterone level, physical activity, alcohol use and smoking in the past. We suggest that s-Klotho level is not an early marker of cardiometabolic risk in younger middle-aged healthy men.

Are Beards Honest Signals of Male Dominance and Testosterone?

The male beard is one of the most visually salient and sexually dimorphic traits and a hypothesized potential marker of other traits, such as dominance, masculinity, social status, and self-confidence. However, as men can easily alter their facial hair, beards may provide unreliable information about the beard owner's characteristics. Here, we examined whether beards are honest signals of biological (testosterone levels) and psychological (self-reported dominance) traits. Young (M = 21.29, SD = 1.54) and healthy men (N = 97) participated in the study. Their beards were measured directly (using digital calipers) and by self-report. Participants provided saliva samples before and after acute exercise (to assess their testosterone and cortisol levels) and reported their dominance on a 5-item scale. The results showed that beard length (directly measured and self-reported) was not related to testosterone levels or dominance; thus, no evidence was found to support the hypothesis that beards are honest (or dishonest) signals of the beard owners' testosterone levels and dominance.

The evolution of perennially enlarged breasts in women: a critical review and a novel hypothesis.

The possession of permanent, adipose breasts in women is a uniquely human trait that develops during puberty, well in advance of the first pregnancy. The adaptive role and developmental pattern of this breast morphology, unusual among primates, remains an unresolved conundrum. The evolutionary origins of this trait have been the focus of many hypotheses, which variously suggest that breasts are a product of sexual selection or of natural selection due to their putative role in assisting in nursing or as a thermoregulatory organ. Alternative hypotheses assume that permanent breasts are a by-product of other evolutionary changes. We review and evaluate these hypotheses in the light of recent literature on breast morphology, physiology, phylogeny, ontogeny, sex differences, and genetics in order to highlight their strengths and flaws and to propose a coherent perspective and a new hypothesis on the evolutionary origins of perennially enlarged breasts in women. We propose that breasts appeared as early as Homo ergaster, originally as a by-product of other coincident evolutionary processes of adaptive significance. These included an increase in subcutaneous fat tissue (SFT) in response to the demands of thermoregulatory and energy storage, and of the ontogenetic development of the evolving brain. An increase in SFT triggered an increase in oestradiol levels (E2). An increase in meat in the diet of early Homo allowed for further hormonal changes, such as greater dehydroepiandrosterone (DHEA/S) synthesis, which were crucial for brain evolution. DHEA/S is also easily converted to E2 in E2-sensitive body parts, such as breasts and gluteofemoral regions, causing fat accumulation in these regions, enabling the evolution of perennially enlarged breasts. Furthermore, it is also plausible that after enlarged breasts appeared, they were co-opted for other functions, such as attracting mates and indicating biological condition. Finally, we argue that the multifold adaptive benefits of SFT increase and hormonal changes outweighed the possible costs of perennially enlarged breasts, enabling their further development.

Predicted reproductive longevity and women's facial attractiveness.

Physical attractiveness has been shown to reflect women's current fecundity level, allowing a man to choose a potentially more fertile partner in mate choice context. However, women vary not only in terms of fecundity level at reproductive age but also in reproductive longevity, both influencing a couple's long-term reproductive success. Thus, men should choose their potential partner not only based on cues of current fecundity but also on cues of reproductive longevity, and both may be reflected in women's appearance. In this study, we investigated if a woman's facial attractiveness at reproductive age reflects anti-Müllerian hormone (AMH) level, a hormone predictor of age at menopause, similarly as it reflects current fecundity level, estimated with estradiol level (E2). Face photographs of 183 healthy women (Mage = 28.49, SDage = 2.38), recruited between 2nd - 4th day of the menstrual cycle, were assessed by men in terms of attractiveness. Women's health status was evaluated based on C-reactive protein level and biochemical blood test. Serum AMH and E2 were measured. The results showed that facial attractiveness was negatively correlated with AMH level, a hormone indicator of expected age at menopause, and positively with E2, indicator of current fecundity level, also when controlled for potential covariates (testosterone, BMI, age). This might result from biological trade-off between high fecundity and the length of reproductive lifespan in women and greater adaptive importance of high fecundity at reproductive age compared to the length of reproductive lifespan.

Pharyngeal Detection of Staphylococcus aureus as a Possible Factor Related to Disgust Sensitivity in Humans.

Disgust triggers behavioral avoidance of pathogen-carrying and fitness-reducing agents. However, because of the cost involved, disgust sensitivity should be flexible, varying as a function of an individual's immunity. Asymptomatic colonization with Staphylococcus aureus often results from weakened immunity and is a potential source of subsequent infections. In this study, we tested if pharyngeal colonization with S. aureus, evaluated based on a single swab collection, is related to an individual's disgust sensitivity, measured with the Three Domain Disgust Scale. Levels of immunomodulating hormones (cortisol and testosterone), general health, and body adiposity were controlled. Women (N = 95), compared to men (N = 137), displayed higher sexual disgust sensitivity, but the difference between individuals with S. aureus and without S. aureus was significant only in men, providing support for prophylactic hypothesis, explaining inter-individual differences in disgust sensitivity. Men (but not women) burdened with asymptomatic S. aureus presence in pharynx exhibit higher pathogen disgust (p = 0.04) compared to individuals in which S. aureus was not detected. The positive relationship between the presence of the pathogen and sexual disgust was close to the statistical significance level (p = 0.06), and S. aureus colonization was not related with moral disgust domain.

Difference in Perception of Onset of Old Age in Traditional (Hadza) and Modern (Polish) Societies.

Despite relatively clear physiological indicators of old age, little is known about cross-cultural differences in psychological perceptions of the transition to old age. Although recent studies suggest consistency between modern countries, the subjective perception of old age onset in traditional societies remains poorly explored. Therefore, we compared the perception of timing of old age between a traditional tribe of hunter-gatherers (the Hadza) and a Polish sample representing a modern, industrialized population. The results indicate that the Hadza perceive old age onset as being significantly earlier than do the Poles. Furthermore, we found between-gender differences in the Polish sample: men set a lower threshold of old age onset than women. The Hadza showed no between-gender difference. Although the samples were matched for age, a larger proportion of Hadza considered themselves old. We discuss these findings from cultural and demographical perspectives.

Birth size and morphological femininity in adult women.

BACKGROUND: Women's morphological femininity is perceived to develop under the influence of sex hormones and to serve as a cue of estradiol level, fertility and health in mating context. However, as the studies on direct relationship between femininity and sex steroid levels have reported mixed results, it is still not well understood what factors contribute to inter-women variation in morphological femininity. Epidemiological studies show that indicators of adverse conditions during intrauterine growth and development in utero, such as low birthweight or relative thinness at birth, influence women's physiology ovarian functioning and may be associated with life-time exposure to estradiol in women. Thus, here we tested if birth parameters are also related with the level of morphological femininity in adult women. RESULTS: One hundred sixty-five healthy women of mean age 28.47 years (SD = 2.39) participated in the study. Facial femininity was estimated based on facial width-to-height ratio (fWHR) and facial shape sexual dimorphism measured in the photos. Body femininity was estimated based on waist-to-hip ratio (WHR) and breast size. Birth weight and birth length were obtained from medical records and ponderal index at birth was calculated. No relationship between birth parameters and facial or body femininity in women of reproductive age was found, also when controlled for adult sex steroid levels and BMI. CONCLUSIONS: The results suggest that, although previous research showed that birth parameters predict reproductive development and adult oestradiol level, they do not explain the variance in morphological femininity in women of reproductive age, trait that is thought to be a cue of a woman's estradiol level and fertility in mating context.

Do adipokines levels influence facial attractiveness of young women?

OBJECTIVES: Facial attractiveness is thought to reflect an individual's biological condition. This seems to be largely explained by the relationship between facial appearance and body adiposity, a trait that affects various aspects of body homeostasis, including fertility and immunity. The aim of this study was to test if, a part of adipose tissue amount, also levels of hormones secreted by adipose tissue are reflected in women's appearance, focusing on the two most abundant adipokines. Due to the opposing effects of adiponectin and leptin on health, we hypothesized that leptin negatively and adiponectin positively correlate with women's attractiveness. METHODS: The study sample included 174 young, healthy women (Mage = 28.50, SDage = 2.38). Serum leptin and adiponectin levels were measured. Estradiol (E2), testosterone (T), and BMI levels were controlled in the analyses. Face photographs were taken and facial attractiveness ratings, assessed by men, were gathered in online questionnaires. RESULTS: Perceived facial attractiveness correlated negatively with leptin level and leptin/adiponectin ratio, but did not correlate with adiponectin level. The results were similar, when controlled for E2, T, and BMI. Adipokines levels did not mediate or moderate the relationship between facial attractiveness and BMI. CONCLUSIONS: The results showed that perceived facial attractiveness is predicted by adipose-derived hormones detrimental for health, like leptin, but is not related with beneficial hormones, such as adiponectin. However, the levels of these two adipokines do not impact the relationship between perceived facial attractiveness and adiposity, and thus do not explain the relationship between facial attractiveness, body adiposity, and biological condition.

Facial appearance and metabolic health biomarkers in women.

Facial appearance has been suggested to provide an honest cue of an individual's biological condition. However, there is little direct evidence that facial attractiveness reflects actual health. Here we tested if facial appearance is related with metabolic health biomarkers. Face photographs of 161 healthy, young women (Mage = 28.59, SDage = 2.34) were assessed in terms of perceived attractiveness and health. Metabolic health was evaluated based on levels of markers of lipid and glucose metabolism balance, liver functioning, and inflammation. BMI, testosterone (T), and estradiol (E2) levels were controlled. Facial attractiveness, but not health, was negatively related with lipid profile components detrimental to health (total cholesterol, LDL, triglycerides) but not with relatively protective for health HDL. When controlled for BMI, E2, and T, only the relationship between attractiveness and triglycerides remained significant. Facial appearance was unrelated with glucose metabolism, liver functioning, and inflammatory markers. The results suggest, that for healthy women of reproductive age, such measures as BMI and sex hormone levels may be better predictors of attractiveness, compared to measures of metabolic health. Markers of lipid, glucose homeostasis, liver functioning or low-grade inflammation may be rather indicators of future health, of lesser importance in mating context, thus only modestly reflected in facial appearance.

No relationship between the digit ratios (2D:4D) and salivary testosterone change: Study on men under an acute exercise.

The digit ratio (2D:4D) is said to be a potential marker of exposure to prenatal sex steroids. Some studies suggest that the 2D:4D is also linked with the testosterone response to challenging situations due to organizational effect of prenatal hormonal milieu on adult endocrine functioning. However, up to date, there were only four studies (conducted on small samples) that examined the 2D:4D and the testosterone response to a challenging situation (i.e. physical exertion or aggressive context). Here, we examined the relationship between the 2D:4D and the testosterone change under an acute exercise among 97 men. We found that the digit ratios (the right 2D:4D, the left 2D:4D, and the right minus left 2D:4D) were neither predictors of pre-exercise testosterone, nor the change in testosterone level after a cycling task. Our results add a contradictory to previous studies evidence in a discussion on the links of the 2D:4D and the testosterone change.

Romantic Love and Reproductive Hormones in Women.

Increased reproductive success is among the most commonly proposed adaptive functions of romantic love. Here, we tested if hormonal changes associated with falling in love may co-vary with hormonal profiles that predict increased fecundity in women. We compared blood serum levels of estradiol (E2, E2/T), luteinizing hormone (LH), follicle-stimulating hormone (FSH), prolactin (PRL), free testosterone (fT), and cortisol (CT), measured in the early follicular phase of the menstrual cycle in single women (N = 69) and in women at the beginning of a romantic heterosexual relationship who reported being in love with their partner (N = 47). Participants were healthy, regularly cycling women aged 24 to 33 who did not use hormonal contraception. We found that women in love had higher levels of gonadotropins (FSH, LH) and lower testosterone levels compared to single women who were not in love. These groups of women did not, however, differ in terms of estradiol, prolactin, or cortisol levels.

National income inequality predicts cultural variation in mouth to mouth kissing.

Romantic mouth-to-mouth kissing is culturally widespread, although not a human universal, and may play a functional role in assessing partner health and maintaining long-term pair bonds. Use and appreciation of kissing may therefore vary according to whether the environment places a premium on good health and partner investment. Here, we test for cultural variation (13 countries from six continents) in these behaviours/attitudes according to national health (historical pathogen prevalence) and both absolute (GDP) and relative wealth (GINI). Our data reveal that kissing is valued more in established relationships than it is valued during courtship. Also, consistent with the pair bonding hypothesis of the function of romantic kissing, relative poverty (income inequality) predicts frequency of kissing across romantic relationships. When aggregated, the predicted relationship between income inequality and kissing frequency (r = 0.67, BCa 95% CI[0.32,0.89]) was over five times the size of the null correlations between income inequality and frequency of hugging/cuddling and sex. As social complexity requires monitoring resource competition among large groups and predicts kissing prevalence in remote societies, this gesture may be important in the maintenance of long-term pair bonds in specific environments.

Maternal breast volume in pregnancy and lactation capacity.

OBJECTIVES: Successful breastfeeding, crucial for a child's development, depends on a woman's ability to initiate lactation, milk yield, and composition. Those traits differ among women, but the cause and physiological mechanisms responsible for this variation are not fully understood yet. Growing evidence shows that lactation and milk composition vary among women and depend on maternal traits. The aim of this study was to test whether breast volume and its changes during pregnancy are related to the nutritional quality of breastmilk and to breastfeeding dynamics. MATERIAL AND METHODS: Almost 93 pregnant women (mean age: 29.67 ± 3.65), participated in the longitudinal study. Breast measurement was performed at each pregnancy trimester using 3D scanning. Milk samples were collected in the beginning of the second month of lactation from 69 women. Milk macronutrient and calorie content were measured. Women were also interviewed on breastfeeding time and frequency. RESULTS: Breast volume changes during pregnancy were not related to the probability of establishing successful lactation, milk calorie, fat and protein content. We found a negative correlation between milk lactose content and breast volume in the second and third trimester but not with breast volume change. Maternal breast volume in pregnancy was also positively related to the total breastfeeding time (per 24 hr). CONCLUSIONS: The results are in line with the notion that milk macronutrient content tends to be weakly associated with various maternal factors and adverse conditions. However, maternal breast volume and its changes during pregnancy are related with time spent breastfeeding per day, what suggests a positive correlation between breast volume in pregnancy and milk yield.

Body symmetry and reproductive hormone levels in women.

Fluctuating asymmetry (FA), a morphological marker of developmental stability, may be related to an individual's biological condition, e.g., health or fertility. The aim of this study was to test if the level of a woman's FA was related to her fertility and reproductive potential as measured by reproductive hormone levels. Fifty-three healthy, non-pregnant, naturally cycling women (mean age = 23.42, SD = 1.85 years), participated in the study, conducted in Wroclaw (Poland) in May 2015. Early-follicular phase serum levels of anti-Müllerian hormone (AMH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) were measured. FA was calculated based on anthropometric measures of six bilateral body traits, and the composite FA index was used in statistical analyses. No relationship was observed between FA and the levels of FSH, LH, and AMH (p > .05), controlled for potential confounders. However, the level of E2 was positively correlated with FA (p < .05). Thus, in young women, FA was not related to hormones levels related to ovarian reserve, but more symmetrical women had lower E2 levels. As FA is an index of developmental stability, environmental, and genetic stress, the results of the study confirm previous research suggesting that developmental conditions may be related to women's endogenous estrogen levels.

Maternal hand grip strength in pregnancy, newborn sex and birth weight.

AIM: Hand grip strength (HGS) is a non-invasive measure of physical strength, overall health, and nutritional status. The aim of the study was to test if HGS and its changes in pregnancy are related with offspring sex and birth weight. METHODS: We conducted longitudinal study on 95 healthy pregnant women (mean age 29.57 ±â€¯3.43). HGS was measured in each trimester. A woman's height and changes in weight in pregnancy were also measured. The information on a child's sex, birth weight and the pregnancy week at delivery were derived from hospital records. Fifty one women delivered a boy, and forty four women delivered a girl. RESULTS: HGS decreased from the first to the third trimester of pregnancy (F(2,188) = 15.94, p < 0.001). Women with greater HGS in each trimester were more likely to give birth to a boy (p < 0.05), and the decrease in HGS in pregnancy was comparable in the two groups of mothers (F(2,186) = 1.39, p = 0.25). HGS in pregnancy was related with offspring birth weight when controlled for a child's sex and week at delivery (F(2,182) = 3.15, p = 0.04). Maternal height also important predictor of HGS in pregnancy, and the decrease in HGS was only observed in shorter women. CONCLUSION: The results of this study indicate that HGS is a sensitive marker, differentiating the variation in physical condition in healthy, well-fed pregnant women in affluent population and pregnancy outcome (offspring sex and birth weight). Also, the result indicates that relatively taller women bear lower cost of pregnancy and are able to invest more in developing foetus.