RESUMO
OBJECTIVE: To define the features of prooxidant antioxidant balance in patients with nonalcoholic fatty liver disease who have been exposed to radiation as a result of the Chornobyl accident. METHODS: using Indicators characterizing the processes of oxidative modification of macromolecules by blood lev els of compounds with isolated double bonds (CIDB), diene conjugates (DC) oxodiene conjugates (ODC), secondary products of lipid peroxiodation reacting with thiobarbituric acid, products of oxidative modification of proteins in blood serum by levels of neutral and basic 2,4 dynitrofenilhidrazones, superoxide dismutase and catalase content in erythrocytes and ceruloplasmin in plasma were determined for the diagnosis of oxidative stress. The factor of antiox idant state was calculated. RESULTS: In the absence of differences between the ratios between the primary and intermediate products of lipids peroxidation increasing relative content of DC and ODC in patients with nonalcoholic steatohepatitis (NASH), which suffered radiation exposure, was found compared with patients without nonalcoholic fatty liver disease (NAFLD) and NASH patients, who had no history of radiation exposure. This may be a manifestation of insignificant activation of lipid peroxidation and disturbance of LPO products transformation at different stages. In irradiated steatohepato sis (SH) patients, unlike SH patients who have not undergone radiation exposure,, the proportionalityof lipid perox idation products between the content of izopropanol phase lipid extracts of blood was violated, and Spearman cor relation coefficients between CIDB and DC and DC and ODC did not exceed the limits average values. In the group of patients with SH who suffered from radiation exposure level CIDB was slightly higher compared with patients with NASH. Regardless of whether there was a history of radiation exposure, the level of oxidative protein modification (OMB) products in NASH patients was higher compared with patients with SH, but the difference reached significant values only in the subgroup of non irradiated patients with NASH by 2.4 dinitrophenilhydrazones of neutral charac ter. Mean values ??of catalase activity in patients with NASH also exceeded the corresponding values of SH patient. The average values of the factor of antioxidant status in patients with both SH and NASH subjected to radiation exposure, were lower than in the corresponding subgroups of non irradiated patients by 29 and 21%, respectively. CONCLUSION: Existing dismetabolic disorders may contribute to the development of endogenous intoxication and deepening of pathological changes in the liver of NAFLD patients from SH to NASH.
RESUMO
OBJECTIVE: To study the serum ferritin levels in children with acute leukemia, depending on the number of transfusions of RBC concentrate and period of disease. MATERIALS AND METHODS: We studied the red blood count, serum iron and ferritin levels in 54 patients with acute leukemia before chemotherapy, at the time of a standardized treatment protocol, and after transfusions of RBC concentrates. RESULTS: In the debute of acute leukemia just before treatment lauch the serum ferritin in 81.5% of children was 2.3-2.5 higher than normal. The need for transfusion of RBC concentrates was higher under serum ferritin level exceeding 500 ng/mL. The association was established between ferritin content and age of the children, variant of acute leukemia and period of the disease. CONCLUSIONS: The level of serum ferritin can be used as a marker of ferrokinetic status for timely diagnosis of iron overload in children with acute leukemias and for application of treatment-and-prophylactic actions.
