Analysis of the Surface of Historic Fabric from the Auschwitz-Birkenau State Museum after Treatment with Ethanol Mist Used to Eliminate Microorganisms Harmful to Human Health.

AIM: the aim of the work was to present the changes occurring on the model and historical cotton surface of cotton resulting from disinfection with 90% ethanol mist. MATERIALS AND METHODS: Samples of historical materials consisted of fabric elements from suitcases stored in A-BSM. A mist of 90% ethanol was applied for 15 s at a distance of 16 cm from the surface. The spectra of cotton samples before and after ethanol application were recorded using Fourier transform infrared spectroscopy (FTIR-ATR). Analyses of the surface layers were performed using X-ray photoelectron spectroscopy (XPS). RESULTS: the decontamination performed did not show any significant differences in the chemical composition and surface structure of cotton before and after the use of 90% ethanol mist. CONCLUSIONS: Ethanol mist, which eliminates microorganisms from the historical surface, does not cause significant changes to the surface of historical objects.

Analysis of the Microbiome on the Surface of Corroded Titanium Dental Implants in Patients with Periimplantitis and Diode Laser Irradiation as an Aid in the Implant Prosthetic Treatment: An Ex Vivo Study.

The paper presents the optimization of diode laser irradiation of corroded dental implants in order to reduce the number of microorganisms associated peri-implantitis. The research included the identification of microorganisms on the surface of removed dental implants in patients with peri-implantitis and the assessment of the biocidal effectiveness of the diode laser against these microorganisms. Laser desorption/mass spectrometry (MALDI-TOF MS) was used to identify microorganisms and metagens were examined by next generation sequencing (NGS). Irradiation was performed with a diode laser with a wavelength of λ = 810, operating mode: 25 W/15.000 Hz/10 µs, average = 3.84 W with the number of repetitions t = 2 × 15 s and t = 3 × 15 s. The structure and surface roughness of the implants were analysed before and after laser irradiation by optical profilometry and optical microscopy with confocal fixation. In total, 16 species of Gram-positive bacteria and 23 species of Gram-negative bacteria were identified on the surface of the implants. A total of 25 species of anaerobic bacteria and 12 species with corrosive potential were detected. After diode laser irradiation, the reduction in bacteria on the implants ranged from 88.85% to 100%, and the reduction in fungi from 87.75% to 96.77%. The reduction in microorganisms in the abutment was greater than in the endosseous fixture. The applied laser doses did not damage, but only cleaned the surface of the titanium implants. After 8 years of embedding, the removed titanium implant showed greater roughness than the 25-year-old implant, which was not exposed to direct influence of the oral cavity environment. The use of a diode laser in an optimised irradiation dose safely reduces the number of microorganisms identified on corroded dental implants in patients with peri-implantitis.