RESUMO
Genetic polymorphisms in the interleukin-6 (IL-6) gene are associated with bone homeostasis and diseases characterized by bone loss. The aim of this study was to investigate the correlation between the IL-6-572C/G polymorphism and the risk of chronic periodontitis in a Chinese Han population. The IL-6-572C/G polymorphism was genotyped in 93 patients suffering from chronic periodontitis and 96 control subjects by polymerase chain reaction-restriction fragment length polymorphism. DNA was extracted from the peripheral blood of patients and control subjects and amplified using a polymerase chain reaction. IL-6 genotypes were identified using gel electrophoresis. The IL-6-572 GG genotype and the G allele were more frequent in chronic periodontitis patients than in control subjects (P<.05). When compared with the CC genotype, the odds ratio for chronic periodontitis was 1.88 (95% confidence interval, 1.04 to 3.40; P<.05) for the CG+GG genotype. The frequency of the -572CG+GG genotype was significantly different between the control group and the group with chronic periodontitis. Therefore, the IL-6-572C/G polymorphism may contribute to the susceptibility to chronic periodontitis in the Chinese Han population.
Assuntos
Povo Asiático/genética , Periodontite Crônica/genética , Interleucina-6/genética , Adulto , Idoso , Alelos , Estudos de Casos e Controles , China , Feminino , Frequência do Gene , Predisposição Genética para Doença , Humanos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Polimorfismo de Fragmento de Restrição , Polimorfismo de Nucleotídeo ÚnicoRESUMO
<p><b>OBJECTIVE</b>The purposes of this study were to investigate effects of chloroqquine (CQ) on inhibiting the decomposition of hydrogen peroxides (HP), and to optimize composition of the tooth-bleaching agent.</p><p><b>METHODS</b>According to the principle of the color-changing reaction between horse radish peroxidase and substrate, the tooth-bleaching agent made of HP was divided into four groups with different amounts of CQ. The stability of HP was observed using ELISA for 3 months.</p><p><b>RESULTS</b>With the prolongation of store time, the absorbance of HP solutions containing different concentrations of CQ declined at different degrees, which showed a dependent relation between absorbance values and concentrations of CQ. Within the range of experiment concentrations of CQ, the higher the concentration of CQ was, the stronger the stability of HP was. And the duration of tooth-bleaching effects in 150.0 mg/ml of CQ was prolonged 4 to 6 times compared to that without CQ.</p><p><b>CONCLUSION</b>The data indicate that CQ can inhibit the decomposition of HP. The bleaching effect of the tooth-bleaching agent which is made of HP and proper amount of CQ is satisfactory.</p>
Assuntos
Humanos , Cloroquina , Química , Combinação de Medicamentos , Interações Medicamentosas , Peróxido de Hidrogênio , Química , Clareamento Dental , MétodosRESUMO
0.05).However, the frequency of the allele of g(-1359)t polymorphisms was higher in patients than that in the controls(P
