RESUMO
Abundant evidence now supports the idea that methionine deficiency has negative effects on chicken healthy especially in the aspect of cell cycle regulation and apoptosis. But, lacking of knowledge is the evaluation on metabolic organs. To test the effect of methionine deficiency on the kidney, we assessed the apoptosis and the cell cycle of kidney induced by methionine deficiency by the methods of TdT-mediated dUTP Nick-End Labeling (TUNEL), flow cytometry (FCM), quantitative real-time PCR (qRT-PCR) and Enzyme-Linked ImmunoSorbent Assay (ELISA) of chickens for 42â¯days of age. Our results showed that the number of the apoptotic cells was increased (pâ¯<â¯.05 or pâ¯<â¯.01), while bcl-2 mRNA expression levels were decreased (pâ¯<â¯.05 or pâ¯<â¯.01) and bax and caspase-3 mRNA expression levels were higher (pâ¯<â¯.01) in methionine deficiency group. Furthermore, the cell cycle results showed a time-dependent increase in G2M phase cells and a corresponding decrease in cells at G2M and S stages, the mRNA expression of p53 and p21 was increased (Pâ¯<â¯.05 or Pâ¯<â¯.01) and cyclin B and PCNA was significantly lower (Pâ¯<â¯.05 or Pâ¯<â¯.01) in the methionine deficiency group than that of the control group. These findings suggested that methionine deficiency could induce renal apoptosis and cell cycle arrest.
