Comparison on HPLC analysis of aerial part of Bupleurums from different germplasm resources / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate ingredient differences of the aerial part of Bupleurums from different germplasm resources by HPLC.</p><p><b>METHOD</b>Twenty-one different germplasm resources were sown and cultured at the same conditions and the aerial parts of the biennial plants were analyzed by HPLC.</p><p><b>RESULT</b>The chromatograms were established and the data were analyzed by Hierarchical Cluster Analysis to compare the similarity of the samples. The results showed that ingredients from various germplasm resources were significantly different, which indicated the germplasm diversity of Bupleurum.</p><p><b>CONCLUSION</b>The method can be used for comparison of the ingredient differences and quality control, the results provide important references for classification of Bupleurum species.</p>

[Effect of N, P, K on rhizome yield and diosgenin content of Dioscorea zingiberensis].

OBJECTIVE: A field experiment was conducted to investigate the different effects of various applying ratios of nitrogen (N), potassium (K) and phosphate (P) on diosgenin content and Rhizoma yield of Dioscorea zingiberensis. METHOD: Experiment was conducted with both one factor and orthogonal design, and data was analyzed statistically on SPSS 11. 5. RESULTS: Compared with the control groups,the yield of rhizome was significantly increased by N (P < 0.01), K (P < 0.01) , P (P <0.05). The content of diosgenin has no obvious increase (P > 0.05) by individual application of N, P and K, while changed significantly (P < 0.05) by the combination of proper levels of N, P, K. CONCLUSION: The degree of effect on diosgenin content was ranged with N > K > P, while on output of dioscorea rhizome was K > N > P, and the combination of N, K, P2 was superior to others.