Spatiotemporal Patterns of Reptile and Amphibian Road Fatalities in a Natura 2000 Area: A 12-Year Monitoring of the Lake Karla Mediterranean Wetland.

The pervasive expansion of human-engineered infrastructure, particularly roads, has fundamentally reshaped landscapes, profoundly affecting wildlife interactions. Wildlife-vehicle collisions, a common consequence of this intricate interplay, frequently result in fatalities, extending their detrimental impact within Protected Areas (PAs). Among the faunal groups most susceptible to road mortality, reptiles and amphibians stand at the forefront, highlighting the urgent need for global comprehensive mitigation strategies. In Greece, where road infrastructure expansion has encroached upon a significant portion of the nation's PAs, the plight of these road-vulnerable species demands immediate attention. To address this critical issue, we present a multifaceted and holistic approach to investigating and assessing the complex phenomenon of herpetofauna road mortality within the unique ecological context of the Lake Karla plain, a rehabilitated wetland complex within a PA. To unravel the intricacies of herpetofauna road mortality in the Lake Karla plain, we conducted a comprehensive 12-year investigation from 2008 to 2019. Employing a combination of statistical modeling and spatial analysis techniques, we aimed to identify the species most susceptible to these encounters, their temporal and seasonal variations, and the ecological determinants of their roadkill patterns. We documented a total of 340 roadkill incidents involving 14 herpetofauna species in the Lake Karla's plain, with reptiles, particularly snakes, being more susceptible, accounting for over 60% of roadkill occurrences. Moreover, we found that environmental and road-related factors play a crucial role in influencing roadkill incidents, while spatial analysis techniques, including Kernel Density Estimation, the Getis-Ord Gi*, and the Kernel Density Estimation plus methods revealed critical areas, particularly in the south-eastern region of Lake Karla's plain, offering guidance for targeted interventions to address both individual and collective risks associated with roadkill incidents.

An (Un)Expected Threat for a Regionally Near-Threatened Species: A Predation Case of a Persian Squirrel on an Insular Ecosystem.

One of the most successful predators on island ecosystems is the domestic cat, which is considered responsible for the decline of numerous species' populations. This can be estimated by the analysis of cats' dietary habits, yet prey identification is not always possible, and thus, in cases where precise prey identification is required, one of the most accurate methods derives from observing the hunting process. However, the cryptic nature of the feral cats and the constant vigilance of the species that are preyed upon make the observation process difficult, especially when the prey has a low population density. Here, we report for the first time such a case: a feral cat that has ambushed, killed, and consumed a regionally near-threatened species, the Persian squirrel. This incidental observation happened in the squirrel's westernmost end of its distribution, the island of Lesvos, Greece. Due to the unexpectedness of the event, in the following days, we estimated both the squirrels' and cats' population density. Results showed that while the density of the squirrels is moderate, the population density of the feral cats is almost fifteen times higher. For this reason, management actions need to be taken in an effort to minimize the impacts of feral cats on the native species of the island.