A Convenient Electrochemiluminescent Immunosensor for Detecting Methamphetamine Antibody.

An antibody-based immunotherapy for methamphetamine (MA) addictive treatment is has been drawing more and more attention in recent years. However, studies about methamphetamine antibody (anti-MA) immunodetections are rare, owing to the lack of immunogenicity of small molecule MA. This study provides a simple and effective approach to develop a convenient electrochemiluminescent (ECL) immunosensor for the testing of anti-MA. In short, the synthetic holoantigen of MA is immobilized on a homemade gold nanoparticles modified electrode as the sensing host for the specific recognition and detection of anti-MA. The research suggested, under optimal experimental conditions, the ECL intensity on resultant immunosensor has a wide-linear regression toward the anti-MA quantity within the range from 0.03 to 3.07 ng with a detection limit of 2.32 pg. It responded to the dosage of anti-MA in spiked blood samples with satisfactory recovery. According to the research, the developed sensor shows promise as a portable Anti-MA fast seized device which performs quickly and offers convenience, and will be helpful for forensic identification and clinical treatment.

A label-free genetic biosensor for diabetes based on AuNPs decorated ITO with electrochemiluminescent signaling.

The variation of I27L gene closely relates to increasing risk of type 2 diabetes, thus it is greatly significant to develop various methods for its identification or monitoring. We report here a novel label-free electrochemiluminescent (ECL) biosensor for simple and effective determination of I27L gene based on Au nanoparticles functionalized ITO electrode. The ECL technique is employed to monitor the hybridization of DNA strands by measuring the changes of its intensity. Here, the ECL signal was quenched by blocked access of probe (luminol anion) owing to the electrostatic repulsion of negatively charged sensor surface and the space resistance. The quantification of target strand can be directly realized by calibrating the quenched ECL signal toward its logarithm concentration in good linearity within the range from 1.0 × 10-11 to 1.0 × 10-7 M and a detection limit of 8.1 × 10-12 M. In addition, the biosensor exhibited good stability, excellent reproducibility and outstanding selectivity against one-base mismatched strand. What's more, the simple, low-cost, sensitive device could be easily miniaturized, makes it as an attractive candidate for integrating into portable platforms for point-of-care molecular diagnostics.