Motorized and non-motorized mixed traffic characteristics and lateral opening spacing calculation for the section of arterial highway through small towns.

A large number of motor vehicles and non-motorized vehicles mixed in the section of arterial highway crossing the town leads to many traffic problems. Therefore, it is necessary to set up a side divider between motorized and non-motorized lanes, and the appropriate spacing of lateral crossing openings to meet the needs of non-motorized vehicles crossing the highway has become a key issue that must be resolved. This paper investigates the traffic flow characteristics of mixed traffic flow on arterial highways through small town sections, and from the two dimensions of highway access efficiency and the psychological characteristics of cyclists, it calibrates the setting range of the spacing of non-motorized lateral crossing openings under different design speeds, which is used to regulate the behavior of non-motorized vehicles crossing the street, improve the safety level of the highway, reduce the lateral interference of the highway, and improve the road access efficiency. The accuracy of the research results is verified by microscopic simulation experiments, which proves that they can meet good expectations in practical engineering. The research results have theoretical significance and reference value for improving the status quo of machine-non-mixed traffic in the section of arterial highway passing through small towns, and enhancing the efficiency and safety of highway traffic. It also provides corresponding reference for the areas facing similar problems worldwide.

Setosphaeria turcica ATR turns off appressorium-mediated maize infection and triggers melanin-involved self-protection in response to genotoxic stress.

Eukaryotic organisms activate conserved signalling networks to maintain genomic stability in response to DNA genotoxic stresses. However, the coordination of this response pathway in fungal pathogens remains largely unknown. In the present study, we investigated the mechanism by which the northern corn leaf blight pathogen Setosphaeria turcica controls maize infection and activates self-protection pathways in response to DNA genotoxic insults. Appressorium-mediated maize infection by S. turcica was blocked by the S-phase checkpoint. This repression was dependent on the checkpoint central kinase Ataxia Telangiectasia and Rad3 related (ATR), as inhibition of ATR activity or knockdown of the ATR gene recovered appressorium formation in the presence of genotoxic reagents. ATR promoted melanin biosynthesis in S. turcica as a defence response to stress. The melanin biosynthesis genes StPKS and StLac2 were induced by the ATR-mediated S-phase checkpoint. The responses to DNA genotoxic stress were conserved in a wide range of phytopathogenic fungi, including Cochliobolus heterostrophus, Cochliobolus carbonum, Alternaria solani, and Alternaria kikuchiana, which are known causal agents for plant diseases. We propose that in response to genotoxic stress, phytopathogenic fungi including S. turcica activate an ATR-dependent pathway to suppress appressorium-mediated infection and induce melanin-related self-protection in addition to conserved responses in eukaryotes.

Whole Genome Analyses of Chinese Population and De Novo Assembly of A Northern Han Genome.

To unravel the genetic mechanisms of disease and physiological traits, it requires comprehensive sequencing analysis of large sample size in Chinese populations. Here, we report the primary results of the Chinese Academy of Sciences Precision Medicine Initiative (CASPMI) project launched by the Chinese Academy of Sciences, including the de novo assembly of a northern Han reference genome (NH1.0) and whole genome analyses of 597 healthy people coming from most areas in China. Given the two existing reference genomes for Han Chinese (YH and HX1) were both from the south, we constructed NH1.0, a new reference genome from a northern individual, by combining the sequencing strategies of PacBio, 10× Genomics, and Bionano mapping. Using this integrated approach, we obtained an N50 scaffold size of 46.63 Mb for the NH1.0 genome and performed a comparative genome analysis of NH1.0 with YH and HX1. In order to generate a genomic variation map of Chinese populations, we performed the whole-genome sequencing of 597 participants and identified 24.85 million (M) single nucleotide variants (SNVs), 3.85 M small indels, and 106,382 structural variations. In the association analysis with collected phenotypes, we found that the T allele of rs1549293 in KAT8 significantly correlated with the waist circumference in northern Han males. Moreover, significant genetic diversity in MTHFR, TCN2, FADS1, and FADS2, which associate with circulating folate, vitamin B12, or lipid metabolism, was observed between northerners and southerners. Especially, for the homocysteine-increasing allele of rs1801133 (MTHFR 677T), we hypothesize that there exists a "comfort" zone for a high frequency of 677T between latitudes of 35-45 degree North. Taken together, our results provide a high-quality northern Han reference genome and novel population-specific data sets of genetic variants for use in the personalized and precision medicine.

Subtle regulation of cotton resistance to Verticillium wilt mediated by MAPKK family members.

Verticillium wilt caused by soil-borne fungus of Verticillium dahliae Kleb. is one of the most devastating diseases of cotton. Since the hierarchically organized mitogen-activated protein kinase (MAPK) cascade plays pivotal roles in signaling plant defense against pathogen attack, and the key nodes of MAPKKs (MKKs) may serve as for the convergence and divergence of signals in MAPK cascades, the possible relations between MAPK signaling and cotton Verticillium resistance were examined in this study. A total of 24 MKK genes were identified in the Gossypium hirsutum L. genome and then classified based on phylogenetic analysis. Then the regulation roles of all types of cotton MKKs in activation of cotton disease resistance were tested with the virus-induced gene silencing (VIGS) method. The results showed that three types of MKKs (GhMKK4, GhMKK6 and GhMKK9) positively regulate, while GhMKK10 negatively regulate the cotton resistance to Verticillium wilt. Further, more subtle regulation of cotton resistance mediated by MKK genes were revealed. In GhMKK9, only Gh_A12G2448 and Gh_D12G2574 displayed positive regulation of cotton resistance; whereas only Gh_A12G1883 and Gh_D12G2062 displayed negative regulation of cotton resistance in GhMKK10. All these results show that MKK members in MAPK signal cascades play dual roles in subtly regulating of cotton resistance to Verticillium wilt.

Study of Cotton Leaf Senescence Induced by Alternaria alternata Infection.

Premature leaf senescence in cotton, which often happens during the mid to late growth period, has been occurring with an increasing frequency in many cotton-growing areas and causing serious reduction in yield and quality. One of the key factors causing cotton leaf senescence is the infection of Alternaria leaf spot pathogens (Alternaria species), which often happens when cotton plants encounter adverse environmental conditions, such as chilling stress and physiological impairment. Stressed cotton leaves are apt to be infected by Alternaria leaf spot pathogens (Alternaria alternata) because of the reduction in disease resistance, leading to the initiation of leaf senescence. Here we describe the induction of cotton leaf senescence by Alternaria alternata infection, including the evaluation of the disease index and measure of physiological impairment associated with cotton leaf senescence and analysis of possible molecular mechanism using microarray.