[Determination of echinacoside in congrong spirit by reversed-phase high performance liquid chromatography].

A reversed-phase high performance liquid chromatographic method, which is relatively simple, rapid and effective for echinacoside determination in Congrong spirit, has been developed. With this method, the peak of echinacoside could be separated completely from other interfering components in the spirit. The chromatographic conditions used were a reversed-phase C18 column at 25 degrees C with detection wavelength at 330 nm and CH3CN-1% HAc (14:86 in volume ratio) as mobile phase. A good linear relationship has been obtained between 0.52 microgram to 2.60 micrograms of echinacoside. The recovery was 99.7%.