Enhancement of periodontal tissue regeneration by conditioned media from gingiva-derived or periodontal ligament-derived mesenchymal stem cells: a comparative study in rats.

BACKGROUND: Evidence has demonstrated conditioned medium (CM) from periodontal ligament stem cells (PDLSCs) improved periodontal regeneration. Gingival mesenchymal stem cells (GMSCs) have been considered an alternative strategy for regenerative medicine. To determine whether GMSC-CM could promote periodontal wound healing, we compared the effects of GMSC-CM and PDLSC-CM on periodontal regeneration and the underlying mechanisms in rat periodontal defects. METHODS: Cell-free CMs were collected from PDLSCs, GMSCs, and gingival fibroblasts (GFs) using ultracentrifugation (100-fold concentration). Periodontal defects were created on the buccal side of the first molar in the left mandible of 90 rats by a surgical method. Collagen membranes loaded with concentrated CMs (α-MEM, GF-CM, GMSC-CM, PDLSC-CM) were transplanted into periodontal defects. After 1, 2, and 4 weeks, the animals were sacrificed and specimens including the first molar and the surrounding tissues were separated and decalcified. Hematoxylin-eosin and Masson's trichrome staining were performed to evaluate periodontal regeneration. Immunohistochemical staining for tumor necrosis factor (TNF)-α, interleukin (IL)-1ß, and IL-10 was conducted to analyze inflammation. Immunohistochemistry of BSP-II and Runx2 was performed to analyze osteoblast differentiation. RESULTS: Histological analysis showed the amount of newly formed periodontal tissue was significantly higher in both the GMSC-CM and PDLSC-CM groups than in the other groups, with no significant difference between these two groups. At 1 and 2 weeks, the expression levels of TNF-α and IL-1ß were significantly lower in the GMSC-CM and PDLSC-CM groups than in the other three groups, while there was no significant difference between these two groups. IL-10 expression was significantly higher in the GMSC-CM group than in the PDLSC-CM group and the other three groups. At 1, 2, and 4 weeks, BSP-II and Runx2 expressions were significantly higher in the GMSC-CM and PDLSC-CM groups than in the other three groups, with no significant difference between the two groups. CONCLUSIONS: Our results demonstrate that GMSC-CM transplantation can significantly promote periodontal regeneration in rats and achieve the same effect as PDLSC-CM. The mechanism of periodontal regeneration may involve the regulation of inflammatory factors and the promotion of osteogenic differentiation of bone progenitor cells in the wound region by CMs from MSCs.

The biological behavior optimization of human periodontal ligament stem cells via preconditioning by the combined application of fibroblast growth factor-2 and A83-01 in in vitro culture expansion.

BACKGROUND: As the optimal source of seed cells in periodontal tissue engineering, periodontal ligament stem cells (PDLSCs) have always been researched to improve cell expansion due to their limited resource and spontaneous differentiation in vitro cultivation. Fibroblast growth factor-2 (FGF-2) has been proven to stimulate bone marrow mesenchymal stem cells (BMMSCs) proliferation and maintain their pluripotency when being added to the culture medium. As a small molecule inhibitor of transforming growth factor-beta receptors (TGF-ßRs), A83-01 can also promote cell proliferation. Therefore, the aim of this study was to verify whether the combined application of FGF-2 and A83-01 could augment cell quantity and quality during in vitro culture. METHODS: PDLSCs were preconditioned with A83-01, FGF-2, or their combination. A cell counting kit-8 (CCK8) assay, cell apoptosis assay, ALP activity assay, Alizarin Red S staining assay, RT-PCR assay, Western blot assay and ELISA were used to determine the sustained effects of different preconditioning strategies on the proliferation, apoptosis, stemness, osteogenic differentiation and paracrine action of PDLSCs. RESULTS: The combined application of FGF-2 and A83-01 significantly augmented cell expansion, reduced cell apoptosis, magnified stemness expression, promoted later osteogenic differentiation and mineralization and increased paracrine action of PDLSCs compared with the control. Moreover, the combination presented significant advantages in enhancing proliferation, stemness expression and paracrine action over FGF-2 alone. CONCLUSIONS: The combined application of A83-01 and FGF-2 may be an improved strategy for PDLSCs biological behavior optimization in culture expansion and advantageous for reinforcing proliferation, stemness expression and cytokine secretion over FGF-2 alone.

[Multiple pregnancy epulides: a case report].

Pregnancy epulis is a tumor-like lesion that results from aggravating gingivitis due to high level of gonadal hormone concentration. Pregnancy epulis is commonly manifested as a single lump. A case of multiple pregnancy epulises, which has been rarely explored, is reported in this paper. The patient received no other treatment but a single supragingival scaling. Results showed no remarkable changes in the lesion. However, all the epulides regressed spontaneously in five months after the parturition.

Tensile strain dependence of the Brillouin gain spectrum in carbon/polyimide coated fibers.

The strain dependence of Brillouin gain spectrum in two novel carbon/polyimide coated fibers (CPCFs) is studied, for what we believe to be the first time, by numerical simulation and experiment. The strain dependence of the Brillouin frequency shift for the two CPCFs maintained linear relation with the elongation up to 4.26% and 4.0%, respectively. The effects of strain on the elastic modulus, the core refractive index, and density are determined, which have been used to simulate the strain dependence of the frequency shift, linewidth, and gain coefficient for the two CPCFs. The simulated and measured results are well matched.

Highly birefringent elliptical-hole photonic crystal fiber with squeezed hexagonal lattice.

We propose a novel polarization-maintaining index-guiding photonic crystal fiber (PCF). It is composed of a solid silica core and a cladding with squeezed-hexagonal-lattice elliptical air holes. Using a full-vector finite-element method, we study the modal birefringence of the fundamental modes in such PCFs. Numerical result shows that very high modal birefringence with a magnitude of the order of 10(-2) around 1550 nm has been obtained. Furthermore, large normal dispersion appears over a wide range of wavelengths in both orthogonal polarizations.

Temperature-insensitive fiber Bragg grating dynamic pressure sensing system.

Temperature-insensitive dynamic pressure measurement using a single fiber Bragg grating (FBG) based on reflection spectrum bandwidth modulation and optical power detection is proposed. A specifically designed double-hole cantilever beam is used to provide a pressure-induced axial strain gradient along the sensing FBG and is also used to modulate the reflection bandwidth of the grating. The bandwidth modulation is immune to spatially uniform temperature effects, and the pressure can be unambiguously determined by measuring the reflected optical power, avoiding the complex wavelength interrogation system. The system acquisition time is up to 85 Hz for dynamic pressure measurement, and the thermal fluctuation is kept less than 1.2% full-scale for a temperature range of -10 degrees C to 80 degrees C.

Modal cutoff properties in germanium-doped photonic crystal fiber.

The germanium-doped photonic crystal fiber (PCF) has some characteristics that differentiate it from pure-silica PCF for a germanium element being doped in the core, such as the intensified nonlinearity, the enhanced photosensitivity, and so on. To pave the way for the application of the Ge-doped PCF successfully, it is necessary to study its properties. We investigated the modal cutoff properties of Ge-doped PCF quantitatively by using the beam propagation method. The numerical results show that the effective refractive indices and the normalized frequency V of Ge-doped PCF not only depend on the normalized pitch delta/lambda but also depend on the normalized hole size d/delta, the modal cutoff boundary for the single mode-multimode of the Ge-doped PCF shift to the low d/delta side in contrast to the pure-silica PCF.

Tunable highly birefringent photonic bandgap fibers.

A novel tunable highly birefringent photonic bandgap fiber (PBGF) is designed theoretically by filling its air holes with high-index material. The transmission band can be continuously tuned by changing the refractive index of the filling material. Accordingly, the tunable modal birefringence and polarization mode dispersion of the PBGFs are investigated by adjusting the refractive index of the filling material. Furthermore, we have also analyzed the effect of surface modes in the photonic bandgap on the characteristics of the tunable PBGFs. The simulation results show the feasibility of constructing birefringence-tunable photonic crystal fibers and related fiber devices in practical applications.

Coupling and decoupling of dual-core photonic bandgap fibers.

Coupling characteristics of dual-core photonic bandgap fibers with triangular photonic crystal cladding are investigated by use of a vector plane-wave expansion method and a vector finite-element method. We demonstrate the eigenmodes and the coupling length for two orthogonal polarizations. A decoupling phenomenon is found at a certain wavelength in this fiber configuration. The decoupling effect is attributed to the effect of surface modes on the eigenmodes. The decoupling wavelength decreases as the ratio of core radius to cladding air-hole pitch increases from 1.05 to 1.15.

Transformation of a transmission mechanism by filling the holes of normal silica-guiding microstructure fibers with nematic liquid crystal.

Transformation of an optical transmission mechanism was achieved when the holes of normal silica-guiding microstructure fiber (MF) were filled with nematic liquid crystal (NLC). Moreover, two photonic bandgaps (PBGs) were obtained by using a plane-wave method to create the pattern. The wavelength dependence of the effective mode area, leakage loss, and group velocity dispersion (GVD) has been theoretically investigated by using a full-vector finite-element method with anisotropic perfectly matched layers. The results reveal that the characteristics of the NLC-filled PBG-MFs are particularly wavelength dependent. This research gives a physical insight into the propagation mechanism in MFs and is crucial for future transmission applications.