Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Nutr Biochem ; 28: 129-39, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26878790

RESUMO

Common beans are rich in phenolic compounds and nondigestible fermentable components, which may help alleviate intestinal diseases. We assessed the gut health priming effect of a 20% cranberry bean flour diet from two bean varieties with differing profiles of phenolic compounds [darkening (DC) and nondarkening (NDC) cranberry beans vs. basal diet control (BD)] on critical aspects of gut health in unchallenged mice, and during dextran sodium sulfate (DSS)-induced colitis (2% DSS wt/vol, 7 days). In unchallenged mice, NDC and DC increased (i) cecal short-chain fatty acids, (ii) colon crypt height, (iii) crypt goblet cell number and mucus content and (iv) Muc1, Klf4, Relmß and Reg3γ gene expression vs. BD, indicative of enhanced microbial activity and gut barrier function. Fecal 16S rRNA sequencing determined that beans reduced abundance of the Lactobacillaceae (Ruminococcus gnavus), Clostridiaceae (Clostridium perfringens), Peptococcaceae, Peptostreptococcaceae, Rikenellaceae and Pophyromonadaceae families, and increased abundance of S24-7 and Prevotellaceae. During colitis, beans reduced (i) disease severity and colonic histological damage, (ii) increased gene expression of barrier function promoting genes (Muc1-3, Relmß, and Reg3γ) and (iii) reduced colonic and circulating inflammatory cytokines (IL-1ß, IL-6, IFNγ and TNFα). Therefore, prior to disease induction, bean supplementation enhanced multiple concurrent gut health promoting parameters that translated into reduced colitis severity. Moreover, both bean diets exerted similar effects, indicating that differing phenolic content did not influence the endpoints assessed. These data demonstrate a proof-of-concept regarding the gut-priming potential of beans in colitis, which could be extended to mitigate the severity of other gut barrier-associated pathologies.


Assuntos
Colite/dietoterapia , Dieta , Inflamação/dietoterapia , Microbiota , Phaseolus , Animais , Fezes/microbiologia , Mediadores da Inflamação/metabolismo , Fator 4 Semelhante a Kruppel , Camundongos , Camundongos Endogâmicos C57BL , Filogenia , RNA Mensageiro/genética
2.
J Nutr Biochem ; 26(7): 752-60, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25841250

RESUMO

Common beans are a rich source of nondigestible fermentable components and phenolic compounds that have anti-inflammatory effects. We assessed the gut-health-promoting potential of kidney beans in healthy mice and their ability to attenuate colonic inflammation following dextran sodium sulphate (DSS) exposure (via drinking water, 2% DSS w/v, 7 days). C57BL/6 mice were fed one of three isocaloric diets: basal diet control (BD), or BD supplemented with 20% cooked white (WK) or dark red kidney (DK) bean flour for 3 weeks. In healthy mice, anti-inflammatory microbial-derived cecal short chain fatty acid (SCFA) levels (acetate, butyrate and propionate), colon crypt height and colonic Mucin 1 (MUC1) and Resistin-like Molecule beta (Relmß) mRNA expression all increased in WK- and DK-fed mice compared to BD, indicative of enhanced microbial activity, gut barrier integrity and antimicrobial defense response. During colitis, both bean diets reduced (a) disease severity, (b) colonic histological damage and (c) increased mRNA expression of antimicrobial and barrier integrity-promoting genes (Toll-like Receptor 4 (TLR4), MUC1-3, Relmß and Trefoil Factor 3 (TFF3)) and reduced proinflammatory mediator expression [interleukin (IL)-1ß, IL-6, interferon (IFN)γ, tumor necrosis factor (TNF)α and monocyte chemoattractant protein-1], which correlated with reduced colon tissue protein levels. Further, bean diets exerted a systemic anti-inflammatory effect during colitis by reducing serum levels of IL-17A, IFNγ, TNFα, IL-1ß and IL-6. In conclusion, both WK and DK bean-supplemented diets enhanced microbial-derived SCFA metabolite production, gut barrier integrity and the microbial defensive response in the healthy colon, which supported an anti-inflammatory phenotype during colitis. Collectively, these data demonstrate a beneficial colon-function priming effect of bean consumption that mitigates colitis severity.


Assuntos
Colite/prevenção & controle , Modelos Animais de Doenças , Alimento Funcional , Mucosa Intestinal/imunologia , Phaseolus/química , Pigmentos Biológicos/biossíntese , Sementes/química , Animais , Colite/imunologia , Colite/metabolismo , Colite/patologia , Colo/imunologia , Colo/metabolismo , Colo/microbiologia , Colo/patologia , Citocinas/antagonistas & inibidores , Citocinas/sangue , Citocinas/metabolismo , Sulfato de Dextrana , Ácidos Graxos Voláteis/análise , Ácidos Graxos Voláteis/metabolismo , Fermentação , Alimento Funcional/análise , Conteúdo Gastrointestinal/química , Conteúdo Gastrointestinal/microbiologia , Microbioma Gastrointestinal/imunologia , Regulação da Expressão Gênica , Hormônios Ectópicos/genética , Hormônios Ectópicos/metabolismo , Imunidade nas Mucosas , Peptídeos e Proteínas de Sinalização Intercelular , Mucosa Intestinal/metabolismo , Mucosa Intestinal/microbiologia , Mucosa Intestinal/patologia , Masculino , Camundongos Endogâmicos C57BL , Mucina-1/genética , Mucina-1/metabolismo , Phaseolus/metabolismo , Distribuição Aleatória , Sementes/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...