[HPLC fingerprint analysis of acetoacetate extraction of Polygonum orientale].

OBJECTIVE: To establish the HPLC fingerprint analysis method for the quality control of acetoacetate extraction of Polygonum orientale. METHODS: Agilent C18 (4.6 mm x 250 mm, 5 microm); DAD detector; Mobile phase: methyl alcohol-water gradient elution at a flow rate of 1.0 mL/min, and at a column temperature of 30 degrees C. RESULTS: 26 peaks were selected as the common fingerprint peaks. The relative standard deviations for relative retention values were less than 3% in the precision and repeated test. CONCLUSION: The repetition, stability and precision of the method is good and it can be used to control the quality of Polygonum orientale.