Apolipoprotein E mimetic peptide COG1410 combats pandrug-resistant Acinetobacter baumannii.

The emergence of pandrug-resistant bacteria breaks through the last line of defense and raises fear among people of incurable infections. In the post-antibiotic era, the pharmaceutical field turns to seek non-conventional anti-infective agents. Antimicrobial peptides are considered a prospective solution to the crisis of antimicrobial resistance. In this study, we evaluated the antimicrobial efficiency of an ApoE mimetic peptide, COG1410, which has been confirmed to exhibit strong neural protective activity and immunomodulatory function. COG1410 showed potent antimicrobial activity against pandrug-resistant Acinetobacter baumannii, even eliminating large inocula (108 CFU/ml) within 30 min. LC99.9 in PBS and 50% pooled human plasma was 2 µg/ml (1.4 µM) and 8 µg/ml (5.6 µM), respectively. Moreover, COG1410 exhibited biofilm inhibition and eradication activity, excellent stability in human plasma, and a low propensity to induce resistance. Although COG1410 easily entered bacterial cytoplasm and bound to DNA nonspecifically, the major mechanism of COG1410 killing was to disrupt the integrity of cell membrane and lead to leakage of cytoplasmic contents, without causing obvious pores on the cell surface or cell lysis. Additionally, transcriptome analysis showed that treatment with COG1410-enriched genes involved a series of oxidation-reduction processes. DCFH-DA probe detected an increased ROS level in the presence of COG1410, indicating ROS was another hit of this AMP. Furthermore, the action of COG1410 did not depend on the electronic interaction with the LPS layer, in contrast to polymyxin B. The strong synergistic interaction between COG1410 and polymyxin B dramatically reduced the working concentration of COG1410, expanding the safety window of the application. C. elegans infection model showed that combined therapy of COG1410 and polymyxin B was capable of significantly rescuing the infected nematodes. Taken together, our study demonstrates that COG1410 is a promising drug candidate in the battle against pandrug-resistant A. baumannii.

Internet use and land transfer in: empirical evidence from China's rural panel data.

In the context of the Internet power strategy, "Internet + agriculture" has gradually become a key force in realizing agricultural modernization. Based on the data of the China Labor-force Dynamics Survey 2012, 2014, and 2016, this paper uses conditional mixed process (CMP) to solve the endogeneity problem and tests the impact and mechanism of Internet use on farmers' land transfer in. The results showed that (1) from 2012 to 2016, the proportion of farmers using the Internet increased in turn, while the proportion of farmers transferring in land decreased in turn. (2) Farmers' Internet use is significantly negatively correlated with land transfer in. In 2012, 2014, and 2016, each unit increase in the proportion of farmers' Internet use reduces the probability of land transfer in by 2.6%, 10.7%, and 20.8%, respectively. (3) The analysis of mediating effects shows that the Internet can promote land transfer in by expanding social networks and inhibit land transfer in by improving the stability of non-agricultural employment, and both are partial mediating effects. From the micro-perspective of farmers' Internet use, this study can strengthen our understanding of Internet use and land transfer behavior decision-making and then provide a reference for rural digitalization construction and land transfer-related policy making.

Peer Effects on Farmers' Purchases of Policy-Based Planting Farming Agricultural Insurance: Evidence from Sichuan Province, China.

The tendency to conform with peers, and learning by imitation, have become new influencing factors that affect farmers' purchases of policy-based planting agricultural insurance. Based on the survey data of 540 farmers in Sichuan Province in 2021, this study empirically analyzed the impact of peer effects on farmers' purchases of policy-based planting agricultural insurance and its mechanism. The results show that: (1) Regardless of whether farmers' relatives and friends visit during the New Year period, the purchase of policy-based planting agricultural insurance by relatives and friends will positively and significantly affect the purchasing behavior of the farmers. (2) The impact of the peer effect on the behavior of farmers purchasing policy-based planting agricultural insurance is related to the relationship between the strengths and weaknesses. (3) The results of the mechanism analysis show that, through the mediating variables of social network and trust, the influence of the peer effect is weakened. (4) Heterogeneity analysis shows that farmers having a larger land scale and higher educational background are more influenced by the same peer effect. The results of the study emphasize the importance of the peer effect on the behavior of farmers purchasing policy-based planting agricultural insurance, and can provide a decision-making reference for the formulation of related policies.

Does Trust Help to Improve Residents' Perceptions of the Efficacy of Disaster Preparedness? Evidence from Wenchuan and Lushan Earthquakes in Sichuan Province, China.

Disaster preparation can reduce the impact of an earthquake on residents. Residents are more likely to undertake disaster preparedness if they perceive it to be effective. However, few studies have analyzed the influence of trust on this perception. This study surveyed 327 households in areas stricken by the Wenchuan and Lushan earthquakes to explore these issues. Trust was divided into government trust, emotional trust, and social trust, while the efficacy of disaster preparedness was divided into self-efficacy and response efficacy. A partial least squares structural equation model was used to explore the influence of trust on perceptions of the efficacy of disaster preparedness. The results show that: (1) government trust can directly increase perceived efficacy and indirectly increase self-efficacy via emotional trust; (2) emotional trust can directly increase self-efficacy; (3) social trust can directly reduce self-efficacy while indirectly increasing it by increasing emotional trust. This study deepens our understanding of the relationship between trust and perceptions of the efficacy of disaster preparedness. This study can provide inspiration to improve risk communication and construct systems of community-based disaster-prevention.