RESUMO
Enterovirus 71 (EV71) is the major causative agent of hand, foot, and mouth disease and induces fatal neurological complications. In recent years, this virus has become a major threat to public health in the Asia-Pacific region, while no effective antiviral therapies and vaccines are currently available. In this study, we constructed and characterized for the first time an infectious full-length EV71 cDNA clone derived from the SHZH98 strain, which was the first subgenotype C4 strain isolated in China. Our data demonstrate that the rescued EV71 viruses exhibited growth kinetics in vitro and morphologies similar to those of the BrCr-TR strain and reached a maximum titer of 10(7.5) TCID50/ml. Although the rescued viruses were able to infect suckling mice, no typical symptoms of EV71 infection were observed for up to 18 days post-inoculation. Taken together our research provides an important tool to study the epidemic strains of EV71 in the Asia-Pacific region and promote the development of vaccines.
Assuntos
DNA Complementar/genética , Enterovirus Humano A/genética , Enterovirus Humano A/fisiologia , RNA Viral/genética , Animais , China , DNA Complementar/química , Modelos Animais de Doenças , Enterovirus Humano A/isolamento & purificação , Genótipo , Doença de Mão, Pé e Boca/patologia , Doença de Mão, Pé e Boca/virologia , Camundongos , Dados de Sequência Molecular , Análise de Sequência de DNA , Carga Viral , Cultura de Vírus , Replicação ViralRESUMO
We detected Bartonella quintana in 48.6% of captive rhesus macaques from an animal facility in Beijing, China. Prevalence of infection increased over the period of observation. Our findings suggest that macaques may serve as reservoir hosts for B. quintana and that Pedicinus obtusus lice might act as efficient vectors.
