Each Personality Performs Its Own Function: Boldness and Exploration Lead to Differences in the Territoriality of Swimming Crabs (Portunus trituberculatus).

The boldness and exploration of animals are closely related to their territoriality, with relevant studies having important applications in wildlife conservation. The present study establishes a behavior observation system measuring the boldness and exploration of swimming crabs (Portunus trituberculatus) to clarify the relationship between boldness, exploration, and territoriality, as well as to provide a behavioral basis for the construction of marine ranching. The behavioral tests of crabs in a safe environment (predator absence), a dangerous environment (predator presence), and habitat selection (complex and simple habitat) are analyzed. A territorial behavior score is calculated as an evaluation index of territoriality. The correlation between the swimming crabs' boldness, exploration, and territoriality is analyzed. The results show that there is no boldness-exploratory behavioral syndrome. In predator absence or presence environments, boldness is dominant in territorial behavior and positively correlates with territoriality. Exploration plays a vital role in habitat selection tests but has no significant correlation with territoriality. The experimental results preliminarily show that boldness and exploration jointly develop the difference in the space utilization ability of crabs with different personalities, improving the adaptability of swimming crabs in different conditions. The results of this study supplement the behavior rules of the dominant species of typical fishery resources in marine ranches, providing a basis for achieving animal behavior management function in marine ranches.

High temperatures enhance the strength of multiple predator effects in a typical crab-clam system.

Although marine heatwaves pose urgent threats to marine life, our understanding of how these events influence interactions between key species in marine ecosystems is still inadequate. Herein, we examined the behavioral mechanisms by which heat regulates multiple predator effects in different foraging systems that include Asian paddle crabs (Charybdis japonica) and swimming crabs (Portunus trituberculatus) by quantifying their predation and competition at two temperatures. Our results show that non-independent multiple predator effects occurred in the conspecific treatment of Asian paddle crabs and in the interspecific treatment, whereas independent multiple predator effects occurred in the conspecific treatment of swimming crabs. Asymmetrical behavior responses of these crabs to competition and heat triggered divergences in multiple predator effects. High temperatures increased the strength of multiple predator effects but did not alter their types. The reason is that heat negatively impacts predation by enhancing aggressive interactions, outweighing its direct positive effects on predation.

Serotonin and dopamine regulate the aggressiveness of swimming crabs (Portunus trituberculatus) in different ways.

Bioamines act as a pivotal part in the regulation of aggressive behavior in animals as a type of neuroendocrine, but the patterns of how they regulate aggressiveness in crustaceans are still unclear due to species-specific responses. To determine the effects of serotonin (5-HT) and dopamine (DA) on the aggressiveness of swimming crabs (Portunus trituberculatus), we quantified their behavioral and physiological characteristics. The results showed that an injection of 5-HT at 0.5 mmol L-1 and 5 mmol L-1 could significantly enhance the aggressiveness of swimming crabs, as well as an injection of DA at 5 mmol L-1. The regulation of 5-HT and DA on aggressiveness is dose-dependent, and these two bioamines have different concentration thresholds that can trigger aggressiveness changes. 5-HT could up-regulate the 5-HTR1 gene expression and increase lactate content at the thoracic ganglion as the aggressiveness enhances, suggesting that 5-HT may activate related receptors and neuronal excitability to regulate aggressiveness. As a result of DA injection at 5 mmol L-1, lactate content in the chela muscle and hemolymph increased, glucose content in the hemolymph increased, and the CHH gene was significantly up-regulated. Pyruvate kinase and hexokinase enzyme activities in the hemolymph increased, which accelerated the glycolysis process. These results demonstrate that DA regulates the lactate cycle, which provides substantial short-term energy for aggressive behavior. Both 5-HT and DA can mediate aggressive behavior in the crab by activating calcium regulation in muscle tissue. We conclude that the enhancement of aggressiveness is a process of energy consumption, in which 5-HT acts on the central nervous system to induce aggressive behavior, and DA affects muscle and hepatopancreas tissue to provide a large amount of energy. This study expands upon the knowledge of regulatory mechanisms of aggressiveness in crustaceans and offers a theoretical foundation for enhancing crab culture management.

The Effect of Intruder Density on Territoriality and Dominance in Male Swimming Crab (Portunus trituberculatus).

Territorial behavior of animals is affected by numerous factors, one being the number of intruders. The swimming crab (Portunus trituberculatus), an important commercial and ecological species on the continental shelf of Asia, usually needs to defend its territory from intrusion by other crabs, especially in habitats with high densities of conspecifics. To clarify the underlying patterns of how P. trituberculatus protects its territory, we assessed the territorial behavior of occupant crabs (territory holders) when presented with different numbers of intruders using an indoor observation system. We calculated the territory size of the occupants and quantified their behavioral responses to intruders. With an increased number of intruders, the territory size the occupants owned significantly decreased, and their behavior adjusted accordingly. Besides, the territorial behavior score, reflecting the territoriality of crab, decreased significantly. Furthermore, in a high density group that had seven intruders, the occupants showed a higher dominance hierarchy than the intruders, indicating the ascendancy of occupants in territorial competition with intruders. These results revealed that as the number of intruders increased, the territory size of P. trituberculatus shrunk because the fight for territory became more intense.