Influenza virus H1N1 induced apoptosis of mouse astrocytes and the effect on protein expression.

OBJECTIVE: To investigate the effects of influenza A virus H1N1 infection on the proliferation and apoptosis of mouse astrocytes cells and its protein expression. METHODS: After mouse astrocytes was infected with purified influenza A virus H1N1 in vitro, viral integration and replication status of the cells were detected by RT-PCR assay, cell proliferation and apoptosis was determined by MTT method and flow cytometry, respectively. Associated protein expression was detected by Western blotting. RESULTS: Agarose gel electrophoresis showed H1N1 virus can infect astrocytes and can be copied. MTT staining showed H1N1 virus infection can inhibit the proliferation of mouse astrocytes, which makes cell viability decreased significantly. Flow cytometry showed that the proportion of Annein V staining positive vascular endothelial cells in the influenza A virus group was significantly higher than that in the control group. Western blot analysis showed after 24 h and 32 h of infection, there were cells caspase-3 protein and the expression of its active form (lysed caspase-3 protein) increased. The proportion of Bax/Bcl-2 also increased. CONCLUSIONS: Influenza A virus can infect human vascular endothelial cells and proliferation and it can induce apoptosis of endothelial cells.

Bis{µ-2-[1-(2-Pyridylmethyl-imino)eth-yl]phenolato}bis-[azido-copper(II)].

The title compound, [Cu(2)(C(14)H(13)N(2)O)(2)(N(3))(2)], was synthesized by the reaction of Cu(NO(3))(2)·3H(2)O with the Schiff base 2-[1-(2-pyridylmethyl-imino)eth-yl]phenol (HL) in methanol-water solution, adding NaN(3) as the bridging ligand. The asymmetric unit contains one half-mol-ecule, the other half being generated by the inversion center. Each Cu(II) atom shows a slightly distorted trigonal-pyramidal geometry formed by two N atoms and one O atom from one Schiff base ligand, by another O atom of a second Schiff base ligand and by an azide N atom. The crystal structure is stabilized by intermolecular C-H⋯N hydrogen bonds.

µ(3)-Oxido-hexa-µ(2)-pivalato-tris[(methanol-κO)cobalt(III)] chloride.

The crystal structure of the title compound, [Co(3)(C(5)H(9)O(2))(6)O(CH(4)O)(3)]Cl, consists of trinuclear Co(III) complex cations and chloride anions. The Co(III) cation has site symmetry m, and is coordinated by four oxygen atoms from four bridging pivalate groups, one central O anion and a methanol oxygen atom, forming a distorted octa-hedral geometry. The coordinated methanol mol-ecule is located on a crystallographic special position, the C and O atoms being located on the mirror plane. The central O anion lies in the crystallographic position, and acts as a µ(3)-O bridge, linking three equivalent Co(III) cations and generating the oxo-centered trinuclear Co(III) complex. The chloride anion, which acts as the counter-ion, is located on crystallographic position. O-H⋯Cl hydrogen bonding between the Cl anion and hydroxyl group of the coordinated methanol mol-ecule links the mol-ecules into a supra-molecular network.

4-(6-Fluoro-1,2-benzisoxazol-3-yl)-1-[2-(2-methyl-4-oxo-6,7,8,9-tetra-hydro-4H-pyrido[1,2-a]pyrimidin-3-yl)eth-yl]piperidinium nitrate.

In the risperidone cation of the title compound, C(23)H(28)FN(4)O(2) (+)·NO(3) (-), the piperidine ring adopts a chair conformation and the tetra-hydro-pyridine ring is disordered over two orientations in a 0.620 (11):0.380 (11) ratio. N-H⋯O, C-H⋯O and C-H⋯F hydrogen bonds are present in the crystal structure.

Di-µ-chlorido-bis-[aqua-(2,2'-bipyridine-κN,N')chloridocobalt(II)].

The title complex, [Co(2)Cl(4)(C(10)H(8)N(2))(2)(H(2)O)(2)], is composed of two Co(II) atoms, each hexa-coordinated by three Cl atoms, one 2,2'-bipyridine (bpy) ligand and one water mol-ecule in a distorted octa-hedral geometry. Neighboring Co(II) atoms are linked together by two Cl bridges, forming a dinuclear Co(II) complex with inversion symmetry. There are inter-molecular O-H⋯Cl hydrogen bonds and inter-molecular π-π stacking inter-actions between adjacent bpy ligands [centroid-centroid distance = 3.617 (2) Å] in the structure.

Aqua-(1,10-phenanthroline-κN,N')bis-(trimethyl-acetato)-κO,O';κO-cobalt(II).

In the title compound, [Co(C(5)H(9)O(2))(2)(C(12)H(8)N(2))(H(2)O)], the Co(II) atom is coordinated in a distorted octahedral environment by three carboxyl O atoms of two trimethyl-acetate ligands, one aqua O atom and two N atoms from 1,10-phen-anthroline. The crystal structure is stabilized by O-H⋯O hydrogen bonds and π-π stacking inter-actions [inter-planar distance between inter-digitating 1,10-phenanthroline ligands = 3.378 (2) Å].

Aqua-bis(2-methyl-4-oxopyrido[1,2-a]pyrimidin-9-olato)zinc(II) monohydrate.

The crystal structure of the title compound, [Zn(C(9)H(7)N(2)O(2))(2)(H(2)O)]·H(2)O, involves discrete mononuclear complex mol-ecules. The special positions on the rotation twofold axis are occupied by Zn(II) and O atoms of the coordinated and uncoordinated water mol-ecules. The coordination around the Zn(II) atom can be described as transitional from trigonal-bipyramidal to square-pyramidal. The two chelating 2-methyl-4-oxopyrido[1,2-a]pyrimidin-9-olate ligands and the coordin-ated water mol-ecule form the Zn coordination. O-H⋯O hydrogen bonds between the coordinated water mol-ecule and the ligand and between the uncoordinated water mol-ecule and the ligand dominate the crystal packing.