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1.
RSC Chem Biol ; 5(7): 691-702, 2024 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-38966676

RESUMO

Covalent protease inhibitors serve as valuable tools for modulating protease activity and are essential for investigating the functions of protease targets. These inhibitors typically consist of a recognition motif and a covalently reactive electrophile. Substrate peptides, featuring residues capable of fitting into the substrate pockets of proteases, undergo chemical modification at the carbonyl carbon of the P1 residue with an electrophile and have been widely applied in the development of covalent inhibitors. In this study, we utilized a DNA-encoded peptide library to replicate peptide binder sequences and introduced a vinyl sulfone warhead at the C-termini to construct the DNA-encoded peptide covalent inhibitor library (DEPCIL) for targeting cysteine proteases. Screening results toward 3CL protease demonstrated the efficacy of this library, not only in identifying protease inhibitors, but also in discovering amino acids that can conform to aligned protease pockets. The identified peptide sequences provide valuable insight into the amino acid preferences within substrate binding pockets, and our novel technology is indicative of the potential for similar strategies to discover covalent inhibitors and profile binding preferences of other proteases.

2.
J Nanobiotechnology ; 21(1): 492, 2023 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-38115145

RESUMO

Effective diagnosis and therapy for bacterial infections, especially those caused by multidrug-resistant (MDR) species, greatly challenge current antimicrobial stewardship. Monocytes, which can chemotactically migrate from the blood to infection site and elicit a robust infection infiltration, provide a golden opportunity for bacterial theranostics. Here, a nano-Trojan Horse was facilely engineered using mannose-functionalized manganese-eumelanin coordination nanoparticles (denoted as MP-MENP) for precise two-step localization and potent photothermal-immunotherapy of MDR bacterial infection. Taking advantage of the selective recognition between mannose and inflammation-associated monocytes, the MP-MENP could be passively piggybacked to infection site by circulating monocytes, and also actively target infiltrated monocytes that are already accumulated in infection microenvironment. Such dual-pronged targeting enabled an efficient imaging diagnosis of bacterial infection. Upon laser irradiation, the MP-MENP robustly produced local hyperemia to ablate bacteria, both extracellularly and intracellularly. Further combined with photothermal therapy-induced immunogenic cell death and MP-MENP-mediated macrophage reprogramming, the immunosuppressive infection microenvironment was significantly relieved, allowing an enhanced antibacterial immunity. Collectively, the proposed nanotheranostic Trojan Horse, which integrates dual-pronged targeting, precise imaging diagnosis, and high-performance photothermal immunotherapy, promises a new way for complete eradication of MDR bacterial infection.


Assuntos
Infecções Bacterianas , Nanopartículas , Humanos , Nanomedicina Teranóstica , Manose , Farmacorresistência Bacteriana Múltipla , Infecções Bacterianas/tratamento farmacológico , Nanopartículas/uso terapêutico , Bactérias , Imunoterapia/métodos
3.
Nephrol Ther ; 18(6): 549-556, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-36123282

RESUMO

The present study aimed to investigate the efficacy and safety of tacrolimus for treating incipient minimal change disease in adults. The clinical data of 52 adult patients with minimal change disease of nephrotic syndrome diagnosed by renal biopsy in the First affiliated hospital of Zhengzhou University between August 2013 and August 2015 were retrospectively analyzed. According to the treatment plan, the patients were divided into a tacrolimus group and a glucocorticoid group. The efficacy and safety of tacrolimus in the treatment of minimal change disease in adult patients was analyzed and compared with that of glucocorticoids. The results revealed that the baseline characteristics of the two groups were similar (P>0.05). At 24 weeks, there was a significant difference in serum albumin between the two groups (P<0.01). The serum albumin levels of tacrolimus group was higher compared with the glucocorticoid group. In addition, the complete remission rates in the tacrolimus and glucocorticoid groups were 93.75 and 77.8%, respectively (P=0.095), and the mean complete remission time was 6.33±4.21 and 5.14±2.45 weeks, respectively (P=0.175). The relapse rate was 12.5 and 22.2% in the tacrolimus and glucocorticoid groups, respectively (P=0.368). During the follow-up, in tacrolimus group, the incidence of new onset diabetes or impaired glucose tolerance, osteoporosis, infection, abnormal liver function, Cushing's syndrome, acne and gastrointestinal symptoms were significantly less than those of glucocorticoids (P<0.05). In conclusion, tacrolimus treatment after short-time intravenous methylprednisolone is an effective treatment option with fewer adverse effects in adult onset minimal change disease.


Assuntos
Nefrose Lipoide , Síndrome Nefrótica , Adulto , Humanos , Tacrolimo/efeitos adversos , Nefrose Lipoide/tratamento farmacológico , Metilprednisolona/efeitos adversos , Estudos Retrospectivos , Glucocorticoides/uso terapêutico , Imunossupressores/uso terapêutico , Síndrome Nefrótica/etiologia , Resultado do Tratamento , Albumina Sérica/análise
4.
RSC Chem Biol ; 3(1): 18-31, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-35128405

RESUMO

In contrast to the major families of small molecules and antibodies, cyclic peptides, as a family of synthesizable macromolecules, have distinct biochemical and therapeutic properties for pharmaceutical applications. Cyclic peptide-based drugs have increasingly been developed in the past two decades, confirming the common perception that cyclic peptides have high binding affinities and low metabolic toxicity as antibodies, good stability and ease of manufacture as small molecules. Natural peptides were the major source of cyclic peptide drugs in the last century, and cyclic peptides derived from novel screening and cyclization strategies are the new source. In this review, we will discuss and summarize 18 cyclic peptides approved for clinical use in the past two decades to provide a better understanding of cyclic peptide development and to inspire new perspectives. The purpose of the present review is to promote efforts to resolve the challenges in the development of cyclic peptide drugs that are more effective.

5.
J Food Biochem ; 45(11): e13952, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34636046

RESUMO

Gestational diabetes mellitus (GDM) is a dangerous complication of pregnancy which is induced via dysfunction in glucose metabolism during pregnancy. Sinomenine (SM) has already proved an antidiabetic effect against streptozotocin (STZ)-induced diabetes mellitus (DM) in rats. In this protocol, we examined the protective effect of SM against STZ-induced GDM in rats. Wistar rats were divided into three groups and STZ (40 mg/kg) was used to induce GDM. At the end of the experimental protocol, bodyweight, pub weight, and survival rate were estimated. Blood glucose level (BGL), fasting insulin (FINS), free fatty acid (FFA), Hemoglobin A1C (HbA1c), and C-peptide were measured. Lipid, antioxidant, inflammatory cytokines, and inflammatory mediators were also determined. RT-PCR was used for estimation of the role of TLR4/MyD88/NF-κB signaling pathway. SM treatment significantly (p < .001) reduced BGL, hepatic glycogen, and improved the levels of FINS, C-peptide, FFA, and HbA1c. SM significantly (p < .001) suppressed the levels of total cholesterol (TC), low-density lipoprotein (LDL), triglycerides (TG), coronary artery index (CAI), very low-density lipoprotein (VLDL), atherogenic index (AI), and boosted high-density lipoprotein (HDL) levels. SM significantly (p < .001) decreased the lipid peroxidation (LPO) level and enhanced glutathione peroxidase (GPx), total antioxidant capacity (TAC), glutathione S-transferase (GST), superoxide dismutase (SOD), respectively. It reduced the levels of inflammatory cytokines including interleukin-1ß (IL-1ß), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and inflammatory mediators viz., nuclear kappa B factors (NF-κB). SM significantly (p < .001) reduced the mRNA expression of Myd88, NLRP3, TLR4, and NF-κB, which were boosted in the GDM group rats. These findings suggest that SM could be a probable drug to be used for treating GDM via inhibition of the TLR4 signaling pathway. PRACTICAL APPLICATIONS: It is well known that gestational diabetes mellitus (GDM) is a dangerous health problem during the pregnancy. SM reduced the glucose level; boosted the level of fasting insulin (FINS) and bodyweight. SM significantly improved the number of pubs and their survival rates. SM suppressed oxidative stress and inflammation via activation of TLR4/MyD88/NF-κB signaling pathway. According to our research, SM can be used as a preventive drug in the treatment of GDM during pregnancy.


Assuntos
Diabetes Gestacional , Animais , Diabetes Gestacional/tratamento farmacológico , Feminino , Inflamação/tratamento farmacológico , Morfinanos , Fator 88 de Diferenciação Mieloide/metabolismo , NF-kappa B/metabolismo , Estresse Oxidativo , Gravidez , Ratos , Ratos Wistar , Transdução de Sinais , Receptor 4 Toll-Like/metabolismo
6.
Int J Clin Exp Pathol ; 13(5): 869-879, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32509057

RESUMO

PURPOSE: Ovarian cancer is a common and fatal cancer in women. The long non-coding RNA (lncRNA) MEG3 was reported to affect the cellular processes of ovarian cancer, but the mechanisms remain unclear. Here, we aimed to explore the potential regulatory mechanism of MEG3 in ovarian cancer. MATERIALS AND METHODS: A reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was conducted to analyze the expression levels of MEG3 and miR-205-5p in tissues and cell lines. An MTT assay was utilized to determine the cell viability of ovarian cancer SKOV-3 and OVCAR-8 cells. A flow cytometry analysis was employed to disclose the ovarian cancer cell apoptosis. The migration and invasion of SKOV-3 and OVCAR-8 cells were examined using a Transwell assay. A bioinformatics analysis indicated miR-205-5p as a direct target of MEG3, and a luciferase reporter assay was conducted to validate the interaction between MEG3 and miR-205-5p. RESULTS: MEG3 was significantly down-regulated, while miR-205-5p was up-regulated in ovarian cancer tissues and cell lines. The overexpression of MEG3 and the knockdown of miR-205-5p inhibited cell viability, migration and invasion but promoted the apoptosis rate in ovarian cancer cells. MiR-205-5p was identified as a downstream gene of MEG3 and is negatively regulated by MEG3. The introduction of miR-205-5p reversed the up-regulation of MEG3-mediated suppression effects on cell viability, migration and invasion and increased cell apoptosis in ovarian cancer cells. CONCLUSION: The overexpression of lncRNA MEG3 inhibits cell proliferation and cell invasion and promotes apoptosis in ovarian cancer by sponging miR-205-5p.

7.
Sensors (Basel) ; 19(19)2019 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-31590299

RESUMO

Coral reefs are important as they can help to maintain ecological balance, biological resources, and species diversity on earth. However, they are globally threatened by human activities and climate change. As live coral cover (LCC) is regarded as an important measure of the health of coral reefs, analysis on LCC change associated with environmental parameters, such as chlorophyll-a concentration (Chl-a), sea surface temperature (SST), and photosynthetically active radiation (PAR), is of great value. Research on this front would help us comprehend the changes in coral reefs induced by human activities and global changes. Instead of using spasmodically in-field-measured environmental parameters, in this study, we chose to combine the successive Chl-a, SST, and PAR products of the Moderate Resolution Imaging Spectroradiometer (MODIS) with historic LCC records to establish an empirical relationship using nonlinear optimization. Thereafter, the established relationship was further used to discuss some possible developments of LCCs. According to the experiments, we concluded that the degradation of the LCC around Weizhou Island may be mainly caused by human-activity-caused eutrophication. Besides, we also showed that even if the Chl-a and the PAR can keep constant with current average levels, the corals around Weizhou Island may still be in a risk of disappearing between 2120-2140 as the SST continues to rise.

8.
Immunopharmacol Immunotoxicol ; 33(3): 498-503, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21226554

RESUMO

INTRODUCTION: High interferon-γ (IFN-γ) expression in tumors has been reported to be a favorable prognostic marker. Continuous exposure of ovarian cancer cells to IFN-γ was previously shown to result in significant growth inhibition and apoptosis. Our goal in this study was to evaluate the effect of plasmid-mediated stable IFN-γ expression on the SKOV-3 human ovarian carcinoma cell line. METHODS: SKOV-3 cells were stably transfected with the pEGFP-IFN-γ plasmid. IFN-γ mRNA was detected by RT-PCR and IFN-γ protein expression was measured by ELISA. Proliferation and cell death in transfected SKOV-3 cells were measured by methyl-thiazolyl tetrazolium (MTT) assay and Hoechst 33258 staining, respectively and compared with untransfected and empty vector-transfected cells. RESULTS: pEGFP-IFN-γ SKOV-3 cells efficiently expressed and secreted IFN-γ. They exhibited significantly decreased cellular proliferation when compared with control untransfected or empty vector-transfected cells (P < 0.05). The mode of cell death was primarily apoptosis. CONCLUSIONS: Stable expression of IFN-γ significantly inhibits the proliferation of ovarian carcinoma cells and has the potential to be used in clinical applications to treat ovarian carcinoma in the future.


Assuntos
Interferon gama/biossíntese , Interferon gama/genética , Neoplasias Ovarianas/genética , Neoplasias Ovarianas/metabolismo , Plasmídeos/genética , Plasmídeos/metabolismo , Morte Celular/genética , Processos de Crescimento Celular/genética , Linhagem Celular Tumoral , Feminino , Humanos , Neoplasias Ovarianas/patologia , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Transfecção/métodos
9.
Mol Biol Rep ; 38(3): 2155-60, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20857206

RESUMO

Oct4 is a transcription factor that is required for pluripotency during early embryogenesis and the maintenance of embryonic stem (ES) cell and pluripotent cell identity. miR-302, a cluster of eight microRNAs (miRNAs) that are expressed specifically in ES cells and pluripotent cells, is crucial for normal pluripotent cell self-renewal and pluripotency. But, the mechanism by which miR-302 participates in the core regulatory circuitry that controls self-renewal and pluripotency in P19 embryonic carcinoma cells has not been established. Here, we show that Oct4 is required for the expression and transcriptional activation of miR-302 and that Oct4 binds to the putative promoter of miR-302, suggesting that Oct4 activates the primary miR-302 transcript in P19 cells. This study proposes that the miR-302 cluster acts downstream of the Oct4 regulation network in P19 cells.


Assuntos
Células-Tronco de Carcinoma Embrionário/metabolismo , Regulação Neoplásica da Expressão Gênica , MicroRNAs/genética , Família Multigênica , Fator 3 de Transcrição de Octâmero/metabolismo , Animais , Linhagem Celular Tumoral , Imunoprecipitação da Cromatina , Humanos , Camundongos , MicroRNAs/metabolismo , Regiões Promotoras Genéticas/genética , Ligação Proteica , Transcrição Gênica
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