RESUMO
BACKGROUND: Thromboelastography (TEG) is a widely utilized clinical testing method for real-time monitoring of platelet function and the thrombosis process. Lipid metabolism disorders are crucial risk factors for thrombosis. The lipid metabolism characteristics of hamsters resemble those of humans more closely than mice and rats, and their relatively large blood volume makes them suitable for studying the mechanisms of thrombosis related to plasma lipid mechanisms. Whole blood samples from golden Syrian hamsters and healthy humans were obtained following standard clinical procedures. TEG was employed to evaluate coagulation factor function, fibrinogen (Fib) function, platelet function, and the fibrinolytic system. METHODS: The whole blood from hamster or healthy human was isolated following the clinical procedure, and TEG was employed to evaluate the coagulation factor function, Fib function, platelet function, and fibrinolytic system. Coagulation analysis used ACLTOP750 automatic coagulation analysis pipeline. Blood routine testing used XN-2000 automatic blood analyzer. RESULTS: TEG parameters revealed that hamsters exhibited stronger coagulation factor function than humans (reaction time [R], p = 0.0117), with stronger Fib function (alpha angle, p < 0.0001; K-time [K], p < 0.0001). Platelet function did not differ significantly (maximum amplitude [MA], p = 0.077). Hamsters displayed higher coagulation status than humans (coagulation index [CI], p = 0.0023), and the rate of blood clot dissolution in hamsters differed from that in humans (percentage lysis 30 min after MA, p = 0.02). Coagulation analysis parameters indicated that prothrombin time (PT) and activated partial thromboplastin time (APTT) were faster in hamsters than in humans (PT, p = 0.0014; APTT, p = 0.03), whereas the Fib content was significantly lower in hamsters than in humans (p < 0.0001). No significant difference was observed in thrombin time (p = 0.1949). CONCLUSIONS: In summary, TEG could be used to evaluate thrombosis and bleeding parameters in whole blood samples from hamsters. The platelet function of hamsters closely resembled that of humans, whereas their coagulation function was significantly stronger.
RESUMO
The complexity and multiscale structure of coal pores significantly influence the gas diffusion and seepage characteristics of coal. To apply small angle X-ray scattering (SAXS) to study the coal pore structure parameters within the scale of 1-100 nm in the methane adsorption process, the X-ray window was optimized and a gas adsorption chamber was designed to interface with the small angle X-ray scattering platform. The fractal dimension and porosity of Hami coal samples under different methane pressures were studied using the small angle X-ray scattering platform and adsorption chamber. The surface and nanopore fractal information of the nanopores in coal were distinguished. The variation trends of the pores and surface fractal dimension with time under the same methane pressure were compared. The results indicate that the surface dimension changes from 2.56 to 2.75, and the extremum point may indicate that the primary nanopore structure is crushed by the adsorbed gas after approximately 15 minutes. This work clarifies that the fractal dimension can characterize the changes in nanopores in the process of gas adsorption by using SAXS. According to the fractal characteristics, the adsorption of gas in coal nanopores is summarized as four steps: expansion from adsorbance, deformation, crushing and recombination. The minimum porosity is 0.95% and the extreme value point is 1.47%. This work also shows that decrease in surface energy affect the porosity changes in nano-size pores. This work is of some significance to coalbed methane permeability improvement and gas extraction.
RESUMO
The great cormorant (Phalacrocorax carbo) is widely distributed in the world. Here, we report the complete mitogenome sequence of P. carbo, which was 18,995 bp long and composed of 15 protein-coding genes, 25 tRNA genes, 2 rRNA genes and 2 control regions. Most genes were located on H-strand except for 10 tRNAs and 2 ND6 genes. In protein-coding genes, ATG, ATC and GTG were used as initiation codons, and TAA, AGA, AGG and TAG were used as stop codons. The 25 tRNA genes ranged in size from 67 to 75 bp. And 12S and 16S rRNA were respectively 983 and 1517 bp. Except for a tRNA-Ala in P. carbo which was different from the tRNA-Lys annotated in P. chalconotus, both species of Phalacrocorax had the same order of other genes. In addition, a phylogenetic analysis may help us understand the evolutionary status of this widespread species better.
