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1.
Food Res Int ; 166: 112631, 2023 04.
Artigo em Inglês | MEDLINE | ID: mdl-36914308

RESUMO

The tingling evoked by Sichuan pepper and the burning elicited by chili pepper constitutes the typical flavor of Sichuan cuisine and is a component of leisure food. Although factors affecting the burning sensation have extensively been studied, few studies have examined the factors of individual sensitivity, personality traits, and dietary habits that contribute to the perception of oral tingling sensation, which hinders the formulation of tingling products and the development of new products. In contrast, many studies have examined the factors influencing the burning sensation. In this web-based survey, 68 participants disclosed their dietary habits, liking for tingling and hot foods, and psychological traits. Individual sensitivity to the tingling and burning sensation produced by a range of Sichuan pepper oleoresin and capsaicin solutions was determined using rated differences from control, generalized labeled magnitude scale method and ranking test. The consistency score indicated the accuracy of individual ranking results while also providing an indirect response to the sensitivity of the participant to supra-threshold for burning or tingling. Individual ratings for medium Sichuan pepper oleoresin concentrations significantly correlated with the just noticeable difference (p < 0.01), and ratings for medium and high capsaicin concentrations correlated significantly with 6-n-propylthiouracil ratings (p < 0.01). Notably, the power exponent of burning was significantly correlated with the burning recognition threshold (p < 0.01), and the power exponent of tingling and burning were significantly correlated (r = 0.340, p < 0.05). There was a negative correlation between supra-threshold tingling and burning sensation perceptions and life satisfaction ratings. Further, intensity ratings for oral tingling and burning sensation did not always correspond with individual sensitivity indicators (e.g., recognition threshold, 6-n-propylthiouracil, just noticeable difference, and consistency score). Thus, this study provides new insight into establishing a sensory selection method for chemesthetic sensation panelists and theoretical guidelines for formulation design and in-depth analysis of popular tingling dishes and foods.


Assuntos
Capsaicina , Estimulação Subliminar , Humanos , Propiltiouracila , Sensação/fisiologia , Comportamento Alimentar
2.
J Coll Physicians Surg Pak ; 32(2): 253-255, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35108804

RESUMO

The present study reports a case of intentional replantation (IR) for a right mandibular second premolar (#45). For the present case, root canal retreatment was first considered after removal of the metal post and core. When the metal post and core could not be removed from #45, micro-apical surgery or intentional tooth replantation was performed. Six-month postoperative evaluation revealed that the right mandibular second premolar had no obvious symptoms of discomfort, and the clinical follow-up revealed uneventful healing and good bone regeneration. The short-term clinical efficacy was acceptable. For cases with root canal treatment failure, when the apical surgical access could not be established due to the adjacent important anatomical structures, IR may be employed as an accepted endodontic treatment procedure. Key Words: Root canal treatment failure, Post-core crown, Root canal retreatment, Intentional replantation.


Assuntos
Tratamento do Canal Radicular , Reimplante Dentário , Dente Pré-Molar/cirurgia , Humanos , Retratamento , Resultado do Tratamento
3.
Food Res Int ; 149: 110660, 2021 11.
Artigo em Inglês | MEDLINE | ID: mdl-34600662

RESUMO

A cross-modal interaction may exist between the perception of saltiness and the pungency elicited by Sichuan pepper oleoresin (Spo). Thirty-one hypersensitive panelists were selected to participate in this study. Spo solutions dissolved in different NaCl concentrations, ranging from 1.25 g/L to 167.9 g/L, were used as the test samples. The rated difference from control, the generalized labeled magnitude scale (gLMS), and the time-intensity (TI) method were used to determine the detection threshold (DT), the recognition threshold (RT), the intensity, and the dynamic perception of pungent sensation. The results revealed that the pungency thresholds increased significantly (p < 0.01) in the solution with a high NaCl (167.9 g/L) concentration. Furthermore, high NaCl solutions suppressed the pungency intensity at all Spo concentrations except for 0.02 g Spo/L in water (p < 0.05). The TI and principal component analysis (PCA) results showed that an increase in the Spo concentration prolonged the duration of the pungency sensation. However, the maximum intensity, the time to reach maximum intensity, the decay time of perception, and the end time of perception of the Spo solutions ranging from 2.13 g/L to 4.69 g/L were significantly reduced at medium (42.95 g/L) and high NaCl concentrations. Since the salty and pungency sensations exhibited by NaCl and Spo are common flavor combinations in food products and dishes, studying the influence of saltiness on the dynamic perception of pungent sensation not only aids the development of oral cleaners during pungency evaluation but also presents significant theoretical and practical value in creating pungent food and cuisine based on consumer preferences.


Assuntos
Sensação , Cloreto de Sódio , Percepção , Extratos Vegetais , Verduras
4.
Shanghai Kou Qiang Yi Xue ; 30(2): 210-213, 2021 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-34109365

RESUMO

PURPOSE: To investigate the clinical effect of modified posterior dental splint in the treatment of anterior disk displacement without reduction of the temporomandibular joint (TMJ). METHODS: Eight-nine patients with anterior disk displacement without reduction of the TMJ who were treated in Stomatology Hospital of Guangzhou Medical University from October 2016 to October 2017 were selected in the study. 47 patients in the first group were treated by modified posterior dental splint, while 42 patients in the second group were treated by stabilization splint. The patients' pain index, mouth opening degrees were collected before treatment,3 months, 6 months, and 1 year after treatment. Statistical analysis of the data was performed using SPSS 18.0 software package, multivariate test was used to compare the indicators between the two groups. RESULTS: The effect of modified posterior dental splint on treating limited mouth opening was better than those of stabilization splint(P<0.05). In the treatment of joint pain, the two splints had no significant difference(P>0.05). CONCLUSIONS: Modified posterior dental splint is a new type of splint for the treatment of TMJ disorders. It is suitable for clinical application.


Assuntos
Luxações Articulares , Transtornos da Articulação Temporomandibular , Humanos , Luxações Articulares/terapia , Placas Oclusais , Contenções , Articulação Temporomandibular , Transtornos da Articulação Temporomandibular/terapia
5.
Food Res Int ; 136: 109581, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32846612

RESUMO

Health concerns related to the intake of salt have necessitated the investigation into NaCl reduction by examining the cross-modal interaction between the perception of saltiness and pungency elicited by Sichuan pepper oleoresin (Spo). The category scale and the generalized Labeled Magnitude Scale (gLMS) were used to determine the degree to which Spo enhanced the perception of saltiness in the NaCl model solution. Sixty-eight participants were divided into the hyposensitive, semi-sensitive, and hypersensitive groups according to individual exponent. The power functions of saltiness under different pungency carriers were obtained. The level of enhancement varied between the different sensitivity groups and pungency carriers. In the hypersensitive group, the low and strong pungency carriers effectively enhanced the perception of saltiness at low to moderate, and moderate to strong NaCl solutions, respectively. In the semi-sensitive group, low and moderate pungency carriers induced additive effect in the perception of saltiness at full and moderately strong NaCl solutions, respectively. However, the additive effect was inadequate in the hypo-sensitive group. Therefore, the low pungency solution was more feasible for enhancing in the perception of saltiness, while the maximum NaCl reduction percentages corresponded to the hypersensitive and semi-sensitive groups at 38.61% and 39.06%, respectively. This research not only provided insight into the effect of pungency on the perception of saltiness as it related to individual sensitivity, but also presented valuable information regarding flavor when developing food with reduced salt content.


Assuntos
Extratos Vegetais , Cloreto de Sódio , Humanos , Percepção , Paladar
6.
PLoS One ; 13(10): e0203840, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30278050

RESUMO

As an abundant post-translational modification, reversible phosphorylation is critical for the dynamic regulation of various biological processes. prkC, a critical serine/threonine-protein kinase in bacteria, plays important roles in regulation of signaling transduction. Identification of prkC-specific phosphorylation sites is fundamental for understanding the molecular mechanism of phosphorylation-mediated signaling. However, experimental identification of substrates for prkC is time-consuming and labor-intensive, and computational methods for kinase-specific phosphorylation prediction in bacteria have yet to be developed. In this study, we manually curated the experimentally identified substrates and phosphorylation sites of prkC from the published literature. The analyses of the sequence preferences showed that the substrate recognition pattern for prkC might be miscellaneous, and a complex strategy should be employed to predict potential prkC-specific phosphorylation sites. To develop the predictor, the amino acid location feature extraction method and the support vector machine algorithm were employed, and the methods achieved promising performance. Through 10-fold cross validation, the predictor reached a sensitivity of 91.67% at the specificity of 95.12%. Then, we developed freely accessible software, which is provided at http://free.cancerbio.info/prkc/. Based on the predictor, hundreds of potential prkC-specific phosphorylation sites were annotated based on the known bacterial phosphorylation sites. prkC-PSP was the first predictor for prkC-specific phosphorylation sites, and its prediction performance was promising. We anticipated that these analyses and the predictor could be helpful for further studies of prkC-mediated phosphorylation.


Assuntos
Bacillus subtilis/metabolismo , Proteínas Serina-Treonina Quinases/química , Proteínas Serina-Treonina Quinases/metabolismo , Algoritmos , Bacillus subtilis/química , Sítios de Ligação , Biologia Computacional/métodos , Curadoria de Dados , Fosforilação , Especificidade por Substrato , Máquina de Vetores de Suporte
7.
J Proteome Res ; 17(7): 2491-2498, 2018 07 06.
Artigo em Inglês | MEDLINE | ID: mdl-29882676

RESUMO

Stem-cell differentiation is a complex biological process controlled by a series of functional protein clusters and signaling transductions, especially metabolism-related pathways. Although previous studies have quantified the proteome and phosphoproteome for stem-cell differentiation, the investigation of acylation-mediated regulation is still absent. In this study, we quantitatively profiled the proteome, acetylome, and succinylome in pluripotent human embryonic stem cells (hESCs) and differentiated hepatocyte-like cells (HLCs). In total, 3843 proteins, 185 acetylation sites in 103 proteins, and 602 succinylation sites in 391 proteins were quantified. The quantitative proteome showed that in differentiated HLCs the TGF-ß, JAK-STAT, and RAS signaling pathways were activated, whereas ECM-related processes such as sulfates and leucine degradation were depressed. Interestingly, it was observed that the acetylation and succinylation were more intensive in hESCs, whereas protein processing in endoplasmic reticulum and the carbon metabolic pathways were especially highly succinylated. Because the metabolism patterns in pluripotent hESCs and the differentiated HLCs were different, we proposed that the dynamic acylations, especially succinylation, might regulate the Warburg-like effect and TCA cycle during differentiation. Taken together, we systematically profiled the protein and acylation levels of regulation in pluripotent hESCs and differentiated HLCs, and the results indicated the important roles of acylation in pluripotency maintenance and differentiation.


Assuntos
Diferenciação Celular , Células-Tronco Embrionárias Humanas/citologia , Proteoma/metabolismo , Acetilação , Acilação/fisiologia , Células Cultivadas , Hepatócitos/citologia , Hepatócitos/metabolismo , Células-Tronco Embrionárias Humanas/metabolismo , Humanos , Redes e Vias Metabólicas , Células-Tronco Pluripotentes/citologia , Células-Tronco Pluripotentes/metabolismo , Transdução de Sinais , Ácido Succínico/metabolismo
8.
China Journal of Endoscopy ; (12): 42-47, 2017.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-661539

RESUMO

Objective To analyze the recurrence and metastasis in patients with non-small cell lung cancer (NSCLC) after minimally invasive surgery. Methods 123 patients with NSCLC underwent thoracoscopic lobectomy from January 2008 to December 2013 were enrolled in the study. Their perioperative data and follow-up results were analyzed, and postoperative recurrence and metastasis were recorded. Multivariate logistics regression analysis was performed to investigate the influencing factors of postoperative recurrence and metastasis. Results The median operative time was 165 min (60 ~ 430 min) and the median intraoperative blood loss was 95 ml (20 ~ 3100 ml). Postoperative complications occurred in 15 cases (12.2%). All patients were followed up regularly after discharge, and the median follow-up time was 23.5 months (6 ~ 69 months). During the follow-up period, postoperative recurrence and metastasis occurred in 36 cases (29.3%) and 42 cases (34.1%), including 16 cases (13.0%) patients simultaneously appeared recurrence and metastasis. The ipsilateral lung (52.8%) was the most common site of recurrence, followed by mediastinal lymph nodes (38.9%). Bone (28.6%) was the most common site of metastasis,followed by contralateral lung (26.2%) and brain (19.0%). Multivariate logistic regression analysis showed that stage Ⅱ-Ⅲ, mediastinal lymph node metastasis and low differentiation were independent risk factors of postoperative recurrence (P < 0.05) while stage Ⅱ ~ Ⅲ , number of lymph node metastasis ≥ 3, without postoperative radiotherapy and chemotherapy were independent risk factors of postoperative metastasis (P < 0.05). Conclusions For patients with NSCLC, recurrence and metastasis after thoracoscopic lobectomy occurred mainly in the ipsilateral lung and bone. TNM staging, mediastinal lymph node metastasis, differentiation degree, lymph node metastases, postoperative radiotherapy and chemotherapy were related with postoperative recurrence and metastasis.

9.
China Journal of Endoscopy ; (12): 42-47, 2017.
Artigo em Chinês | WPRIM (Pacífico Ocidental) | ID: wpr-658620

RESUMO

Objective To analyze the recurrence and metastasis in patients with non-small cell lung cancer (NSCLC) after minimally invasive surgery. Methods 123 patients with NSCLC underwent thoracoscopic lobectomy from January 2008 to December 2013 were enrolled in the study. Their perioperative data and follow-up results were analyzed, and postoperative recurrence and metastasis were recorded. Multivariate logistics regression analysis was performed to investigate the influencing factors of postoperative recurrence and metastasis. Results The median operative time was 165 min (60 ~ 430 min) and the median intraoperative blood loss was 95 ml (20 ~ 3100 ml). Postoperative complications occurred in 15 cases (12.2%). All patients were followed up regularly after discharge, and the median follow-up time was 23.5 months (6 ~ 69 months). During the follow-up period, postoperative recurrence and metastasis occurred in 36 cases (29.3%) and 42 cases (34.1%), including 16 cases (13.0%) patients simultaneously appeared recurrence and metastasis. The ipsilateral lung (52.8%) was the most common site of recurrence, followed by mediastinal lymph nodes (38.9%). Bone (28.6%) was the most common site of metastasis,followed by contralateral lung (26.2%) and brain (19.0%). Multivariate logistic regression analysis showed that stage Ⅱ-Ⅲ, mediastinal lymph node metastasis and low differentiation were independent risk factors of postoperative recurrence (P < 0.05) while stage Ⅱ ~ Ⅲ , number of lymph node metastasis ≥ 3, without postoperative radiotherapy and chemotherapy were independent risk factors of postoperative metastasis (P < 0.05). Conclusions For patients with NSCLC, recurrence and metastasis after thoracoscopic lobectomy occurred mainly in the ipsilateral lung and bone. TNM staging, mediastinal lymph node metastasis, differentiation degree, lymph node metastases, postoperative radiotherapy and chemotherapy were related with postoperative recurrence and metastasis.

10.
Am J Transl Res ; 8(1): 196-208, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27069553

RESUMO

OBJECTIVE: Chronic kidney disease (CKD) predicted a poor prognosis in patients with coronary artery disease. There is a paucity of data on outcomes after revascularization in patients with chronic total occlusion (CTO) and CKD. This study aims to investigate the impact of CKD on the revascularization of CTO. METHODS: This study enrolled 1,092 CTO patients received treatments in our hospital between February 2009 and January 2014. Major adverse cardiac and cerebrovascular events (MACCE) and all-cause mortality were compared to evaluate medium- and long-term outcomes. Median follow-up was 39 months (interquartile range, 27-52 months). RESULT: CKD decreased cumulative MACCE-free survival rate (54.4 ± 6.2% vs. 70.9 ± 2.5%, P < 0.001) and cumulative survival rate (68.6 ± 6.3% vs. 90.5 ± 1.6%, P < 0.001). Revascularization was associated with better outcomes among patients with (MACCE-free survival rate: 64.8 ± 5.7% vs. 20.1 ± 15.3%, P = 0.009; survival rate 78.4 ± 5.6% vs. 38.7 ± 17.4%, P = 0.006) or without CKD (MACCE-free survival rate 73.9 ± 2.7% vs. 61.0 ± 5.4%, P = 0.001; survival rate 92.9 ± 1.5% vs. 83.8 ± 4.0%, P = 0.009). The benefit from revascularization was attenuated by CKD. Compared with percutaneous coronary intervention (PCI), coronary artery bypass grafting (CABG) had similar cumulative survival rates among patients, whether with or without CKD, but was associated with a higher cumulative MACCE-free survival rate (80.5 ± 3.4% vs. 68.5 ± 4.0%, P = 0.017) among patients without CKD. CONCLUSION: CKD attenuated the benefit from revascularization for CTO. Moreover, CABG was not superior to PCI among CTO patients, but with a reduction in MACCE in patients without CKD.

11.
Shanghai Kou Qiang Yi Xue ; 24(2): 224-7, 2015 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-25938155

RESUMO

PURPOSE: The purpose of this study was to compare the effect of two different flap designs on surgical removal of impacted mandibular third molars. METHODS: Four hundred patients who required removal of impacted mandibular third molars were included in the study. They were divided into group A and group B randomly. Patients in group A received distal incision flap and group B underwent angular incision flap. The effect of flap design on surgical time, incidence of dry socket, pain, swelling and trismus was evaluated postoperatively. Data analysis was carried out with SPSS 13.0 software package. RESULTS: The surgical time was longer in group A than in group B 3 days postoperatively. Facial swelling and trismus were less in group A than in group B 3 days postoperatively. There was no significant difference in two groups 7 days postoperatively. CONCLUSIONS: The distal incision flap design can release facial swelling and trismus in surgical removal of impacted mandibular third molars.


Assuntos
Dente Serotino , Retalhos Cirúrgicos , Extração Dentária , Dente Impactado , Resultado do Tratamento , Alvéolo Seco , Edema , Humanos , Mandíbula , Dor Pós-Operatória , Trismo
12.
Guang Pu Xue Yu Guang Pu Fen Xi ; 33(4): 1005-8, 2013 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-23841417

RESUMO

Upconversion nanoparticles (UCNPs) of small size have great potential in homogeneous assay based on fluorescence resonance energy transfer (FRET). A novel approach of surface ligand-exchange for preparing water-soluble and amido-functionalized NaYF4 : Er3+, Yb3+ UCNPs with 12 nm was developed. The ligand exchange process was confirmed by Fourier transform infrared spectra. Investigations by scan electron microscopy showed no obvious variations in the size and shape of the UCNPs. The circular dichroism characterization demonstrates that the secondary structure of the avidin remains largely intact after the conjugation. Avidin-biotin served as a bridge to make the short enough distance for FRET between the acceptor biotinylated R-phycoerythrin and the donors avidin-conjugated UCNPs. When the free biotins were added into this system, they competitively combined with avidin on the UCNPs surface and impede the FRET to triggered fluorescence changes. According to the relationship between this change and the addition of the amount of biotin, such FRET-based approach can reach a limit of detection in the nanomolar concentration range.

13.
Clin Oral Investig ; 17(9): 2003-10, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23358705

RESUMO

OBJECTIVE: The purpose of this study is to retrospectively explore the utilization of coralline hydroxyapatite in maxillary sinus augmentation. METHOD: One hundred and eighteen cases of sinus lift with coralline hydroxyapatite (CHA) were included in this study. In detail, simultaneous implantation was conducted in 78 patients (174 implants) and delayed implantation was done in 40 cases (82 implants) around 6 months after bone transplantation. The clinical features and X-ray radiographs after operation were analyzed to evaluate osseointegration procedures according to a planned medical follow-up. In the delayed group, around 6 months, a bone biopsy was taken just during implant placement in order to evaluate the new formed bone from a histological and histomorphometrical point of view. A further 6 months later, abutment connection was performed, and the patients received prosthetic restoration of the missing teeth. RESULT: Clinically, the incisions healed well. No abnormal reactions were found during follow-up period. All the 174 simultaneous implants were successful after 1-5 years of medical review; Out of 82 delayed implants, 3 were found to be loose. Histologically, all the specimens showed signs of active remodeling, and all the tissues had a large amount of osteocyte at sixth month after sinus augmentation. New bone formed dramatically. Radiologically, the density of CHA gradually reduced since the beginning of the third month, and CHA may be completely resolved at about fifth year. CONCLUSION: CHA is proven an ideal bone graft material for its reliable clinical results and favorable histocompatibility in the treatment of sinus atrophy or other kinds of insufficient bone volume in this region. Moreover, CHA's signal application can achieve desired clinical effect. CLINICAL RELEVANCE: This study shows the clinic application of CHA in maxillary sinus augmentation. Compared with popular mixture of autogenous bone and grafting materials, our results show CHA's signal application can achieve ideal osseointegration interface and satisfying clinic effect.


Assuntos
Cerâmica/farmacologia , Hidroxiapatitas/farmacologia , Levantamento do Assoalho do Seio Maxilar , Humanos , Estudos Retrospectivos
14.
J Craniomaxillofac Surg ; 40(6): 506-9, 2012 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21925891

RESUMO

A clinical investigation was conducted to demonstrate the epidemiology of cranio-facial injuries inflicted by animals in southern-central part of China-the World's most populous eastern country. It consisted of a retrospective study of 149 patients and was carried out in our department. All the patients were attacked by animals, such as buffalo, cattle, donkey, horse, dog, cat, and rat. There were 91 males and 58 females patients, aged from 5 to 65 years. 113 patients were from rural areas, and 36 from urban districts. Dogs, cattle, donkeys and buffalo, were the most common animals involved in this study. Bite was the predominant mechanism of injury, followed by kick, fall, trample, knock and scratch. The lip, chin and cheek were the commonest sites of soft tissue injury; Condylar process, symphysis, parasymphysis, and angle of mandible were the sites of fractures. Regarding the severity of injury, nearly half of the patients belonged to I and II classifications. The treatment results were satisfactory except for obvious scar formation, facial nerve palsy, and dysocclusion in a few patients. Facial injuries caused by animals are common in China, especially in rural areas. Timely treatment should be performed as soon as possible. Special attention should be paid to the prescription of antibiotics and vaccines. Psychotherapy may also be considered when necessary. It is suggested that adequate protective measures should be taken when animals are used for agricultural activities or kept as pets to minimize the incidence of facial injury.


Assuntos
Animais Domésticos , Traumatismos Faciais/epidemiologia , Adolescente , Adulto , Idoso , Animais , Mordeduras e Picadas/epidemiologia , Búfalos , Gatos , Bovinos , Criança , Pré-Escolar , China/epidemiologia , Cães , Equidae , Feminino , Cavalos , Humanos , Masculino , Fraturas Mandibulares/epidemiologia , Pessoa de Meia-Idade , Ratos , Estudos Retrospectivos , Saúde da População Rural/estatística & dados numéricos , Lesões dos Tecidos Moles/epidemiologia , Saúde da População Urbana/estatística & dados numéricos , Adulto Jovem
15.
J Craniofac Surg ; 22(4): 1460-2, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21772158

RESUMO

OBJECTIVE: The objective was to study the clinical application and effects of minimal incisions for treating zygomatic complex (ZMC) fractures. METHODS: A retrospective study was conducted on the 155 patients who underwent reduction of surgical injury by minimal incision for treating ZMC fractures. RESULTS: The outcomes after treatment were satisfactory functionally and aesthetically in most patients. The clinical results were good in 150 patients. No obvious scars were noticed in all the patients. After a follow-up of average 18 months, zygomatic asymmetry was present in 2 cases, numbness in the infraorbital area in 2 cases, and limitation of mouth opening in 1 patient. Plate removal was conducted in 3 patients. CONCLUSIONS: Repositioning of ZMC fractures via minimal incisions can get satisfactory results. With this kind of approach, the surgical injury can be reduced, and clear field of operation, convenient manipulation, less bleeding, high accuracy of reduction, and inconspicuous scar can be achieved as well. It is an ideal method and deserves to be recommended in clinical procedures.


Assuntos
Fixação Interna de Fraturas/métodos , Fraturas Zigomáticas/cirurgia , Adolescente , Adulto , Idoso , Placas Ósseas , Criança , Remoção de Dispositivo , Assimetria Facial/etiologia , Traumatismos do Nervo Facial/etiologia , Feminino , Seguimentos , Osso Frontal/cirurgia , Humanos , Hipestesia/etiologia , Luxações Articulares/cirurgia , Masculino , Maxila/cirurgia , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Órbita/inervação , Órbita/cirurgia , Complicações Pós-Operatórias , Hemorragia Pós-Operatória/etiologia , Amplitude de Movimento Articular/fisiologia , Estudos Retrospectivos , Infecção da Ferida Cirúrgica/etiologia , Osso Temporal/cirurgia , Resultado do Tratamento , Adulto Jovem
16.
Guang Pu Xue Yu Guang Pu Fen Xi ; 30(2): 435-9, 2010 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-20384140

RESUMO

In the present work, the CdSe/ZnSe core/shell quantum dots (QDs) were successfully transferred from organic phase to water phase via a two-phase solution system process by surface coating with amphiphilic polymer. Surface coating with amphiphilic polymer is an effective method, which can form stable soluble QDs in water. However, the conventional polymer coating method is performed in homogeneous phase, and it easily induces the aggregation of the QDs attributing to the long chain of enlace of the polymer. It is thus necessary and meaningful to develop surface coating technique for getting monodisperse coating QDs with amphiphilic polymer. In comparison with previously reported coating method, the authors' experiment process is performed in two-phase solution system, and can effectively reduces the possibility of aggregation of the QDs. The resulting hydrophilic CdSe/ZnSe core/shell QDs have long-term stability in water, and high quantum yield. The polymer coating process was affirmed by various characterizations. Fourier transform infrared spectra suggest that the octylamine modified polymer was successfully coated on the surface of the CdSe/ZnSe QDs. The transmission electron microscopy suggests that the size and shape of the QDs showed no obvious change before and after the coating process. Dynamic light scattering results indicate that the hydrophilic QDs exhibit narrow hydrodynamic size distribution with the mean hydrodynamic diameters of about 19.7 nm. The luminescence properties of the QDs were investigated with photoluminescence spectra and ultraviolet-visible absorption spectra. This polymer coating process has less effect on luminescence capability. The quantum yield decreased from 43% to 30%. Further, in order to confirm that the polymer capped QDs is biocompatible, the QDs were used for specific detection of the human IgG with fluorescence mapping. The specific molecular recognition capacity of goat anti-human IgG-modified QDs confirms that the polymer coated QDs have compatible functional chemical groups for bioconjugation and are suitable for biological applications.

17.
Guang Pu Xue Yu Guang Pu Fen Xi ; 30(1): 133-6, 2010 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-20302099

RESUMO

The authors synthesized a kind of upconversion nanocrystals NaYF4:Yb3+, Er3+ via the hydrothermal assisted homogeneous precipitation method, and then the nanocrystal was coated with silica. The SEM image demonstrated that the as-prepared samples were uniform in size distribution with ca. 25 nm before and ca. 250 nm after silica coating, respectively. The upconversion spectra and photoluminescence lifetime measurement showed that the silica shell had hardly effect on the properties of fluorescence of the NaYF4:Yb3+, Er3+ nanocrystals. At the same time, the naked eye-visible green upconversion fluorescence pattern was acquired from the as-prepared upconversion nanoparticles in the PBS buffer (2 wt%) excited by 980 nm laser at room temperature. These water-soluble nanoparticles were linked to the antibodies using the coupling reagents glutaraldehyde. The circular dichroism (CD) spectra of antibody and upconversion nanoparticles-antibody conjugates were very similar to each other, indicating that the secondary structure of antibody remained largely intact after the conjugation. Finally, antigen-antibody recognition reaction was performed on the surface of a silicon slide. The immunofluorescence in vitro indicated that the upconversion nanoparticles-antibody bioconjugates had excellent species-specific detection ability with hardly non-specific binding. Based on the present results, it is anticipated that the silica-coated upconversion nanoparticles are suitable for use as biolabeling materials.


Assuntos
Anticorpos/química , Nanopartículas/química , Dióxido de Silício/química , Coloração e Rotulagem/métodos , Imunofluorescência , Lasers
18.
Guang Pu Xue Yu Guang Pu Fen Xi ; 29(12): 3204-7, 2009 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-20210132

RESUMO

Photoluminescence quenching of colloidal CdSe core/shell quantum dots in the presence of hole transporting materials was studied by means of steady state and time resolved photoluminescence spectroscopy. With increasing hole transporting materials concentration in the CdSe core/shell quantum dot solution, the photoluminescence intensity and lifetime decreased gradually. The photoluminescence quenching of CdSe/ZnSe quantum dots with adding hole transporting material N,N'-bis(1-naphthyl)-N, N'-diphenyl-1,1 '-biphenyl-4, 4'-diamine (NPB) is more efficient than N,N'-diphenyl-N, N'-bis(3-methylphenyl)-1,1'-biphenyl-4,4'-diamine (TPD). And compared with CdSe core/shell quantum dots with ZnSe shell, the ZnS shell is an effective one on the surface of CdSe quantum dots for reducing photoluminescence quenching efficiency when interacting with hole transporting material TPD. Based on the analysis, there are two pathways in the photoluminescence quenching process: static quenching and dynamic quenching. The static quenching results from the decrease in the number of the emitting centers, and the dynamic quenching is caused by the hole transfer from quantum dots to hole transporting materials molecules. The efficiency of the photoluminescence quenching in CdSe core/shell quantum dots is strongly dependent on the structure of the shells and the HOMO levels of the hole transporting materials. The results are important for understanding the nature of quantum dots surface and the interaction of quantum dots and hole transporting materials.

19.
J Craniomaxillofac Surg ; 34(3): 182-5, 2006 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-16533601

RESUMO

OBJECTIVES: To study the value of coronal incisions for treating zygomatic complex fractures. PATIENTS AND METHODS: A retrospective study was conducted on 69 out of 83 patients regarding the indications and complications of scalp incisions for treating zygomatic complex fractures (the other 14 patients were treated by local incisions and approaches). RESULTS: In the early postoperative period, 5 patients suffered from haemorrhage, 2 had infections, 24 patients reported immediate postoperative anaesthesia and paraesthesia affecting the supraorbital region. Six had symptoms and signs of facial nerve injury: difficulty with wrinkling the forehead or to closing the eyes. After a follow-up of 3-5 years, 6 cases suffered from a scar wider than 0.5 cm, paraesthesia in 2 cases (parietal region and temporal region), depression of the temporal fossa in 2 and 1 patient had (persistent) palsy of the temporal branch of the facial nerve. CONCLUSION: On one hand, coronal incisions offer advantages such as: extensive exposure to ensure exact anatomical reduction. On the other hand, this incision has disadvantages such as obvious scars, long operating time, infections, haemorrhage, paraesthesia in the operative region, palsy of the facial nerve and depression of the temporal fossa. Therefore, the indications for coronal incisions should be strictly applied, and this incision should not be overused.


Assuntos
Parestesia/etiologia , Complicações Pós-Operatórias , Fraturas Zigomáticas/cirurgia , Adolescente , Adulto , Idoso , Traumatismos do Nervo Facial/etiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Radiografia , Estudos Retrospectivos , Fraturas Zigomáticas/diagnóstico por imagem
20.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 39(1): 12-4, 2004 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-14989859

RESUMO

OBJECTIVE: To investigate the effect of soft tissue incision and rigid internal fixation in the treatment of zygomatic fracture. METHODS: 70 cases with zygomatic fracture were entered into the study. The clinical data of 65 patients who were performed operations in the treatment were analyzed. The study focused on the incision, number and locations of Titanium plate. The facial contour, reduction,fixation, healing of incision, facial bisymmetry and complications of the operation were followed up 3 - 24 months after surgery. RESULTS: All the incisions were primary healing. Clinical and radiologic assessment of reduction was symmetric and stable in 91.5% of all cases, asymmetric in 8.5% of all cases. Subcutaneous hematoma happened in 5 cases, Ti plate exposure 1 in case, scalp scar width was more than 0.5 cm in 6 cases, frontal part or scalp paresthesia 8 cases. CONCLUSIONS: Coronal scalp incision has advantages such as good exposure, precise removal of the bone block and good symmetry of both sides. RIF can improve the stability of fracture, promotes fracture healing and guarantees the rehabilitation of zygomatic complex. direct reduction and rigid internal fixation with Titanium microplate or miniplate are satisfactory in zygomatic fracture.


Assuntos
Fixação Interna de Fraturas/métodos , Fraturas Zigomáticas/cirurgia , Adolescente , Adulto , Placas Ósseas , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Titânio
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