Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Front Plant Sci ; 15: 1327237, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38379942

RESUMO

Introduction: In order to solve the problem of precise identification and counting of tea pests, this study has proposed a novel tea pest identification method based on improved YOLOv7 network. Methods: This method used MPDIoU to optimize the original loss function, which improved the convergence speed of the model and simplifies the calculation process. Replace part of the network structure of the original model using Spatial and Channel reconstruction Convolution to reduce redundant features, lower the complexity of the model, and reduce computational costs. The Vision Transformer with Bi-Level Routing Attention has been incorporated to enhance the flexibility of model calculation allocation and content perception. Results: The experimental results revealed that the enhanced YOLOv7 model significantly boosted Precision, Recall, F1, and mAP by 5.68%, 5.14%, 5.41%, and 2.58% respectively, compared to the original YOLOv7. Furthermore, when compared to deep learning networks such as SSD, Faster Region-based Convolutional Neural Network (RCNN), and the original YOLOv7, this method proves to be superior while being externally validated. It exhibited a noticeable improvement in the FPS rates, with increments of 5.75 HZ, 34.42 HZ, and 25.44 HZ respectively. Moreover, the mAP for actual detection experiences significant enhancements, with respective increases of 2.49%, 12.26%, and 7.26%. Additionally, the parameter size is reduced by 1.39 G relative to the original model. Discussion: The improved model can not only identify and count tea pests efficiently and accurately, but also has the characteristics of high recognition rate, low parameters and high detection speed. It is of great significance to achieve realize the intelligent and precise prevention and control of tea pests.

2.
Int J Biol Markers ; 37(3): 296-305, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35722719

RESUMO

BACKGROUND: A whole-exome or targeted cancer genes panel by next-generation sequencing has been used widely in assisting individualized treatment decisions. Currently, multiple algorithms are developed to estimate DNA copy numbers based on sequencing data, which makes a comprehensive global glance at chromosomal integrity possible. We aim to classify gastric cancers based on chromosomal integrity to guide personalized therapy. METHODS: We investigated copy number variations (CNV) across the entire genome of 124 gastric carcinomas via exome or targeted sequencing. Chromosomal integrity was classified as chromosomal stability (CS), chromosomal instability (CIN) and intermediate state (CIN/CS) based on CNV results. Chromosomal integrity was correlated to molecular features and clinical characteristics. RESULTS: According the states of chromosomal integrity, gastric carcinomas can be stratified into two cohorts: CS and CIN. Our results showed a significant relationship between CIN status and TP53 mutation, but not RB1, phosphatase and tensin homolog (PTEN), or other reported DNA damage repair genes. The mutation frequency of the TP53 gene had great relevance. Our study initially revealed clinical significance of chromosomal integrity that CIN patients were prone to HER2-positive and mucinous adenocarcinoma, while CS patients were a diffuse subtype and poorly differentiated but had longer overall survival. CONCLUSIONS: We classified gastric carcinomas into two states of chromosomal integrity with clinical implications. The dichotomy is applicable to clinical transformation. We proposed that classifying gastric cancers based on chromosomal integrity would enable us to achieve personalized therapy for patients and may be beneficial to patient stratification in future clinical trials.


Assuntos
Adenocarcinoma Mucinoso , Neoplasias Gástricas , Instabilidade Cromossômica/genética , Variações do Número de Cópias de DNA/genética , Humanos , Mutação , Neoplasias Gástricas/genética , Neoplasias Gástricas/patologia
3.
Hum Mol Genet ; 30(22): 2110-2122, 2021 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-34196368

RESUMO

The well-established functions of UHRF1 converge to DNA biological processes, as exemplified by DNA methylation maintenance and DNA damage repair during cell cycles. However, the potential effect of UHRF1 on RNA metabolism is largely unexplored. Here, we revealed that UHRF1 serves as a novel alternative RNA splicing regulator. The protein interactome of UHRF1 identified various splicing factors. Among them, SF3B3 could interact with UHRF1 directly and participate in UHRF1-regulated alternative splicing events. Furthermore, we interrogated the RNA interactome of UHRF1, and surprisingly, we identified U snRNAs, the canonical spliceosome components, in the purified UHRF1 complex. Unexpectedly, we found H3R2 methylation status determines the binding preference of U snRNAs, especially U2 snRNAs. The involvement of U snRNAs in UHRF1-containing complex and their binding preference to specific chromatin configuration imply a finely orchestrated mechanism at play. Our results provided the resources and pinpointed the molecular basis of UHRF1-mediated alternative RNA splicing, which will help us better our understanding of the physiological and pathological roles of UHRF1 in disease development.


Assuntos
Processamento Alternativo , Proteínas Estimuladoras de Ligação a CCAAT/metabolismo , Histonas/metabolismo , Fatores de Processamento de RNA/metabolismo , RNA Nuclear Pequeno/genética , Ubiquitina-Proteína Ligases/metabolismo , Proteínas Estimuladoras de Ligação a CCAAT/genética , Humanos , Metilação , Complexos Multiproteicos , Conformação de Ácido Nucleico , Ligação Proteica , RNA Nuclear Pequeno/metabolismo , Ubiquitina-Proteína Ligases/genética
4.
Gastroenterology ; 153(6): 1607-1620, 2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-28780076

RESUMO

BACKGROUND & AIMS: Polycomb group proteins are epigenetic factors that silence gene expression; they are dysregulated in cancer cells and contribute to carcinogenesis by unclear mechanisms. We investigated whether BMI1 proto-oncogene, polycomb ring finger (BMI1), and polycomb group ring finger 2 (PCGF2, also called MEL18) are involved in the initiation and progression of colitis-associated cancer (CAC) in mice. METHODS: We generated mice containing floxed alleles of Bmi1 and/or Mel18 and/or Reg3b using the villin-Cre promoter (called Bmi1ΔIEC, Mel18ΔIEC, DKO, and TKO mice). We also disrupted Bmi1 and/or Mel18 specifically in intestinal epithelial cells (IECs) using the villin-CreERT2-inducible promoter. CAC was induced in cre-negative littermate mice (control) and mice with conditional disruption of Bmi1 and/or Mel18 by intraperitoneal injection of azoxymethane (AOM) followed by addition of dextran sulfate sodium (DSS) to drinking water. Colon tissues were collected from mice and analyzed by histology and immunoblots; IECs were isolated and used in cDNA microarray analyses. RESULTS: Following administration of AOM and DSS, DKO mice developed significantly fewer polyps than control, Bmi1ΔIEC, Mel18ΔIEC, Reg3bΔIEC, or TKO mice. Adenomas in the colons of DKO mice were low-grade dysplasias, whereas adenomas in control, Bmi1ΔIEC, Mel18ΔIEC, Reg3bΔIEC, or TKO mice were high-grade dysplasias with aggressive invasion of the muscularis mucosa. Disruption of Bmi1 and Mel18 (DKO mice) during late stages of carcinogenesis significantly reduced the numbers of large adenomas and the load of total adenomas, reduced proliferation, and increased apoptosis in colon tissues. IECs isolated from DKO mice after AOM and DSS administration had increased expression of Reg3b compared with control, Bmi1ΔIEC, or Mel18ΔIEC mice. Expression of REG3B was sufficient to inhibit cytokine-induced activation of STAT3 in IECs. The human REG3ß protein, the functional counterpart of mouse REG3B, inhibited STAT3 activity in human 293T cells, and its expression level in colorectal tumors correlated inversely with pSTAT3 level and survival times of patients. CONCLUSIONS: BMI1 and MEL18 contribute to the development of CAC in mice by promoting proliferation and reducing apoptosis via suppressing expression of Reg3b. REG3B negatively regulates cytokine-induced activation of STAT3 in colon epithelial cells. This pathway might be targeted in patients with colitis to reduce carcinogenesis.


Assuntos
Pólipos Adenomatosos/etiologia , Transformação Celular Neoplásica/metabolismo , Colite/complicações , Colo/enzimologia , Neoplasias do Colo/etiologia , Pólipos do Colo/etiologia , Mucosa Intestinal/enzimologia , Proteínas Associadas a Pancreatite/metabolismo , Complexo Repressor Polycomb 1/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Fator de Transcrição STAT3/metabolismo , Pólipos Adenomatosos/enzimologia , Pólipos Adenomatosos/genética , Pólipos Adenomatosos/patologia , Animais , Apoptose , Fatores de Coagulação Sanguínea/genética , Fatores de Coagulação Sanguínea/metabolismo , Proliferação de Células , Transformação Celular Neoplásica/genética , Transformação Celular Neoplásica/patologia , Colite/enzimologia , Colite/genética , Colite/patologia , Colo/patologia , Neoplasias do Colo/enzimologia , Neoplasias do Colo/genética , Neoplasias do Colo/patologia , Pólipos do Colo/enzimologia , Pólipos do Colo/genética , Pólipos do Colo/patologia , Modelos Animais de Doenças , Progressão da Doença , Predisposição Genética para Doença , Células HEK293 , Humanos , Mucosa Intestinal/patologia , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fenótipo , Fosforilação , Complexo Repressor Polycomb 1/deficiência , Complexo Repressor Polycomb 1/genética , Proto-Oncogene Mas , Proteínas Proto-Oncogênicas/deficiência , Proteínas Proto-Oncogênicas/genética , Proteínas de Ligação a RNA , Proteínas Ribossômicas , Transdução de Sinais , Fatores de Tempo
5.
Acta Biochim Biophys Sin (Shanghai) ; 44(1): 40-7, 2012 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22194012

RESUMO

Traditionally, we know that genomic DNA will produce transcripts named messenger RNA and then translate into protein following the instruction of genetic central dogma, and RNA works here as a pass-by messenger. Now increasing evidence shows that RNA is a key regulator as well as a message transmitter. It is discovered by next-generation sequencing techniques that most genomic DNA are generally transcribed to non-coding RNA, highly beyond the percentage of coding mRNA. These non-coding RNAs (ncRNAs), belonging to several groups, have critical roles in many cellular processes, expanding our understanding of the RNA world. We review here the different categories of ncRNA according to genome location and how ncRNAs guide and recruit chromatin modification complex to specific loci of genome to modulate gene expression by affecting chromatin state.


Assuntos
Montagem e Desmontagem da Cromatina/fisiologia , Cromatina/metabolismo , RNA não Traduzido/metabolismo , Epigênese Genética , Regulação da Expressão Gênica , Regiões Promotoras Genéticas/fisiologia , RNA Interferente Pequeno/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...