Progress in long non-coding RNAs as prognostic factors of papillary thyroid carcinoma.

Papillary thyroid carcinoma (PTC) is generally recognized as a slow-growing tumor. However, a small subset of patients may still experience relapse or metastasis shortly after therapy, leading to a poor prognosis and raising concerns about excessive medical treatment. One major challenge lies in the inadequacy of effective biomarkers for accurate risk stratification. Long non-coding RNAs (lncRNAs), which are closely related to malignant characteristics and poor prognosis, play a significant role in the genesis and development of PTC through various pathways. The objective of this review is to provide a comprehensive summary of the biological functions of lncRNAs in PTC, identify prognosis-relevant lncRNAs, and explore their potential mechanisms in drug resistance to BRAF kinase inhibitors, tumor dedifferentiation, and lymph node metastasis. By doing so, this review aims to offer valuable references for both basic research and the prediction of PTC prognosis.

Regulation of the Ice â to Ice III high pressure phase transition meta-stability in milk and its bactericidal effects.

This study was focused on a novel approach of creating perturbations under high pressure (HP) meta-stable Ice Ⅰ to Ice Ⅲ phase transition and its bactericidal effects. Experiments were carried out under subzero high pressure processing conditions using Escherichia coli suspended in milk, and the microbial inactivation before and after the meta-stable state regulation was compared. The phase transition position of unperturbed milk was 302 MPa/-37.5 °C. The volume change resulting from the phase transition was employed as the perturbation mechanism. Glucose (5 %, 20 %) and sodium chloride solutions (5 %, 20 %) were used as regulatory sources. Glucose solutions accelerated the phase change of the milk better than the sodium chloride solution and resulted in an optimum phase transition position of milk at 243 MPa/-30.6 °C. The induced perturbations accelerated meta-stable transformation and enhanced the microbial destruction. At 330 MPa/3s, compared to the unfrozen samples, the lethality of E. coli in the frozen-regulated samples significantly increased by 1.79 log. The relationship between the E. coli inactivation within the phase change pressure range and the pressure was not continuous, but a segmented one, both before and after meta-stable state regulation. A higher level of E. coli destruction was accomplished by a 5 min pressure-holding of frozen samples at 220 MPa and 280 MPa as compared to the one-pulse and two-pulses treatments without holding time. The maximum lethality of 6.73 log was achieved at 280 MPa/5 min in the frozen-regulated application.

Biodegradation of penicillin G sodium by Sphingobacterium sp. SQW1: Performance, degradation mechanism, and key enzymes.

Biodegradation is an efficient and cost-effective approach to remove residual penicillin G sodium (PGNa) from the environment. In this study, the effective PGNa-degrading strain SQW1 (Sphingobacterium sp.) was screened from contaminated soil using enrichment technique. The effects of critical operational parameters on PGNa degradation by strain SQW1 were systematically investigated, and these parameters were optimized by response surface methodology to maximize PGNa degradation. Comparative experiments found the extracellular enzyme to completely degrade PGNa within 60 min. Combined with whole genome sequencing of strain SQW1 and LC-MS analysis of degradation products, penicillin acylase and ß-lactamase were identified as critical enzymes for PGNa biodegradation. Moreover, three degradation pathways were postulated, including ß-lactam hydrolysis, penicillin acylase hydrolysis, decarboxylation, desulfurization, demethylation, oxidative dehydrogenation, hydroxyl reduction, and demethylation reactions. The toxicity of PGNa biodegradation intermediates was assessed using paper diffusion method, ECOSAR, and TEST software, which showed that the biodegradation products had low toxicity. This study is the first to describe PGNa-degrading bacteria and detailed degradation mechanisms, which will provide new insights into the PGNa biodegradation.

Comprehensive investigation of pressure-induced gelatinization of starches using in situ and ex-situ technical analyses.

The pressure-induce gelatinization of pea starch, potato starch and corn starch was investigated by a combination of in situ and ex-situ technical analyses. According to in-situ observation of gelatinization process and the analysis of granular morphology by scanning electronic microscopy (SEM), the pressure that caused potato starch gelatinization was the highest at 600 MPa. This was followed by pea starch, and the pressure that caused the gelatinization of corn starch was the lowest at 400 MPa. In situ Raman spectral analysis revealed the molecular mechanism of starch gelatinization. This indicated that high pressure treatment resulted in the modification of the structure of the double helical polymers and the degree of a double helix of the starch crystalline varied as well. This study dynamically monitors the starch gelatinization process, aiming to better understand the gelatinization mechanism and provide a theoretical basis for the application of pressure in the starch field.

Effects of hygroscopicity on aerosol optical properties and direct radiative forcing in Beijing: Based on two-year observations.

The influence of relative humidity on aerosol properties and the direct radiative forcing of PM10 and PM1 were investigated in Beijing from January 2018 to December 2019. The annual mean scattering hygroscopic growth factor at RH = 80 % [f(80 %)] of PM10 and PM1 were 1.60 ± 0.24 and 1.58 ± 0.22, respectively. The variation of aerosol hygroscopic growth factors of PM10 and PM1 aerosols was similar, which is mainly due to the fact that aerosol scattering in Beijing is dominated by fine particles. The seasonal mean f(80 %) of PM10 from spring to winter were 1.66 ± 0.23, 1.71 ± 0.25, 1.51 ± 0.20, 1.49 ± 0.16, respectively, which were higher in spring and summer, and lower in autumn and winter. The diurnal variation of f(80 %) was relatively higher from 12:00 to 18:00, which could be related to the formation of secondary aerosols by photochemical reactions. f(80 %) shows a strong positive relationship with both the scattering Angström exponent (SAE) and the single scattering albedo (ω0) under dry conditions; therefore, the scattering hygroscopic growth factor could be estimated using these two parameters. The upscatter fraction (ß) and single scattering albedo, which are the key aerosol optical properties for the calculation of direct radiative forcing, are also RH-dependent. As RH increases, the upscatter fraction (backscatter fraction) decreases and ω0 increases. The aerosol radiative forcing at RH 80 % was 1.48 times as that in the dry state. The sensitivity experiment showed that the variation in the scattering coefficient with relative humidity had the greatest influence on radiation forcing, followed by ß and ω0. The seasonal variation of ΔF(80 %)/ΔF(dry) coincides with that of the aerosol hygroscopic growth factor. Our study suggests that understanding the influence of relative humidity on aerosol properties and direct radiative forcing is important for accurately estimating the radiative forcing of aerosols.

Atmospheric Particle Hygroscopicity and the Influence by Oxidation State of Organic Aerosols in Urban Beijing.

A hygroscopic tandem differential mobility analyser (H-TDMA) was used to observe the size-resolved hygroscopic characteristics of submicron particles in January and April 2018 in urban Beijing. The probability distribution of the hygroscopic growth factor (HGF-PDF) in winter and spring usually showed a bimodal pattern, with more hygroscopic mode (MH) being more dominant. The seasonal variation in particle hygroscopicity was related to the origin of air mass, which received polluted southerly air masses in spring and clean northwesterly air masses in winter. Particles showed stronger hygroscopic behaviour during heavy pollution episodes (HPEs) with elevated concentrations of secondary aerosols, especially higher mass fraction of nitrate, which were indicated using the PM2.5 (particulate matter with diameter below 2.5 µm) mass concentration normalised by CO mass concentration. The hygroscopic parameter (κ) values were calculated using H-TDMA (κhtdma) and chemical composition (κchem). The closure study showed that κchem was overestimated in winter afternoon when compared with κhtdma, because the organic particle hygroscopic parameter (κorg) was overestimated in the calculations. It was influenced by the presence of a high concentration of hydrocarbon-like organic aerosol (HOA) with a weak water uptake ability. A positive relationship was observed between κorg and the ratio of oxygenated organic aerosol (OOA) and HOA, thereby indicating that the strong oxidation state enhanced the hygroscopicity of the particles. This study revealed the effect of local emission sources and secondary aerosol formation processes on particle hygroscopicity, which is of great significance for understanding the pollution formation mechanism in the North China Plain.

Recent Progress in the Synergistic Bactericidal Effect of High Pressure and Temperature Processing in Fruits and Vegetables and Related Kinetics.

BACKGROUND: Traditional thermal processing is a widely used method to ensure food safety. However, thermal processing leads to a significant decline in food quality, especially in the case of fruits and vegetables. To overcome this drawback, researchers are extensively exploring alternative non-thermal High-Pressure Processing (HPP) technology to ensure microbial safety and retaining the sensory and nutritional quality of food. However, HPP is unable to inactivate the spores of some pathogenic bacteria; thus, HPP in conjunction with moderate- and low-temperature is employed for inactivating the spores of harmful microorganisms. Scope and approach: In this paper, the inactivation effect of high-pressure and high-pressure thermal processing (HPTP) on harmful microorganisms in different food systems, along with the bactericidal kinetics model followed by HPP in certain food samples, have been reviewed. In addition, the effects of different factors such as microorganism species and growth stage, process parameters and pressurization mode, and food composition on microbial inactivation under the combined high-pressure and moderate/low-temperature treatment were discussed. KEY FINDINGS AND CONCLUSIONS: The establishment of a reliable bactericidal kinetic model and accurate prediction of microbial inactivation will be helpful for industrial design, development, and optimization of safe HPP and HPTP treatment conditions.

Study on the Influence of Defects on Fracture Mechanical Behavior of Cu/SAC305/Cu Solder Joint.

The fracture behavior of the Cu/Sn-3.0Ag-0.5Sn (SAC305)/Cu solder joint was investigated by conducting tensile tests with in situ X-ray micro-computed tomography (µ-CT) observation, and finite element (FE) simulation. The tensile fracture process of solder joints with a real internal defect structure was simulated and compared with the experimental results in terms of defect distribution and fracture path. Additionally, the stress distribution around the defects during the tensile process was calculated. The experimental results reveal that the pores near the intermetallic compound (IMC) layers and the flaky cracks inside the solder significantly affected the crack path. The aggregation degree of the spherical pores and the angle between the crack surface and the loading direction controlled the initiation position and propagation path of the cracks. The fracture morphology indicates that the fracture of the IMC layer was brittle, while the solder fracture exhibited ductile tearing. There are significant differences in the fracture morphology under tensile and shear loading.

Biodegradation of chlortetracycline by Bacillus cereus LZ01: Performance, degradative pathway and possible genes involved.

Microbial degradation of chlortetracycline (CTC) is an effective bioremediation method. In the present study, an enrichment technique was used to isolate a Bacillus cereus LZ01 strain capable of effectively degrading CTC from cattle manure. Response surface methodology was used to identify optimized conditions under which strain LZ01 was able to achieve maximal CTC removal (83.58%): temperature of 35.77 °C, solution pH of 7.59, CTC concentration of 57.72 mg/L and microbial inoculum of 0.98%. The antibacterial effect of CTC degradation products on Escherichia coli was investigated by the disk diffusion test, revealing that the products by LZ01 degradation of CTC exhibited lower toxicity than parent compound. Shake flask batch experiments showed that the biodegradation of CTC was a synergistic effect of intracellular and extracellular enzymes, and intracellular enzyme had a better degradation effect on CTC (77.56%). Whole genome sequencing revealed that genes associated with ring-opening hydrolysis, demethylation, deamination and dehydrogenation in strain LZ01 may be involved in the biodegradation of CTC. Subsequent seven possible biodegradation products were identified by LC-MS analyses, and the biodegradation pathways were proposed. Overall, this study provides a theoretical foundation for the characterization and mechanism of CTC degradation in the environment by Bacillus cereus LZ01.

Effect of high hydrostatic pressure on activity, thermal stability and structure of horseradish peroxidase.

The mechanism of the high hydrostatic pressure (HHP) effect on horseradish peroxidase (HRP) is still unclear. The activity, thermal stability and structural changes of HRP after HHP treatments were studied in this work. Compared with the untreated sample, the enzyme activity reduces by 36% after 800 MPa processing. The results indicated that the conformation of the enzyme active center changes under pressure. Furthermore, HHP also changes the conformation of disulfide bonds and some secondary structures in HRP. These structural and conformational changes induce decreased activity. In addition, differential thermal scanning (DSC) results showed that the thermal denaturation temperature decreased from 103.74 °C to 85.78 °C after pressure treatment, suggesting HRP molecules formed large aggregates after pressure treatment. In this study, the interaction mechanism between pressure and enzyme was studied as well, and the results can provide some guidance for the application of HHP technology in fruit and vegetable products processing.

Fine-tuning grain amylose contents by genome editing of Waxy cis-regulatory region in rice.

Rice grain amylose contents (ACs) is a key quantitative trait influencing eating and cooking quality. Regulating the expression level of Waxy, a key gene controlling ACs, and in turn fine-tuning the grain ACs, is an ideal approach to improve grain quality of rice varieties. Based on CRISPR/Cas9 genome editing technology, we designed eight targets in the cis-regulatory region of Wxa background, screened phenotypic changes of the transgenic lines and generated eight novel Waxy alleles with altered grain ACs. Among the eight alleles, we found that a 407-bp non-homologous substitution (NHS) in the 5'UTR-intron caused by genome editing regulated Waxy expression and decreased grain ACs by 2.9%. Moreover, embedding the 407-bp NHS into the cis-regulatory region of Wxb allele can also affect gene activity. Our work suggested the effect of 5'UTR-intron on Waxy gene expression regulation, and provided a potentially useful allele in breeding that can finely adjust rice grain ACs.

Long-term trend of new particle formation events in the Yangtze River Delta, China and its influencing factors: 7-year dataset analysis.

To evaluate the influence of anthropogenic emission reductions since 2013 in China, a long-term trend analysis of the particle number size distribution (PNSD) and new particle formation (NPF) events in the Yangtze River Delta (YRD) region was conducted based on the PNSD measurement (diameter ranging from 3 to 850 nm) at the Lin'an (LAN) regional background station from 2013 to 2019. A modified Mann-Kendall test and a Theil-Sen estimator were used to calculate the overall trend of particle number concentrations in different modes and the relevant influencing factors. We observed a significant decreasing trend in the Aitken and accumulation mode number concentrations, with annual decrease rates of approximately 5.6% and 8.2%, respectively, resulting in an approximately 6.0% decline in total particles annually. However, the nucleation mode particle number concentration showed no significant trend from 2013 to 2016, but an increasing trend from 2016 to 2019, which was related to the NPF events occurrence frequency. The regional NPF events of "banana shape" accounted for an increasing fraction of all NPF events. As a key parameter influencing the NPF event, the condensation sink decreased by approximately 63% from 2013 to 2019. Moreover, the estimated sulfuric acid concentration decreased by approximately 50%, with a higher reduction rate occurring during 2013-2016 as result of the effective SO2 reduction. Surface meteorological factors (including the air temperature, relative humidity, air pressure, and wind) and the air masses origin were found to played minor roles in the long-term trend of NPF events. As PNSD and NPF events are closely related to changes in the particle emissions and regional air pollution levels, studies concerning PNSD and NPF are necessary to provide important information regarding air quality improvements and evaluating the efficacy of climate change mitigation strategies.

High-pressure in situ methods revealing the effect of pressure on glutathione structure.

High-pressure processing is a nonthermal method of food processing that is widely used in sterilization and enzyme inactivation. Although some works on technological parameters and quality evaluation have been performed, the mechanism of high pressure on food is still unclear. Due to the complexity of food ingredients, a tremendously important tripeptide in food proteins, orthorhombic l-glutathione, was employed in this work. In addition, in situ methods such as high-pressure Raman, infrared, and X-ray diffraction were used to investigate the structural changes in the pressure range of 0-10 GPa. Experimental results showed that the sample underwent two phase transitions in pressure intervals of 1.8-2.2 and 4.1-5.3 GPa. In addition, the strength of the hydrogen bonds (NH⋯O; OH⋯O; SH⋯O; CH⋯O) also changed in the two pressure intervals. This work may have potential research value for revealing the mechanism of high-pressure processing on food proteins.

Simultaneous measurements of PM1 and PM10 aerosol scattering properties and their relationships in urban Beijing: A two-year observation.

The aerosol scattering properties of submicron (PM1) and sub-10 µm particles (PM10) under dry conditions (RH <30%) were investigated in Beijing from 2018 to 2019. Using the simultaneous measurement of PM1 and PM10, the scattering properties of super micron (PM10-1) particles were also calculated. At 550 nm, the average of scattering coefficient (σsp) and asymmetry parameter (g) were 208.7 ± 204.9 Mm-1 and 0.61 ± 0.04 for PM10, 140.6 ± 130.2 Mm-1 and 0.60 ± 0.04 for PM1, and 69.8 ± 82.2 Mm-1 and 0.62 ± 0.04 for PM10-1, respectively, while the backscattering ratio (b) values were 0.13 ± 0.02 for PM10 and PM1, and 0.12 ± 0.02 for PM10-1. The mass scattering efficiencies (MSE) for PM10, PM1 and PM10-1 were 2.43 ± 2.37, 3.67 ± 0.96, and 1.73 ± 1.82 m2 g-1, respectively. In 2019, σsp decreased by approximately 18.4% for PM10, and 16.7% for PM1 compared with those in 2018, which was quite similar to the decrease of 17% and 19% for PM10 and PM2.5 mass concentrations during the same time period. The scattering Ångström exponent (SAE450/700), which was 1.88 ± 0.29 for PM1 and 1.50 ± 0.27 for PM10 indicated size distributions dominated by fine mode aerosols. This is also evidenced by the high submicron scattering ratio (Rsp) (71.1% ± 7.9%). The high SAE, Rsp, and high PM1 σsp in the study suggest that control of fine particle pollution is important to reduce overall PM pollution in urban Beijing. In addition, with an increase in σsp, b, Rsp, and SAE gradually decreased, while g and MSE increased. The clearly scattering coefficient-dependent MSE suggests that high aerosol loading and high MSE both play an important role in degraded visibility during heavy pollution periods.

Evaluation of Local Skeletal Muscle Blood Flow in Manipulative Therapy by Diffuse Correlation Spectroscopy.

Manipulative therapy (MT) is applied to motor organs through a therapist's hands. Although MT has been utilized in various medical treatments based on its potential role for increasing the blood flow to the local muscle, a quantitative validation of local muscle blood flow in MT remains challenging due to the lack of appropriate bedside evaluation techniques. Therefore, we investigated changes in the local blood flow to the muscle undergoing MT by employing diffuse correlation spectroscopy, a portable and emerging optical measurement technology that non-invasively measures blood flow in deep tissues. This study investigated the changes in blood flow, heart rate, blood pressure, and autonomic nervous activity in the trapezius muscle through MT application in 30 volunteers without neck and shoulder injury. Five minutes of MT significantly increased the median local blood flow relative to that of the pre-MT period (p < 0.05). The post-MT local blood flow increase was significantly higher in the MT condition than in the control condition, where participants remained still without receiving MT for the same time (p < 0.05). However, MT did not affect the heart rate, blood pressure, or cardiac autonomic nervous activity. The post-MT increase in muscle blood flow was significantly higher in the participants with muscle stiffness in the neck and shoulder regions than in those without (p < 0.05). These results suggest that MT could increase the local blood flow to the target skeletal muscle, with minimal effects on systemic circulatory function.

Neonicotinoid and sulfoximine pesticides differentially impair insect escape behavior and motion detection.

Insect nervous systems offer unique advantages for studying interactions between sensory systems and behavior, given their complexity with high tractability. By examining the neural coding of salient environmental stimuli and resulting behavioral output in the context of environmental stressors, we gain an understanding of the effects of these stressors on brain and behavior and provide insight into normal function. The implication of neonicotinoid (neonic) pesticides in contributing to declines of nontarget species, such as bees, has motivated the development of new compounds that can potentially mitigate putative resistance in target species and declines of nontarget species. We used a neuroethologic approach, including behavioral assays and multineuronal recording techniques, to investigate effects of imidacloprid (IMD) and the novel insecticide sulfoxaflor (SFX) on visual motion-detection circuits and related escape behavior in the tractable locust system. Despite similar LD50 values, IMD and SFX evoked different behavioral and physiological effects. IMD significantly attenuated collision avoidance behaviors and impaired responses of neural populations, including decreases in spontaneous firing and neural habituation. In contrast, SFX displayed no effect at a comparable sublethal dose. These results show that neonics affect population responses and habituation of a visual motion detection system. We propose that differences in the sublethal effects of SFX reflect a different mode of action than that of IMD. More broadly, we suggest that neuroethologic assays for comparative neurotoxicology are valuable tools for fully addressing current issues regarding the proximal effects of environmental toxicity in nontarget species.

Opposite monosynaptic scaling of BLP-vCA1 inputs governs hopefulness- and helplessness-modulated spatial learning and memory.

Different emotional states lead to distinct behavioural consequences even when faced with the same challenging events. Emotions affect learning and memory capacities, but the underlying neurobiological mechanisms remain elusive. Here we establish models of learned helplessness (LHL) and learned hopefulness (LHF) by exposing animals to inescapable foot shocks or with anticipated avoidance trainings. The LHF animals show spatial memory potentiation with excitatory monosynaptic upscaling between posterior basolateral amygdale (BLP) and ventral hippocampal CA1 (vCA1), whereas the LHL show memory deficits with an attenuated BLP-vCA1 connection. Optogenetic disruption of BLP-vCA1 inputs abolishes the effects of LHF and impairs synaptic plasticity. By contrast, targeted BLP-vCA1 stimulation rescues the LHL-induced memory deficits and mimics the effects of LHF. BLP-vCA1 stimulation increases synaptic transmission and dendritic plasticity with the upregulation of CREB and intrasynaptic AMPA receptors in CA1. These findings indicate that opposite excitatory monosynaptic scaling of BLP-vCA1 controls LHF- and LHL-modulated spatial memory, revealing circuit-specific mechanisms linking emotions to memory.

Expression, purification, and evaluation for anticancer activity of ribosomal protein L31 gene (RPL31) from the giant panda (Ailuropoda melanoleuca).

Ribosomal protein L31 gene is a component of the 60S large ribosomal subunit encoded by RPL31 gene, while ribosomal protein L31 (RPL31) is an important constituent of peptidyltransferase center. In our research, the cDNA and the genomic sequence of RPL31 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology respectively, following sequencing and analyzing preliminarily. We constructed a recombinant expression vector contained RPL31 cDNA and over-expressed it in Escherichia coli using pET28a plasmids. The expression product was purified to obtain recombinant protein of RPL31 from the giant panda. Recombinant protein of RPL31 obtained from the experiment acted on human laryngeal carcinoma Hep-2 and human hepatoma HepG-2 cells for study of its anti-cancer activity by MTT [3-(4, 5-dimehyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide] method. Then observe these cells growth depressive effect. The result indicated that the cDNA fragment of the RPL31 cloned from the giant panda is 419 bp in size, containing an open reading frame of 378 bp, and deduced protein was composed of 125 amino acids with an estimated molecular weight of 14.46-kDa and PI of 11.21. The length of the genomic sequence is 8,091 bp, which was found to possess four exons and three introns. The RPL31 gene can be readily expressed in E.coli, expecting 18-kDa polypeptide that formed inclusion bodies. Recombinant protein RPL31 from the giant panda consists of 157 amino acids with an estimated molecular weight of 17.86 kDa and PI of 10.77. The outcomes showed that the cell growth inhibition rate in a time- and dose-dependent on recombinant protein RPL31. And also indicated that the effect at low concentrations was better than high concentrations on Hep-2 cells, and the concentration of 0.33 µg/mL had the best rate of growth inhibition, 44 %. Consequently, our study aimed at revealing the recombinant protein RPL31 anti-cancer function from the giant panda, providing scientific basis and resources for the research and development of cancer protein drugs anti-cancer mechanism research. Further studies of the mechanism and the signal transduction pathways are in progress.

cDNA cloning, overexpression, purification and pharmacologic evaluation for anticancer activity of ribosomal protein L23A gene (RPL23A) from the Giant Panda.

RPL23A gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L23P family of ribosomal proteins, which is located in the cytoplasm. The purpose of this paper was to explore the structure and anti-cancer function of ribosomal protein L23A (RPL23A) gene of the Giant Panda (Ailuropoda melanoleuca). The cDNA of RPL23A was cloned successfully from the Giant Panda using RT-PCR technology. We constructed a recombinant expression vector containing RPL23A cDNA and over-expressed it in Escherichia coli using pET28a plasmids. The expression product obtained was purified by using Ni chelating affinity chromatography. Recombinant protein of RPL23A obtained from the experiment acted on Hep-2 cells and human HepG-2 cells, then the growth inhibitory effect of these cells was observed by MTT (3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) assay. The result indicated that the length of the fragment cloned is 506 bp, and it contains an open-reading frame (ORF) of 471 bp encoding 156 amino acids. Primary structure analysis revealed that the molecular weight of the putative RPL23A protein is 17.719 kDa with a theoretical pI 11.16. The molecular weight of the recombinant protein RPL23A is 21.265 kDa with a theoretical pI 10.57. The RPL23A gene can be really expressed in E. coli and the RPL23A protein, fusioned with the N-terminally His-tagged protein, gave rise to the accumulation of an expected 22 KDa polypeptide. The data showed that the recombinant protein RPL23A had a time- and dose-dependency on the cell growth inhibition rate. The data also indicated that the effect at low concentrations was better than at high concentrations on Hep-2 cells, and that the concentration of 0.185 µg/mL had the best rate of growth inhibition of 36.31%. All results of the experiment revealed that the recombinant protein RPL23A exhibited anti-cancer function on the Hep-2 cells. The study provides a scientific basis and aids orientation for the research and development of cancer protein drugs as well as possible anti-cancer mechanisms. Further research is on going to determine the bioactive principle(s) of recombinant protein RPL23A responsible for its anticancer activity.