Hybrid nanoparticle composites applied to photodynamic therapy: strategies and applications.

Photodynamic therapy (PDT), as a robust strategy, has long been applied to cancer treatment owing to the meaningful breakthroughs and unique advantages, including ignorable invasiveness and spatio-temporal selectivity. Numerous PDT agents, especially hybrid nanoparticle composite (hybrid)-based sensitizers consisting of an organic polymer and inorganic nanoparticles (NPs), feature the synergetic pros of the components, which have unlocked the additional potentials of PDT. Although reviews relating to the applications of hybrids to PDT have been previously reported, most of them only focus on the designs of smart hybrids integrating multimodal imaging-guided multiple treatment modalities. Traditional PDT treatment has several limitations, such as inadequate PDT agents accumulating in cancer tissues, inferior PDT effect due to the devastating cancer hypoxia environment, relevant systemic toxicity in non-intelligent stimulation response treatment systems, and serious dependence of PDT on external light sources. Many strategies have been developed for overcoming these limitations, including improvement of cancer-homing ability by introducing active targeting groups, remodeling of the cancer hypoxia environment through oxygen regulators, intratumor release of ROS through activatable molecules, and replacement of laser light by X-rays or self-luminescence. This review aims to summarize the most recent advances in designing hybrids for improving the therapeutic efficacy of PDT.

An Oxygen Self-Evolving, Multistage Delivery System for Deeply Located Hypoxic Tumor Treatment.

The hypoxia-induced resistance to radiotherapy (RT) is a great threat to cancer patients. Therefore, overcoming the hypoxia tumor microenvironment is a vital issue. Herein, spindle-shaped CuS@CeO2 core-shell nanoparticles combining self-supplied oxygen, photothermal ability, and RT sensitive to cancer therapy are introduced. The spindle shape of CuS@CeO2 core-shell nanoparticles can potentiate their tumor penetration and subsequent internalization by cancer cells. The presence of CeO2 , functioning as a nanoenzyme, catalyzes the endogenous H2 O2 in tumor tissue into O2 , which remodels the hypoxic microenvironment into one susceptible to RT. CuS nanoparticles encapsulated in CeO2 undergo a steady release and deep tumor penetration, allowing the regression of lesions less affected by RT. Furthermore, in vitro and in vivo studies reveal that the design not only mitigates the dosage of RT, but more importantly allows the entire tumor to be treated without relapses.

Porous gold layer coated silver nanoplates with efficient antimicrobial activity.

Although silver nanoparticles are considered as promising antibacterial agents because of their antibacterial activity, the acute cytotoxicity of Ag+ released from Ag nanoparticles restricts their potential practical applications. Herein, porous Ag@Au nanoplates, which could balance the Ag+ release and the toxicity of Ag naoparticles, were fabricated by stepwise seed-mediated growth and oxidation. Laser irradiation further boosted their antimicrobial activity, and significantly accelerated the curing rate of wound. Comparing with Ag nanoplates, the irradiated porous Ag@Au nanoplates showed the similar antibiotic ability against S. aureus strains and lower cytotoxicity in vitro. When the porous Ag@Au nanoplates were applied to treat S. aureus-infected wound, they had the best curing effect. Thus, these porous Ag@Au nanoplates could act as promising antibacterial agents for wound healing applications.

Substitution of lysine at 627 position in PB2 protein does not change virulence of the 2009 pandemic H1N1 virus in mice.

A lysine at the 627 position (627K) of PB2 protein of influenza virus has been recognized as a determinant for host adaptation and a virulent element for some influenza viruses. While seasonal influenza viruses exclusively contained 627K, the pandemic (H1N1) 2009 possessed a glutamic acid (627E), even after circulation in humans for more than 6months. To explore the potential role of E627K substitution in PB2 in the pandemic (H1N1) 2009 virus, we compared pathogenicity and growth properties between a recombinant virus containing 627K PB2 gene and the parental A/California/4/2009 strain containing 627E. Our results showed that substitution of 627K in PB2 gene does not confer higher virulence and growth rate for the pandemic (H1N1) 2009 virus in mice and cell culture respectively, suggesting 627K is not required for human adaptation of the pandemic (H1N1) 2009 virus.