Enhancing volatile fatty acids production from waste activated sludge: The role of pretreatment by N,N-bis(carboxymethyl)-l-glutamate (GLDA).

N,N-bis(carboxymethyl)-l-glutamate (GLDA) is an eco-friendly chelating agent that effectively extracts multivalent metal ions from waste activated sludge (WAS) flocs, which could potentially alter their structure. However, the effect of GLDA on the production of volatile fatty acids (VFAs) from WAS is not well known. Here, we demonstrate that pretreatment with GLDA at a concentration of 200 mmol per kg VSS results in a significant increase of 142% in extractable extracellular polymeric substances and enhances the total VFAs yield by 64% compared to untreated samples. We reveal GLDA's capability to mobilize organic-binding multivalent metal ions within sludge flocs. Specifically, post-pretreatment analyses showed the release of 69.1 mg L-1 of Ca and 109.8 mg L-1 of Fe ions from the flocs, leading to a more relaxed floc structure and a reduced apparent activation energy (10.6 versus 20 kJ mol-1) for WAS solubilization. Molecular dynamic simulations further demonstrate GLDA's preferential binding to Fe3+ and Ca2+ over Mg2+. Our study suggests that GLDA pretreatment causes minimal disruption to reactor stability, thereby indicating the stability of microbial community composition. GLDA has emerged as a viable pretreatment agent for enhancing volatile fatty acids production from waste activated sludge.

Enhanced biological wastewater treatment supplemented with anaerobic fermentation liquid of primary sludge.

The wastewater treatment performance in an inverted A2/O reactor supplemented with fermentation liquid of primary sludge was explored comparing to commercial carbon sources sodium acetate and glucose. Similar COD removal rate was observed with the effluent COD stably reaching the discharge standard for those 3 carbon sources. However, the fermentation liquid distributed more carbon source in the anaerobic zone. Fermentation liquid and sodium acetate tests achieved better nitrogen removal rate than glucose test. The fermentation liquid test showed the best biological phosphorus removal performance with the effluent phosphorus barely reaching the discharge standard. The microbial community characterization revealed that the fermentation liquid test was dominated by phylum Proteobacter in all the anoxic, anaerobic and aerobic zones. Denitrifying phosphorus accumulating organisms (PAOs) (i.e., genera Dechloromonas and unclassified_f__Rhodocyclaceae) were selectively enriched with high abundances (over 20%), which resulted in improved phosphorus removal efficiency. Moreover, the predicted abundances of enzymes involved in nitrogen and phosphorus removal were also enhanced by the fermentation liquid.