RESUMO
We developed polymorphic microsatellite markers in Allium mongolicum Regel, a desert plant widely distributed in western China. To better conserve this species, we need to investigate its genetic diversity and population genetic structure, as well as its evolutionary history. Using the combined biotin capture technique, we isolated 13 novel polymorphic microsatellite loci and evaluated their characteristics using 60 individuals from two populations of A. mongolicum. Two to fourteen alleles per locus were identified for these microsatellites. The observed and expected heterozygosities ranged from 0.132 to 0.875 and 0.447 to 0.986, respectively. These microsatellite markers can be used to assess the genetic variation and genetic structure of A. mongolicum.
Assuntos
Allium/genética , DNA de Plantas/genética , Repetições de Microssatélites , DNA de Plantas/análise , Evolução Molecular , Genes de Plantas , Marcadores Genéticos , Variação Genética , Folhas de Planta/genética , Polimorfismo GenéticoRESUMO
This article documents the addition of 142 microsatellite marker loci to the Molecular Ecology Resources database. Loci were developed for the following species: Agriophyllum squarrosum, Amazilia cyanocephala, Batillaria attramentaria, Fungal strain CTeY1 (Ascomycota), Gadopsis marmoratus, Juniperus phoenicea subsp. turbinata, Liriomyza sativae, Lupinus polyphyllus, Metschnikowia reukaufii, Puccinia striiformis and Xylocopa grisescens. These loci were cross-tested on the following species: Amazilia beryllina, Amazilia candida, Amazilia rutila, Amazilia tzacatl, Amazilia violiceps, Amazilia yucatanensis, Campylopterus curvipennis, Cynanthus sordidus, Hylocharis leucotis, Juniperus brevifolia, Juniperus cedrus, Juniperus osteosperma, Juniperus oxycedrus, Juniperus thurifera, Liriomyza bryoniae, Liriomyza chinensis, Liriomyza huidobrensis and Liriomyza trifolii.
Assuntos
Biologia Computacional/métodos , Genômica/métodos , Repetições de Microssatélites , Animais , Abelhas/genética , Aves/genética , Peixes/genética , Fungos/genética , Plantas/genéticaRESUMO
In the title coordination polymer, [Ag(C(7)H(6)NO(2))(C(6)H(12)N(4))(H(2)O)](n), the Ag(I) ion is five-coordinated by two carboxyl-ate O atoms from one 4-amino-benzoate anion (L), two N atoms from two different hexa-methyl-enetetramine (hmt) ligands, and one water O atom in a distorted square-pyramidal geometry. The metal atom lies on a mirror plane and the L anion, hmt ligand and water mol-ecule all lie across crystallographic mirror planes. Each hmt ligand bridges two neighboring Ag(I) ions, resulting in the formation of a chain structure along the b axis. The chains are linked into a three-dimensional framework by N-Hâ¯O and O-Hâ¯O hydrogen bonds.
